Full Text Journal Articles by
Author Ioanna Laios

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Tumor Banks: A Quality Control Scheme Proposal.

Ligia Craciun, Selim Alex Spinette, Marc Rassy, Roberto Salgado, Alexandre de Wind, Pieter Demetter, Laurine Verset, Maria Gomez-Galdon, Marie Chintinne, Nicolas Sirtaine, Nicolas de St Aubain, Ioanna Laios, Francoise Roy, Denis Larsimont,

Introduction: Tumor banks make a considerable contribution to translational research. Using emerging molecular tests on frozen material facilitates the development of new diagnostic and therapeutic strategies, especially in rare cases. However, standard quality control schemes are lacking in the current literature. Methods: In 2017, we have conducted a robust quality ... Read more >>

(Frontiers in medicine)
[2019, 6:225]

Cited: 0 times

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SETER/PR: a robust 18-gene predictor for sensitivity to endocrine therapy for metastatic breast cancer.

Bruno V Sinn, Chunxiao Fu, Rosanna Lau, Jennifer Litton, Tsung-Heng Tsai, Rashmi Murthy, Alda Tam, Eleni Andreopoulou, Yun Gong, Ravi Murthy, Rebekah Gould, Ya Zhang, Tari A King, Agnes Viale, Victor Andrade, Dilip Giri, Roberto Salgado, Ioanna Laios, Christos Sotiriou, Esmeralda C Marginean, Danielle N Kwiatkowski, Rachel M Layman, Daniel Booser, Christos Hatzis, V Vicente Valero, W Fraser Symmans,

There is a clinical need to predict sensitivity of metastatic hormone receptor-positive and HER2-negative (HR+/HER2-) breast cancer to endocrine therapy, and targeted RNA sequencing (RNAseq) offers diagnostic potential to measure both transcriptional activity and functional mutation. We developed the SETER/PR index to measure gene expression microarray probe sets that were ... Read more >>

(NPJ breast cancer)
[2019, 5:16]

Cited: 4 times

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Distinctive Desmoplastic 3D Morphology Associated With BRAFV600E in Papillary Thyroid Cancers.

Maxime Tarabichi, Aline Antoniou, Soazig Le Pennec, David Gacquer, Nicolas de Saint Aubain, Ligia Craciun, Thierry Cielen, Ioanna Laios, Denis Larsimont, Guy Andry, Jacques Emile Dumont, Carine Maenhaut, Vincent Detours,

Context:Although 60% of papillary thyroid carcinomas are BRAFV600E mutant (PTCV600E), the increased aggressiveness of these cancers is still debated. Objective:For PTCV600E we aimed to further characterize the extent of the stroma and its activation, the three-dimensional (3D) tumor-stroma interface, and the proliferation rates of tumor and stromal fibroblasts. Design:We analyzed ... Read more >>

J Clin Endocrinol Metab (The Journal of clinical endocrinology and metabolism)
[2018, 103(3):1102-1111]

Cited: 1 time

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The Genomic Grade Assay Compared With Ki67 to Determine Risk of Distant Breast Cancer Recurrence.

Michail Ignatiadis, Hatem A Azim, Christine Desmedt, Isabelle Veys, Denis Larsimont, Roberto Salgado, Maria B Lyng, Giuseppe Viale, Brian Leyland-Jones, Anita Giobbie-Hurder, Rosita Kammler, Patrizia Dell'Orto, Françoise Rothé, Ioanna Laïos, Henrik J Ditzel, Meredith M Regan, Martine Piccart, Stefan Michiels, Christos Sotiriou,

The Genomic Grade Index (GGI) was previously developed, evaluated on frozen tissue, and shown to be prognostic in early breast cancer. To test the GGI in formalin-fixed, paraffin-embedded breast cancer tumors, a quantitative reverse transcriptase polymerase chain reaction assay was developed and named the Genomic Grade (GG). The GG assay ... Read more >>

JAMA Oncol (JAMA oncology)
[2016, 2(2):217-224]

Cited: 11 times

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ERα dimerization: a key factor for the weak estrogenic activity of an ERα modulator unable to compete with estradiol in binding assays.

