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Author Idagene Cestari

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Impact of Vacuum-Assisted Venous Drainage on Forward Flow in Simulated Pediatric Cardiopulmonary Bypass Circuits Utilizing a Centrifugal Arterial Pump Head.

Daniel Peres Guimarães, Luiz Fernando Caneo, Gregory Matte, Luciana P Carletto, Valéria Camargo Policarpo, Ana Vitória C X Castro, Matheus H C Miranda, Priscila S Costa, Marcelo B Jatene, Idagene Cestari, Fabio B Jatene,

OBJECTIVE:To analyze the impact of vacuum-assisted venous drainage (VAVD) on arterial pump flow in a simulated pediatric cardiopulmonary bypass circuit utilizing a centrifugal pump (CP) with an external arterial filter. METHODS:The simulation circuit consisted of a Quadrox-I Pediatric oxygenator, a Rotaflow CP (Maquet Cardiopulmonary AG, Rastatt, Germany), and a custom ... Read more >>

Braz J Cardiovasc Surg (Brazilian journal of cardiovascular surgery)
[2020, 35(2):134-140]

Cited: 0 times

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Comparative study of strategies to prevent esophageal and periesophageal injury during atrial fibrillation ablation.

Barbara D de Oliveira, Helena Oyama, Carina A Hardy, Sissy L de Melo, Cristiano F Pisani, Muhieddine O Chokr, Conrado Balbo, Francisco C da Costa Darrieux, Denise T Hachul, Dalton M Chaves, Everson L de Almeida Artifon, Idágene A Cestari, Paulo Sakai, Mauricio I Scanavacca,

OBJECTIVE:To compare the prevalence of esophageal and periesophageal thermal injury in patients undergoing radiofrequency (RF) atrial fibrillation (AF) ablation using 8 mm tip catheters during three different esophageal protection strategies. METHODS:Forty-five consecutive patients with paroxysmal or persistent AF underwent first ablation procedure, besides esophagogastroduodenoscopy (EGD) combined with radial endosonography (EUS) performed ... Read more >>

J. Cardiovasc. Electrophysiol. (Journal of cardiovascular electrophysiology)
[2020, 31(4):924-933]

Cited: 0 times

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Functional Performance of Different Venous Limb Options in Simulated Neonatal/Pediatric Cardiopulmonary Bypass Circuits.

Luiz Fernando Caneo, Gregory S Matte, Daniel Peres Guimarães, Guilherme Viotto, Marcelo Mazzeto, Idagene Cestari, Rodolfo A Neirotti, Marcelo B Jatene, Shigang Wang, Akif Ündar, João Chang Junior, Fabio B Jatene,

OBJECTIVE:Hemodilution is a concern in cardiopulmonary bypass (CPB). Using a smaller dual tubing rather than a single larger inner diameter (ID) tubing in the venous limb to decrease prime volume has been a standard practice. The purpose of this study is to evaluate these tubing options. METHODS:Four different CPB circuits ... Read more >>

Braz J Cardiovasc Surg (Brazilian journal of cardiovascular surgery)
[2018, 33(3):224-232]

Cited: 2 times

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Integrated molecular, biochemical, and physiological assessment unravels key extraction method mediated influences on rat neonatal cardiomyocytes.

Leonardo Jensen, Elida Neri, Vinicius Bassaneze, Nathalia C De Almeida Oliveira, Rafael Dariolli, Lauro T Turaça, Débora Levy, Douglas Veronez, Mariana S A Ferraz, Adriano M Alencar, Sérgio P Bydlowski, Idágene A Cestari, José Eduardo Krieger,

Neonatal cardiomyocytes are instrumental for disease modeling, but the effects of different cell extraction methods on basic cell biological processes remain poorly understood. We assessed the influence of two popular methods to extract rat neonatal cardiomyocytes, Pre-plating (PP), and Percoll (PC) on cell structure, metabolism, and function. Cardiomyocytes obtained from ... Read more >>

J. Cell. Physiol. (Journal of cellular physiology)
[2018, 233(7):5420-5430]

Cited: 1 time

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In Vitro Evaluation of Pediatric Hollow-Fiber Membrane Oxygenators on Hemodynamic Performance and Gaseous Microemboli Handling: An International Multicenter/Multidisciplinary Approach.

