Full Text Journal Articles by
Author Johannes H Hegemann

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Feed-borne Bacillus cereus exacerbates respiratory distress in chickens infected with Chlamydia psittaci by inducing haemorrhagic pneumonia.

Zonghui Zuo, Qiang Li, Yongxia Guo, Xiaohui Li, Shujian Huang, Johannes H Hegemann, Cheng He,

Chlamydia psittaci is an important zoonotic pathogen and its oral route of infection plays an important role in the transmission and persistence. Bacillus cereus (B. cereus) strain, a common contaminant of animal feed and feedstuffs, can cause severe diarrhoea and malnutrition in poultry. In our previous study, a B. cereus ... Read more >>

Avian Pathol. (Avian pathology : journal of the W.V.P.A)
[2020, 49(3):251-260]

Cited: 0 times

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Chlamydia-induced curvature of the host-cell plasma membrane is required for infection.

Sebastian Hänsch, Dominik Spona, Gido Murra, Karl Köhrer, Agathe Subtil, Ana Rita Furtado, Stephan F Lichtenthaler, Bastian Dislich, Katja Mölleken, Johannes H Hegemann,

During invasion of host cells, Chlamydia pneumoniae secretes the effector protein CPn0678, which facilitates internalization of the pathogen by remodeling the target cell's plasma membrane and recruiting sorting nexin 9 (SNX9), a central multifunctional endocytic scaffold protein. We show here that the strongly amphipathic N-terminal helix of CPn0678 mediates binding ... Read more >>

Proc. Natl. Acad. Sci. U.S.A. (Proceedings of the National Academy of Sciences of the United States of America)
[2020, 117(5):2634-2644]

Cited: 0 times

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A Chlamydia pneumoniae adhesin induces phosphatidylserine exposure on host cells.

Jan N Galle, Tim Fechtner, Thorsten Eierhoff, Winfried Römer, Johannes H Hegemann,

In mammalian cells, the internal and external leaflets of the plasma membrane (PM) possess different phospholipids. Phosphatidylserine (PS) is normally confined to the inner (cytoplasmic) membrane leaflet. Here we report that the adhesin CPn0473 of the human pathogenic bacterium Chlamydia pneumoniae (Cpn) binds to the PM of human cells and ... Read more >>

Nat Commun (Nature communications)
[2019, 10(1):4644]

Cited: 0 times

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Exofacial phospholipids at the plasma membrane: ill-defined targets for early infection processes.

Jan N Galle, Johannes H Hegemann,

The eukaryotic plasma membrane (PM) consists largely of phospholipids and proteins, and separates the intracellular compartments from the extracellular space. It also serves as a signaling platform for cell-to-cell communication and an interaction platform for the molecular crosstalk between pathogens and their target cells. Much research has been done to ... Read more >>

Biol. Chem. (Biological chemistry)
[2019, 400(10):1323-1334]

Cited: 0 times

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CPn0572, the C. pneumoniae ortholog of TarP, reorganizes the actin cytoskeleton via a newly identified F-actin binding domain and recruitment of vinculin.

Corinna Braun, Abel R Alcázar-Román, Alexandra Laska, Katja Mölleken, Ursula Fleig, Johannes H Hegemann,

Chlamydia pneumoniae is one of the two major species of the Chlamydiaceae family that have a profound effect on human health. C. pneumoniae is linked to a number of severe acute and chronic diseases of the upper and lower respiratory tract including pneumonia, asthma, bronchitis and infection by the pathogen ... Read more >>

PLoS ONE (PloS one)
[2019, 14(1):e0210403]

Cited: 1 time

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Vaginal Gel Component Hydroxyethyl Cellulose Significantly Enhances the Infectivity of Chlamydia trachomatis Serovars D and E.

Tímea Raffai, Katalin Burián, László Janovák, Anita Bogdanov, Johannes H Hegemann, Valéria Endrész, Dezső P Virok,

The transmission of the urogenital serovars of Chlamydia trachomatis can be significantly influenced by vaginal gels. Hydroxyethyl cellulose is a commonly used gelling agent that can be found in vaginal gels. Hydroxyethyl cellulose showed a concentration-dependent growth-enhancing effect on C. trachomatis serovars D and E, with a 26.1-fold maximal increase ... Read more >>

Antimicrob. Agents Chemother. (Antimicrobial agents and chemotherapy)
[2019, 63(1):]

Cited: 2 times

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The Chlamydia pneumoniae Tarp Ortholog CPn0572 Stabilizes Host F-Actin by Displacement of Cofilin.