Guy Leclercq, Ioanna Laïos, Céline Elie-Caille, Denis Leiber, Guy Laurent, Eric Lesniewska, Zahra Tanfin, Yves Jacquot,

Estrothiazine (ESTZ) is a weak estrogen sharing structural similarities with coumestrol. ESTZ failed to compete with [3H]17β-estradiol ([3H]17β-E2) for binding to the estrogen receptor α (ERα), questioning its ability to interact with the receptor. However, detection by atomic force spectroscopy (AFS) of an ESTZ-induced ERα dimerization has eliminated any remaining ... Read more >>

J. Recept. Signal Transduct. Res. (Journal of receptor and signal transduction research)
[2017, 37(2):149-166]

Cited: 1 time

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Peptides targeting estrogen receptor alpha-potential applications for breast cancer treatment.

Guy Leclercq, Dominique Gallo, Janine Cossy, Ioanna Laïos, Denis Larsimont, Guy Laurent, Yves Jacquot,

Activation of the estrogen receptor alpha (ERα) is of prime importance for the development of hormone-dependent breast cancers. Hence, drugs able to impede the emergence of an active folding of ERα have been used for a long time as a first line therapeutic strategy. Aromatase inhibitors that block estradiol synthesis ... Read more >>

Curr. Pharm. Des. (Current pharmaceutical design)
[2011, 17(25):2632-2653]

Cited: 7 times

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A ferrocenyl derivative of hydroxytamoxifen elicits an estrogen receptor-independent mechanism of action in breast cancer cell lines.

Anne Vessières, Cyril Corbet, Jan Martin Heldt, Nicolas Lories, Nathalie Jouy, Ioanna Laïos, Guy Leclercq, Gérard Jaouen, Robert-Alain Toillon,

The aim of this work was to investigate the mechanism of action of ferrocifen (Fc-OH-TAM), the ferrocenyl analog of 4-hydroxy-tamoxifen (OH-TAM), which is the active metabolite of tamoxifen, the drug most widely prescribed for treatment of hormone-dependent breast cancers. Fc-OH-TAM showed an anti-proliferative effect on the six breast cancer cell ... Read more >>

J. Inorg. Biochem. (Journal of inorganic biochemistry)
[2010, 104(5):503-511]

Cited: 23 times

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Effect of fluorination on the pharmacological profile of 11beta isomers of fulvestrant in breast carcinoma cells.

Vangelis Agouridas, Emmanuel Magnier, Jean-Claude Blazejewski, Ioanna Laïos, Anny Cleeren, Denis Nonclercq, Guy Laurent, Guy Leclercq,

We describe the synthesis of an 11beta isomer 3 of the steroidal antiestrogen fulvestrant 2. Partial fluorination of the 11beta side chain in 3 leads to 4, which still shows strong antiproliferative activity on MCF-7 cells. However, unlike 2 and 3, compound 4 fails to down-regulate estrogen receptor alpha (ERalpha). ... Read more >>

J Med Chem (Journal of medicinal chemistry)
[2009, 52(3):883-887]

Cited: 3 times

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Capacity of type I and II ligands to confer to estrogen receptor alpha an appropriate conformation for the recruitment of coactivators containing a LxxLL motif-Relationship with the regulation of receptor level and ERE-dependent transcription in MCF-7 cells.

Sandrine Bourgoin-Voillard, Dominique Gallo, Ioanna Laïos, Anny Cleeren, Latifa El Bali, Yves Jacquot, Denis Nonclercq, Guy Laurent, Jean-Claude Tabet, Guy Leclercq,

Estrogen receptor alpha (ERalpha) belongs to the superfamily of nuclear receptors and as such acts as a ligand-modulated transcription factor. Ligands elicit in ERalpha conformational changes leading to the recruitment of coactivators required for the transactivation of target genes via cognate response elements. In many cells, activated ERalpha also undergoes ... Read more >>

Biochem. Pharmacol. (Biochemical pharmacology)
[2010, 79(5):746-757]

Cited: 13 times

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Trophic effect in MCF-7 cells of ERalpha17p, a peptide corresponding to a platform regulatory motif of the estrogen receptor alpha--underlying mechanisms.