Shigang Wang, Luiz F Caneo, Marcelo B Jatene, Fábio B Jatene, Idagene A Cestari, Allen R Kunselman, Akif Ündar,

The objective of this study was to compare the hemodynamic performances and gaseous microemboli (GME) handling ability of two pediatric oxygenators in a simulated pediatric cardiopulmonary bypass (CPB) model and the importance of adding an arterial filter in the circuit. The circuit consisted of a Braile Infant oxygenator or a ... Read more >>

Artif Organs (Artificial organs)
[2017, 41(9):865-874]

Cited: 2 times

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Computational tool for morphological analysis of cultured neonatal rat cardiomyocytes.

Maria Ruth C R Leite, Idágene A Cestari, Ismar N Cestari,

This study describes the development and evaluation of a semiautomatic myocyte edge-detector using digital image processing. The algorithm was developed in Matlab 6.0 using the SDC Morphology Toolbox. Its conceptual basis is the mathematical morphology theory together with the watershed and Euclidean distance transformations. The algorithm enables the user to ... Read more >>

Conf Proc IEEE Eng Med Biol Soc (Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference)
[2015, 2015:3517-3520]

Cited: 0 times

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Behavior of lyophilized biological valves in a chronic animal model.

Marina J S Maizato, Fabio P Taniguchi, Rafael F Ambar, Ronaldo N M Pitombo, Adolfo A Leirner, Idágene A Cestari, Noedir A G Stolf,

Glutaraldehyde is used in order to improve the mechanical and immunogenic properties of biological tissues, such as bovine pericardium membranes, used to manufacture heart valve bioprostheses. Lyophilization, also known as freeze-drying, preserves biological material without damage by freezing the water content and removing ice by sublimation. Through this process, dehydrated ... Read more >>

Artif Organs (Artificial organs)
[2013, 37(11):958-964]

Cited: 2 times

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Cavo-pulmonary anastomosis associated with left ventricular in comparison with biventricular circulatory support in acute heart failure.

Luis Alberto Saraiva Santos, Anderson Benício, Ewaldo de Mattos Júnior, Luiz Alberto Benvenutti, Idágene Aparecida Cestari, Noedir Antonio Groppo Stolf, Luiz Felipe Pinho Moreira,

OBJECTIVE:Right ventricular (RV) failure during left ventricular assist device (LVAD) support can result in severe hemodynamic compromise with high mortality. This study investigated the acute effects of cavo-pulmonary anastomosis on LVAD performance and RV myocardial compromise in comparison with biventricular circulatory support, in a model of biventricular failure. METHODS:LVAD support ... Read more >>

Rev Bras Cir Cardiovasc (Revista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular)
[2012, 27(4):552-561]

Cited: 5 times

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In vivo study of lyophilized bioprostheses: 3 month follow-up in young sheep.

Fábio Papa Taniguchi, Marina Junko Shiotsu Maizato, Rafael Fávero Ambar, Ronaldo Nogueira de Moraes Pitombo, Adolfo Alberto Leiner, Luiz Felipe Pinho Moreira, Idágene Aparecida Cestari, Noedir Antônio Groppo Stolf,

OBJECTIVE:Glutaraldehyde is currently used in bovine pericardium bioprosthesis to improve mechanical and immunogenic properties. Lyophilization is a process that may decrease aldehyde residues in the glutaraldehyde treated pericardium decreasing cytotoxicity and enhancing resistance to calcification. The aim of this study is to evaluate bioprosthetic heart valves calcification in adolescent sheep ... Read more >>

Rev Bras Cir Cardiovasc (Revista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular)
[2012, 27(4):592-599]

Cited: 1 time

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In vitro and in vivo evaluation of lyophilized bioprosthetic valve.