Rafat Zrieq, Corinna Braun, Johannes H Hegemann,

Pathogenic Chlamydia species force entry into human cells via specific adhesin-receptor interactions and subsequently secrete effector proteins into the host cytoplasm, which in turn modulate host-cell processes to promote infection. One such effector, the C. trachomatis Tarp factor, nucleates actin polymerization in vitro. Here we show that its C. pneumoniae ... Read more >>

Front Cell Infect Microbiol (Frontiers in cellular and infection microbiology)
[2017, 7:511]

Cited: 2 times

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Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation.

Thomas Eder, Stefanie Kobus, Sonja Stallmann, Stefanie Stepanow, Karl Köhrer, Johannes H Hegemann, Thomas Rattei,

Chlamydia trachomatis (Ctr) is a bacterial pathogen that causes ocular, urogenital and lymph system infections in humans. It is highly abundant and among its serovars, E, F and D are most prevalent in sexually transmitted disease. However, the number of publicly available genome sequences of the serovars E and F, ... Read more >>

Pathog Dis (Pathogens and disease)
[2017, 75(9):]

Cited: 3 times

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Comparison of the nine polymorphic membrane proteins of Chlamydia trachomatis for their ability to induce protective immune responses in mice against a C. muridarum challenge.

Sukumar Pal, Alison Favaroni, Delia F Tifrea, Philipp T Hanisch, Sören E T Luczak, Johannes H Hegemann, Luis M de la Maza,

To test vaccines, formulated with novel antigens, to protect mice against Chlamydia infections.To determine the ability of polymorphic membrane proteins (Pmps) to induce cross-species protective immune responses, recombinant fragments from all nine C. trachomatis serovar E Pmps were used to vaccinate BALB/c mice utilizing CpG-1826 and Montanide ISA 720 as ... Read more >>

Vaccine (Vaccine)
[2017, 35(19):2543-2549]

Cited: 2 times

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Vaccination with the polymorphic membrane protein A reduces Chlamydia muridarum induced genital tract pathology.

Tina Müller, Elisabeth Becker, Sonja Stallmann, Anna Waldhuber, Franziska Römmler-Dreher, Simone Albrecht, Fabian Mohr, Johannes H Hegemann, Thomas Miethke,

Chlamydia trachomatis serovars D-K are one of the most frequent causes of sexually transmitted infections of the female genital tract, with possible complications such as hydrosalpinx, pelvic inflammatory disease, extra-uterine gravidity or infertility. We used the murine genital tract infection model with C. muridarum for vaccination studies and found that ... Read more >>

Vaccine (Vaccine)
[2017, 35(21):2801-2810]

Cited: 3 times

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A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C.

Aleksandra Inic-Kanada, Marijana Stojanovic, Emilija Marinkovic, Elisabeth Becker, Elisabeth Stein, Ivana Lukic, Radmila Djokic, Nadine Schuerer, Johannes H Hegemann, Talin Barisani-Asenbauer,

Recent advances in the development of chlamydia vaccines, using live-attenuated or ultraviolet light-inactivated chlamydia, are paving the way for new possibilities to oppose the societal challenges posed by chlamydia-related diseases, such as blinding trachoma. An effective subunit vaccine would mitigate the risks associated with the use of a whole-cell vaccine. ... Read more >>

PLoS ONE (PloS one)
[2016, 11(9):e0157875]

Cited: 4 times

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The Chlamydia trachomatis Ctad1 invasin exploits the human integrin β1 receptor for host cell entry.

Sonja Stallmann, Johannes H Hegemann,

Infection of human cells by the obligate intracellular bacterium Chlamydia trachomatis requires adhesion and internalization of the infectious elementary body (EB). This highly complex process is poorly understood. Here, we characterize Ctad1 (CT017) as a new adhesin and invasin from C. trachomatis serovar E. Recombinant Ctad1 (rCtad1) binds to human ... Read more >>

Cell. Microbiol. (Cellular microbiology)
[2016, 18(5):761-775]

Cited: 17 times

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Genome-wide Screen of Pseudomonas aeruginosa in Saccharomyces cerevisiae Identifies New Virulence Factors.

Rafat Zrieq, Thibault G Sana, Sandra Vergin, Steve Garvis, Irina Volfson, Sophie Bleves, Romé Voulhoux, Johannes H Hegemann,

Pseudomonas aeruginosa is a human opportunistic pathogen that causes mortality in cystic fibrosis and immunocompromised patients. While many virulence factors of this pathogen have already been identified, several remain to be discovered. In this respect we set an unprecedented genome-wide screen of a P. aeruginosa expression library based on a ... Read more >>

Front Cell Infect Microbiol (Frontiers in cellular and infection microbiology)
[2015, 5:81]

Cited: 3 times

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OmpA family proteins and Pmp-like autotransporter: new adhesins of Waddlia chondrophila.