Dominique Gallo, Iman Haddad, Hugues Duvillier, Françoise Jacquemotte, Ioanna Laïos, Guy Laurent, Yves Jacquot, Joëlle Vinh, Guy Leclercq,

As yet, estrogen receptor alpha (ERalpha) inhibitors used in clinical practice target a unique site, i.e. the hormone-binding pocket. With the aim of discovering other potential therapeutic targets in the receptor, we studied its AF-2a domain, a site that proves to be critical for ligand-independent ERalpha activity. Previous studies from ... Read more >>

J. Steroid Biochem. Mol. Biol. (The Journal of steroid biochemistry and molecular biology)
[2008, 109(1-2):138-149]

Cited: 11 times

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Effect of nuclear export inhibition on estrogen receptor regulation in breast cancer cells.

Denis Nonclercq, Fabrice Journé, Ioanna Laïos, Carole Chaboteaux, Robert-Alain Toillon, Guy Leclercq, Guy Laurent,

We used the Crm1 inhibitor leptomycin B (LMB) to examine a possible involvement of nuclear export in estrogen receptor alpha (ER) level and function in MCF-7 breast carcinoma cells. As revealed by immunofluorescence microscopy and western blotting with anti-ER antibodies, LMB produced an accumulation of ER in cell nuclei. LMB ... Read more >>

J. Mol. Endocrinol. (Journal of molecular endocrinology)
[2007, 39(2):105-118]

Cited: 15 times

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Calmodulin-independent, agonistic properties of a peptide containing the calmodulin binding site of estrogen receptor alpha.

Dominique Gallo, Françoise Jacquemotte, Anny Cleeren, Ioanna Laïos, Samira Hadiy, Martin G Rowlands, Olivier Caille, Denis Nonclercq, Guy Laurent, Yves Jacquot, Guy Leclercq,

Calmodulin (CaM) contributes to estrogen receptor alpha (ER)-mediated transcription. In order to study the underlying mechanisms, we synthesized a peptide including the CaM binding site: ERalpha17p (P(295)-T(311)). This peptide inhibited ER-CaM association, unlike two analogs in which two amino acids required for CaM binding were substituted. Exposure of MCF-7 cells ... Read more >>

Mol. Cell. Endocrinol. (Molecular and cellular endocrinology)
[2007, 268(1-2):37-49]

Cited: 13 times

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Synthesis, structure, and estrogenic activity of 4-amino-3-(2-methylbenzyl)coumarins on human breast carcinoma cells.

Yves Jacquot, Ioanna Laïos, Anny Cleeren, Denis Nonclercq, Laurent Bermont, Bernard Refouvelet, Kamal Boubekeur, Alain Xicluna, Guy Leclercq, Guy Laurent,

A number of coumarins exhibit interesting pharmacological activities and are therefore of therapeutic use. We report here the synthesis and the structural analysis of new N-substituted 4-amino-3-(2-methylbenzyl)coumarins (compounds 8a-8e) that present structural analogies with estrothiazine and 11- or 7-substituted 17beta-estradiol. These derivatives were tested with respect to estrogenic activity on ... Read more >>

Bioorg. Med. Chem. (Bioorganic & medicinal chemistry)
[2007, 15(6):2269-2282]

Cited: 11 times

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Fluorous tolerance of the estrogen receptor alpha as probed by 11-polyfluoroalkylestradiol derivatives.

Vangelis Agouridas, Jean-Claude Blazejewski, Anny Cleeren, Ioanna Laïos, Guy Leclercq, Emmanuel Magnier,

The concern of this work was to try to delineate factors, inherent to fluorination, susceptible to influence estradiol binding to the estrogen receptor alpha (ERalpha). For this purpose, fluorinated chains were linked at 11beta position of the steroid (i.e., C(6)F(13), CH(2)CH(2)C(4)F(9), CH(2)CH(2)C(8)F(17)). Relative binding affinity (RBA) for ERalpha of these ... Read more >>

Steroids (Steroids)
[2008, 73(3):320-327]

Cited: 3 times

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Effects of (R,S)/(S,R)-4,5-bis(2-chloro-4-hydroxyphenyl)-2-imidazolines and (R,S)/(S,R)-2,3-bis(2-chloro-4-hydroxyphenyl)piperazines on estrogen receptor alpha level and transcriptional activity in MCF-7 cells.