Marina J S Maizato, Sérgio A Hayashida, Fabio P Taniguchi, Olga Z Higa, Ronaldo N M Pitombo, Idágene A Cestari, Adolfo A Leirner, Noedir A G Stolf,

Freeze-drying of biological tissues allows for dry storage and gamma ray sterilization, which may improve their use as a medical prosthesis. The objective of this study was to evaluate the rehydration characteristics and hydrodynamic performance of prosthetic valves before and after lyophilization. Two size 23 bovine pericardium aortic valve prostheses ... Read more >>

Artif Organs (Artificial organs)
[2011, 35(5):490-496]

Cited: 4 times

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Spectral characteristics of atrial electrograms in sinus rhythm correlates with sites of ganglionated plexuses in patients with paroxysmal atrial fibrillation.

Esteban Wisnivesky Rocca Rivarola, Mauricio Scanavacca, Mauro Ushizima, Idágene Cestari, Carina Hardy, Sissy Lara, Cristiano Pisani, Eduardo Sosa,

AIMS: To verify whether spectral components of atrial electrograms (AE) during sinus rhythm (SR) correlate with cardiac ganglionated plexus (GP) sites. METHODS AND RESULTS: Thirteen patients undergoing atrial fibrillation (AF) ablation were prospectively enrolled. Prior to radio frequency application, endocardial AE were recorded with a sequential point-by-point approach. Electrical stimuli ... Read more >>

Europace (Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology)
[2011, 13(8):1141-1147]

Cited: 7 times

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A new approach to heart valve tissue engineering: mimicking the heart ventricle with a ventricular assist device in a novel bioreactor.

Andreas Kaasi, Idágene A Cestari, Noedir A G Stolf, Adolfo A Leirner, Ole Hassager, Ismar N Cestari,

The 'biomimetic' approach to tissue engineering usually involves the use of a bioreactor mimicking physiological parameters whilst supplying nutrients to the developing tissue. Here we present a new heart valve bioreactor, having as its centrepiece a ventricular assist device (VAD), which exposes the cell-scaffold constructs to a wider array of ... Read more >>

J Tissue Eng Regen Med (Journal of tissue engineering and regenerative medicine)
[2011, 5(4):292-300]

Cited: 1 time

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Analysis of hemodynamic performance of the bovine pericardium valved conduit, implanted in the aortic position in ovines.

Josalmir José Melo do Amaral, Pablo Maria Alberto Pomerantzeff, Ivan Sergio Joviano Casagrande, Idágene Aparecida Cestari, Paulo Sampaio Gutierrez, Noedir Groppo Stolf,

OBJECTIVE:The necessity for replacement of the valve, ascending aorta and aortic with coronary reimplantation in patients where anti-coagulation is undesirable, is increasing. We evaluated the hemodynamic performance of an aortic valved conduit made with glutaraldehyde treated bovine pericardium (AVCP) in animals. METHODS:Therefore, AVCPs were implanted in eight young ovine and ... Read more >>

Rev Bras Cir Cardiovasc (Revista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular)
[2010, 25(4):543-551]

Cited: 2 times

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Hemodynamic performance and inflammatory response during the use of VAD-InCor as a bridge to transplant.

João Galantier, Luiz Felipe P Moreira, Anderson Benício, Adolfo A Leirner, Idágene Cestari, Edmar A Bocchi, Fernando Bacal, Noedir A G Stolf,

BACKGROUND:Cardiac transplantation faces the serious problem of lack of donors and it is estimated that 20 to 40% of the patients die while waiting for heart transplantation. For these patients, the use of mechanical circulatory assist devices is the only choice of survival while waiting for a donor. In Brazil, ... Read more >>

Arq. Bras. Cardiol. (Arquivos brasileiros de cardiologia)
[2008, 91(5):327-334]

Cited: 6 times

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Adenoviral expression of calmodulin antisense reduces hypertrophy in cultured cardiomyocytes.