Carole Kebbi-Beghdadi, Andreas Domröse, Elisabeth Becker, Ousmane H Cisse, Johannes H Hegemann, Gilbert Greub,

Waddlia chondrophila is a obligate intracellular bacterium belonging to the Chlamydiales order, a clade that also includes the well-known classical Chlamydia responsible for a number of severe human and animal diseases. Waddlia is an emerging pathogen associated with adverse pregnancy outcomes in humans and abortion in ruminants. Adhesion to the ... Read more >>

Pathog Dis (Pathogens and disease)
[2015, 73(6):ftv035]

Cited: 3 times

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Targeted gene deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Johannes H Hegemann, Sven Boris Heick, Jennifer Pöhlmann, Marcus M Langen, Ursula Fleig,

Gene deletion is an important element in the functional characterization of gene and protein function. Efficient tools for gene deletion have been developed in the model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, all of which rely on the replacement of the endogenous gene of interest with a selectable marker gene ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2014, 1163:45-73]

Cited: 2 times

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Characterization of the interaction between the chlamydial adhesin OmcB and the human host cell.

Tim Fechtner, Sonja Stallmann, Katja Moelleken, Klaus L Meyer, Johannes H Hegemann,

In a previous study, we reported that the OmcB protein from Chlamydia pneumoniae mediates adhesion of the infectious elementary body to human HEp-2 cells by interacting with heparin/heparan sulfate-like glycosaminoglycans (GAGs) via basic amino acids located in the first of a pair of XBBXBX heparin-binding motifs (K. Moelleken and J. ... Read more >>

J. Bacteriol. (Journal of bacteriology)
[2013, 195(23):5323-5333]

Cited: 19 times

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Missense variants in hMLH1 identified in patients from the German HNPCC consortium and functional studies.

Karin Hardt, Sven Boris Heick, Beate Betz, Timm Goecke, Haniyeh Yazdanparast, Robin Küppers, Kati Servan, Verena Steinke, Nils Rahner, Monika Morak, Elke Holinski-Feder, Christoph Engel, Gabriela Möslein, Hans-Konrad Schackert, Magnus von Knebel Doeberitz, Christian Pox, , , Johannes H Hegemann, Brigitte Royer-Pokora,

Missense mutations of the DNA mismatch repair gene MLH1 are found in a significant fraction of patients with Lynch syndrome (hereditary nonpolyposis colorectal cancer, HNPCC) and their pathogenicity often remains unclear. We report here all 88 MLH1 missense variants identified in families from the German HNPCC consortium with clinical details ... Read more >>

Fam. Cancer (Familial cancer)
[2011, 10(2):273-284]

Cited: 14 times

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Delete and repeat: a comprehensive toolkit for sequential gene knockout in the budding yeast Saccharomyces cerevisiae.

Johannes H Hegemann, Sven Boris Heick,

Gene inactivation is an essential step in the molecular dissection of gene function. In the yeast Saccharomyces cerevisiae, many tools for gene disruption are available. Gene disruption cassettes comprising completely heterologous marker genes flanked by short DNA segments homologous to the regions to the left and right of the gene ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2011, 765:189-206]

Cited: 34 times

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Transcriptional regulation of ASK/Dbf4 in cutaneous melanoma is dependent on E2F1.

Sandeep Nambiar, Alireza Mirmohammadsadegh, Mohamed Hassan, Johannes H Hegemann, Ulrich R Hengge,

BACKGROUND: Melanoma is a complex genetic disease, the management of which will require an in-depth understanding of the biology underlying its initiation and progression. Recently, we have reported the differential regulation of a novel gene, namely ASK/Dbf4, in melanoma and suggested upregulation of ASK/Dbf4 as a novel molecular determinant with ... Read more >>

Exp. Dermatol. (Experimental dermatology)
[2008, 17(12):986-991]

Cited: 2 times

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Identification and functional characterization of ASK/Dbf4, a novel cell survival gene in cutaneous melanoma with prognostic relevance.

Sandeep Nambiar, Alireza Mirmohammadsadegh, Mohamed Hassan, Rodrigo Mota, Alessandra Marini, Amine Alaoui, Andrea Tannapfel, Johannes H Hegemann, Ulrich R Hengge,

Malignant melanoma is one of the most aggressive and invasive metastatic tumors derived from melanocytes that have undergone malignant transformation by acquisition of genetic and epigenetic alterations. Oligonucleotide microarray-based screening of distinct stages in the tumor progression model of cutaneous melanoma identified ASK/Dbf4, as a novel determinant for melanoma development. ... Read more >>

Carcinogenesis (Carcinogenesis)
[2007, 28(12):2501-2510]

Cited: 17 times

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Mechanisms of Chlamydophila pneumoniae-mediated GM-CSF release in human bronchial epithelial cells.