Ioanna Laïos, Anny Cleeren, Guy Leclercq, Denis Nonclercq, Guy Laurent, Miriam Schlenk, Anja Wellner, Ronald Gust,

4,5-Diaryl-2-imidazolines (Im(s)) and 2,3-diarylpiperazines (Pip(s)) belong to the type II class of estrogens. These compounds enhance ERalpha-mediated transcription of ERE-driven reporter genes in MCF-7 cells but do not compete with [(3)H]estradiol (E(2)) for receptor binding, because of distinct anchoring modes. The present study examined whether the estrogenic action of Im(s) ... Read more >>

Biochem. Pharmacol. (Biochemical pharmacology)
[2007, 74(7):1029-1038]

Cited: 6 times

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Estrogens decrease gamma-ray-induced senescence and maintain cell cycle progression in breast cancer cells independently of p53.

Robert-Alain Toillon, Nicolas Magné, Ioanna Laïos, Pierre Castadot, Eric Kinnaert, Paul Van Houtte, Christine Desmedt, Guy Leclercq, Marc Lacroix,

PURPOSE: Sequential administration of radiotherapy and endocrine therapy is considered to be a standard adjuvant treatment of breast cancer. Recent clinical reports suggest that radiotherapy could be more efficient in association with endocrine therapy. The aim of this study was to evaluate the estrogen effects on irradiated breast cancer cells ... Read more >>

Int. J. Radiat. Oncol. Biol. Phys. (International journal of radiation oncology, biology, physics)
[2007, 67(4):1187-1200]

Cited: 9 times

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Stimulatory effect of genistein and apigenin on the growth of breast cancer cells correlates with their ability to activate ER alpha.

Hye-Sook Seo, David G DeNardo, Yves Jacquot, Ioanna Laïos, Doris Salazar Vidal, Carmen Rojas Zambrana, Guy Leclercq, Powel H Brown,

Genistein and apigenin are phytoestrogens present in commercial preparations used for the treatment of postmenopausal symptoms. In this study, we assessed the influence of these compounds on mammary tumor growth. Both compounds stimulate the proliferation of MCF-7 and T47D cells [estrogen receptor alpha (ERalpha-positive)], but do not stimulate the proliferation ... Read more >>

Breast Cancer Res. Treat. (Breast cancer research and treatment)
[2006, 99(2):121-134]

Cited: 62 times

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Estrogen receptor alpha: impact of ligands on intracellular shuttling and turnover rate in breast cancer cells.

Guy Leclercq, Marc Lacroix, Ioanna Laïos, Guy Laurent,

Estrogen receptors (alpha and beta) are members of the steroid/thyroid nuclear receptors superfamily of ligand-dependent transcription factors. Impact of the alpha isoform of estrogen receptor (ER) on breast cancer etiology and progression is now well established. Current therapeutic strategy to treat ER-positive breast cancer relies on the blockade of ER ... Read more >>

Curr Cancer Drug Targets (Current cancer drug targets)
[2006, 6(1):39-64]

Cited: 46 times

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Loss of antagonistic activity of tamoxifen by replacement of one N-methyl of its side chain by fluorinated residues.

Vangelis Agouridas, Ioanna Laïos, Anny Cleeren, Elyane Kizilian, Emmanuel Magnier, Jean-Claude Blazejewski, Guy Leclercq,

Efforts to limit the metabolic alteration of the aminoalkyl side chain of tamoxifen by fluorination largely decrease its ER-mediated antagonistic properties in MCF-7 cells (i.e., ability to inhibit growth, to stabilize ER, and to modulate ERE and AP-1 transcriptional activity). This loss is associated with an enhancement of agonistic activity. ... Read more >>

Bioorg Med Chem (Bioorganic & medicinal chemistry)
[2006, 14(22):7531-7538]

Cited: 8 times

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Role of the proteasome in the regulation of estrogen receptor alpha turnover and function in MCF-7 breast carcinoma cells.