Ligia H Arruda, Idágene A Cestari, Adolfo A Leirner, Ismar N Cestari,

Sustained myocardial hypertrophy is associated with an increased risk of sudden death and progression to heart failure. Multiple signal pathways are involved in cardiac hypertrophy and understanding their interaction may point to new therapeutic targets. In this work, we tested the hypothesis that adenovirus-mediated calmodulin (CaM) antisense expression will reduce ... Read more >>

Artif Organs (Artificial organs)
[2007, 31(4):274-277]

Cited: 2 times

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Left ventricular circulatory support as bridge to heart transplantation in Chagas' disease cardiomyopathy.

Luiz Felipe P Moreira, João Galantier, Anderson Benício, Adolfo A Leirner, Idágene A Cestari, Noedir A G Stolf,

This study was performed to assess the safety and feasibility of the implantation of ventricular assist devices (VADs) as a bridge to heart transplantation in patients with advanced biventricular failure due to Chagas' disease. Six patients were submitted to paracorporeal left VAD implantation, while right ventricular dysfunction was managed clinically. ... Read more >>

Artif Organs (Artificial organs)
[2007, 31(4):253-258]

Cited: 10 times

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Echocardiographic assessment of global ventricular function using the myocardial performance index in rats with hypertrophy.

Vera M C Salemi, Mara D Pires, Ismar N Cestari, Idágene A Cestari, Michael H Picard, Adolfo A Leirner, Charles Mady,

Myocardial hypertrophy is the hallmark of chronic pressure overload and the myocardial performance index (MPI) is an easily recordable measurement of Doppler time intervals. In this study, the utility of using MPI to assess the progression of hypertrophy in the aortic-banded rat model was evaluated. Male Wistar rats (70-90 g) ... Read more >>

Artif Organs (Artificial organs)
[2004, 28(4):332-337]

Cited: 12 times

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Diabetes-induced alterations in latissimus dorsi muscle properties impair effectiveness of dynamic cardiomyoplasty in rats.

Kátia De Angelis, Sueli Senna, Maria Cláudia Irigoyen, Idágene A Cestari,

Short-term diabetes was induced in male Wistar rats with streptozotocin injection. The effects of diabetes on latissimus dorsi (LD) muscle contractile and biochemical properties and acute cardiomyoplasty (CDM) were assessed and compared with data from 16 control rats. Isometric force, contractile properties, and fatigue were measured in electrically stimulated muscles ... Read more >>

Artif Organs (Artificial organs)
[2004, 28(4):326-331]

Cited: 1 time

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Noninvasive assessment of hemodynamic parameters in experimental stenosis of the ascending aorta.

Mara D Pires, Vera M C Salemi, Idágene A Cestari, Michael H Picard, Adolfo A Leirner, Charles Mady, Ismar N Cestari,

We sought to noninvasively evaluate left ventricular (LV) function after cardiac hypertrophy induced by experimental stenosis of the ascending aorta. Male Wistar rats (70-90 g) underwent ascending aorta constriction by the surgical placement of a titanium clip (n=5) or sham operation (n=6). High-resolution bidimensional, pulsed-wave Doppler (PWD) and pulsed-wave tissue ... Read more >>

Artif Organs (Artificial organs)
[2003, 27(8):695-700]

Cited: 6 times

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Decreased 2,3-diphosphoglycerate concentration in low cardiac output patients and its influence on the determination of in vivo p50.

Marilde A Piccioni, Idágene A Cestari, Célia M C Strunz, José O Auler,

We investigated whether 2,3-diphosphoglycerate (2,3-DPG) is altered in patients with low cardiac output and the influence of its concentration on the calculation of in vivo P(50). Biochemical and blood gas analysis were performed along with the measurement of cardiac output and body temperature in 13 patients submitted to cardiopulmonary bypass ... Read more >>

Artif Organs (Artificial organs)
[2003, 27(8):676-680]

Cited: 2 times

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