Matthias Krüll, Petra Bockstaller, Frederik N Wuppermann, Andrea C Klucken, Jörg Mühling, Bernd Schmeck, Joachim Seybold, Clemens Walter, Matthias Maass, Simone Rosseau, Johannes H Hegemann, Norbert Suttorp, Stefan Hippenstiel,

Chlamydophila pneumoniae is an important respiratory pathogen. In this study we characterized C. pneumoniae strain TW183-mediated activation of human small airway epithelial cells (SAEC) and the bronchial epithelial cell line BEAS-2B and demonstrated time-dependent secretion of granulocyte macrophage colony-stimulating factor (GM-CSF) upon stimulation. TW183 activated p38 mitogen-activated protein kinase (MAPK) ... Read more >>

Am. J. Respir. Cell Mol. Biol. (American journal of respiratory cell and molecular biology)
[2006, 34(3):375-382]

Cited: 18 times

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Gene disruption in the budding yeast Saccharomyces cerevisiae.

Johannes H Hegemann, Ulrich Güldener, Gabriele J Köhler,

One essential step for the molecular dissection of gene function is gene inactivation. In the yeast Saccharomyces cerevisiae, elaborate tools for gene disruption are available. Gene disruption cassettes carrying completely heterologous marker genes flanked by short DNA segments homologous to the corresponding regions left and right of the gene to ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2006, 313:129-144]

Cited: 13 times

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HinT proteins and their putative interaction partners in Mollicutes and Chlamydiaceae.

Miriam Hopfe, Johannes H Hegemann, Birgit Henrich,

BACKGROUND: HinT proteins are found in prokaryotes and eukaryotes and belong to the superfamily of HIT proteins, which are characterized by an histidine-triad sequence motif. While the eukaryotic variants hydrolyze AMP derivates and modulate transcription, the function of prokaryotic HinT proteins is less clearly defined. In Mycoplasma hominis, HinT is ... Read more >>

BMC Microbiol. (BMC microbiology)
[2005, 5:27]

Cited: 1 time

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Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes.

Edith Bogengruber, Peter Briza, Edith Doppler, Herbert Wimmer, Lore Koller, Franco Fasiolo, Bruno Senger, Johannes H Hegemann, Michael Breitenbach,

An extensive homology search based on the sequence of the yeast protein Brx1p (biogenesis of ribosomes in Xenopus, YOL077c) revealed that it is a member of a superfamily of proteins sharing remarkable sequence similarities. Previous work on Brx1p showed that this protein is involved in the process of ribosome biogenesis ... Read more >>

FEMS Yeast Res. (FEMS yeast research)
[2003, 3(1):35-43]

Cited: 14 times

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Functional profiling of the Saccharomyces cerevisiae genome.

Guri Giaever, Angela M Chu, Li Ni, Carla Connelly, Linda Riles, Steeve Véronneau, Sally Dow, Ankuta Lucau-Danila, Keith Anderson, Bruno André, Adam P Arkin, Anna Astromoff, Mohamed El-Bakkoury, Rhonda Bangham, Rocio Benito, Sophie Brachat, Stefano Campanaro, Matt Curtiss, Karen Davis, Adam Deutschbauer, Karl-Dieter Entian, Patrick Flaherty, Francoise Foury, David J Garfinkel, Mark Gerstein, Deanna Gotte, Ulrich Güldener, Johannes H Hegemann, Svenja Hempel, Zelek Herman, Daniel F Jaramillo, Diane E Kelly, Steven L Kelly, Peter Kötter, Darlene LaBonte, David C Lamb, Ning Lan, Hong Liang, Hong Liao, Lucy Liu, Chuanyun Luo, Marc Lussier, Rong Mao, Patrice Menard, Siew Loon Ooi, Jose L Revuelta, Christopher J Roberts, Matthias Rose, Petra Ross-Macdonald, Bart Scherens, Greg Schimmack, Brenda Shafer, Daniel D Shoemaker, Sharon Sookhai-Mahadeo, Reginald K Storms, Jeffrey N Strathern, Giorgio Valle, Marleen Voet, Guido Volckaert, Ching-yun Wang, Teresa R Ward, Julie Wilhelmy, Elizabeth A Winzeler, Yonghong Yang, Grace Yen, Elaine Youngman, Kexin Yu, Howard Bussey, Jef D Boeke, Michael Snyder, Peter Philippsen, Ronald W Davis, Mark Johnston,

Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete collection of gene-deletion mutants (96% of annotated open reading frames, or ORFs) of the yeast Saccharomyces ... Read more >>

Nature (Nature)
[2002, 418(6896):387-391]

Cited: 2204 times

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