Ioanna Laïos, Fabrice Journé, Denis Nonclercq, Doris Salazar Vidal, Robert-Alain Toillon, Guy Laurent, Guy Leclercq,

Estrogen receptor alpha (ER) turnover in MCF-7 cells was assessed by pulse chase analysis and measurement of ER steady-state level. In untreated cells, degradation of (35)S-labeled ER was characterized by a slow phase followed by a more rapid decline. Without ligand, ER elimination was totally compensated by synthesis which maintained ... Read more >>

J Steroid Biochem Mol Biol (The Journal of steroid biochemistry and molecular biology)
[2005, 94(4):347-359]

Cited: 37 times

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Mechanisms governing the accumulation of estrogen receptor alpha in MCF-7 breast cancer cells treated with hydroxytamoxifen and related antiestrogens.

Ioanna Laïos, Fabrice Journe, Guy Laurent, Denis Nonclercq, Robert-Alain Toillon, Hye-Sook Seo, Guy Leclercq,

This study aimed at a better understanding of estrogen receptor alpha (ER) up regulation induced by partial estrogen antagonists. Effect of treatment with hydroxytamoxifen (OH-Tam) on ER level in MCF-7 cells was investigated by an approach combining ER measurement (enzyme immunoassay) and morphological demonstration (immunofluorescence). Furthermore, the influence of drug ... Read more >>

J. Steroid Biochem. Mol. Biol. (The Journal of steroid biochemistry and molecular biology)
[2003, 87(2-3):207-221]

Cited: 32 times

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Tamoxifen derivatives for delivery of the antitumoral (DACH)Pt group: selective synthesis by McMurry coupling, and biochemical behaviour.

Siden Top, El Bachir Kaloun, Anne Vessières, Guy Leclercq, Ioanna Laïos, Michèle Ourevitch, Christine Deuschel, Michael J McGlinchey, Gérard Jaouen,

The goal of our study was to potentiate the effects of the ((R,R)-trans-1,2-diaminocyclohexane)-platinum(II) fragment [(DACH)Pt], known for its cytotoxic properties, either with tamoxifen (Tam), the most widely used antiestrogen in the treatment of hormone-dependent breast cancers, or with its active metabolite hydroxytamoxifen (hydroxy-Tam). We coupled Tam or hydroxy-Tam derivatives bearing ... Read more >>

Chembiochem (Chembiochem : a European journal of chemical biology)
[2003, 4(8):754-761]

Cited: 13 times

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Pharmacological profile of 6,12-dihydro-3-methoxy-1-benzopyrano[3,4-b] [1,4]benzothiazin-6-one, a novel human estrogen receptor agonist.

Yves Jacquot, Anny Cleeren, Ioanna Laios, Ma Yan, Athem Boulahdour, Laurent Bermont, Bernard Refouvelet, Gérard Adessi, Guy Leclercq, Alain Xicluna,

Pharmacological studies were carried out to characterize further the endocrinological profile and the binding mode to the estrogen receptor (ER) of 6,12-dihydro-3-methoxy-1-benzopyrano[3,4-b][1,4]benzothiazin-6-one (1). Binding experiments were conducted with highly purified recombinant human estrogen receptors hERa and beta. Potent estrogenic activity of compound 1 was assessed by testing its ability to ... Read more >>

Biol. Pharm. Bull. (Biological & pharmaceutical bulletin)
[2002, 25(3):335-341]

Cited: 7 times

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Estrogenic effect of a series of bisphenol analogues on gene and protein expression in MCF-7 breast cancer cells.

Ana Rivas, Marc Lacroix, Fátima Olea-Serrano, Ioanna Laíos, Guy Leclercq, Nicolás Olea,

Bisphenols constitute a family of compounds, which includes many substances that have as a common chemical structure two phenolic rings joined together through a bridging carbon. In the present study, we aimed to determine whether several events triggered by 17 beta-estradiol (E(2)) in MCF-7 breast cancer cells were also observed ... Read more >>

J. Steroid Biochem. Mol. Biol. (The Journal of steroid biochemistry and molecular biology)
[2002, 82(1):45-53]

Cited: 42 times

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