Full Text Journal Articles by
Author Holger Barth

Advertisement

Find full text journal articles






CRISPA: A Non-viral, Transient Cas9 Delivery System Based on Reengineered Anthrax Toxin.

Maximilian Hirschenberger, Nicole Stadler, Maximilian Fellermann, Konstantin M J Sparrer, Frank Kirchhoff, Holger Barth, Panagiotis Papatheodorou,

Translating the CRISPR/Cas9 genome editing technology into clinics is still hampered by rather unspecific, unsafe and/or inconvenient approaches for the delivery of its main components - the Cas9 endonuclease and a guide RNA - into cells. Here, we describe the development of a novel transient and non-viral Cas9 delivery strategy ... Read more >>

Front Pharmacol (Frontiers in pharmacology)
[2021, 12:770283]

Cited: 0 times

View full text PDF listing >>



Human Peptides α-Defensin-1 and -5 Inhibit Pertussis Toxin.

Carolin Kling, Arto T Pulliainen, Holger Barth, Katharina Ernst,

<i>Bordetella pertussis</i> causes the severe childhood disease whooping cough, by releasing several toxins, including pertussis toxin (PT) as a major virulence factor. PT is an AB<sub>5</sub>-type toxin, and consists of the enzymatic A-subunit PTS1 and five B-subunits, which facilitate binding to cells and transport of PTS1 into the cytosol. PTS1 ... Read more >>

Toxins (Basel) (Toxins)
[2021, 13(7):]

Cited: 0 times

View full text PDF listing >>



Advertisement

Characterization and Pharmacological Inhibition of the Pore-Forming Clostridioides difficile CDTb Toxin.

Katharina Ernst, Marc Landenberger, Julian Nieland, Katharina Nørgaard, Manfred Frick, Giorgio Fois, Roland Benz, Holger Barth,

The clinically highly relevant <i>Clostridioides (C.) difficile</i> releases several AB-type toxins that cause diseases such as diarrhea and pseudomembranous colitis. In addition to the main virulence factors Rho/Ras-glycosylating toxins TcdA and TcdB, hypervirulent strains produce the binary AB-type toxin CDT. CDT consists of two separate proteins. The binding/translocation B-component CDTb ... Read more >>

Toxins (Basel) (Toxins)
[2021, 13(6):]

Cited: 0 times

View full text PDF listing >>



Cytotoxic Effects of Recombinant StxA2-His in the Absence of Its Corresponding B-Subunit.

Laura Heinisch, Maike Krause, Astrid Roth, Holger Barth, Herbert Schmidt,

AB<sub>5</sub> protein toxins are produced by certain bacterial pathogens and are composed of an enzymatically active A-subunit and a B-subunit pentamer, the latter being responsible for cell receptor recognition, cellular uptake, and transport of the A-subunit into the cytosol of eukaryotic target cells. Two members of the AB<sub>5</sub> toxin family ... Read more >>

Toxins (Basel) (Toxins)
[2021, 13(5):]

Cited: 0 times

View full text PDF listing >>



The cytotoxic effect of Clostridioides difficile pore-forming toxin CDTb.

Marc Landenberger, Julian Nieland, Maurice Roeder, Katharina Nørgaard, Panagiotis Papatheodorou, Katharina Ernst, Holger Barth,

Clostridioides (C.) difficile is clinically highly relevant and produces several AB-type protein toxins, which are the causative agents for C. difficile-associated diarrhea and pseudomembranous colitis. Treatment with antibiotics can lead to C. difficile overgrowth in the gut of patients due to the disturbed microbiota. C. difficile releases large Rho/Ras-GTPase glucosylating ... Read more >>

Biochim Biophys Acta Biomembr (Biochimica et biophysica acta. Biomembranes)
[2021, 1863(6):183603]

Cited: 1 time

View full text PDF listing >>



Pharmacological targeting of host chaperones protects from pertussis toxin in vitro and in vivo.

Katharina Ernst, Ann-Katrin Mittler, Veronika Winkelmann, Carolin Kling, Nina Eberhardt, Anna Anastasia, Michael Sonnabend, Robin Lochbaum, Jan Wirsching, Moona Sakari, Arto T Pulliainen, Ciaran Skerry, Nicholas H Carbonetti, Manfred Frick, Holger Barth,

Whooping cough is caused by Bordetella pertussis that releases pertussis toxin (PT) which comprises enzyme A-subunit PTS1 and binding/transport B-subunit. After receptor-mediated endocytosis, PT reaches the endoplasmic reticulum from where unfolded PTS1 is transported to the cytosol. PTS1 ADP-ribosylates G-protein α-subunits resulting in increased cAMP signaling. Here, a role of ... Read more >>

Sci Rep (Scientific reports)
[2021, 11(1):5429]

Cited: 2 times

View full text PDF listing >>



The enzyme subunit SubA of Shiga toxin-producing E. coli strains demonstrates comparable intracellular transport and cytotoxic activity as the holotoxin SubAB in HeLa and HCT116 cells in vitro.

Katharina Sessler, Panagiotis Papatheodorou, Fanny Wondany, Maike Krause, Sabrina Noettger, Denise Bernhard, Jens Michaelis, Herbert Schmidt, Holger Barth,

The subtilase cytotoxin (SubAB) is secreted by certain Shiga toxin-producing Escherichia coli (STEC) strains and is composed of the enzymatically active subunit SubA and the pentameric binding/transport subunit SubB. We previously demonstrated that SubA (10 µg/ml), in the absence of SubB, binds and intoxicates the human cervix cancer-derived epithelial cell line ... Read more >>

Arch Toxicol (Archives of toxicology)
[2021, 95(3):975-983]

Cited: 1 time

View full text PDF listing >>



A Nanodiamond-Based Theranostic Agent for Light-Controlled Intracellular Heating and Nanoscale Temperature Sensing

Yingke Wu, Md Noor Alam, Priyadharshini Balasubramanian, Anna Ermakova, Stefan Fischer, Holger Barth, Manfred Wagner, Marco Raabe, Fedor Jelezko, Tanja Weil,

Temperature is an essential factor in all biological processes and most of the biological activities are related to temperature. However, information about the temperature in living cells are limited. In photothermal therapy, an emerging cancer treatment due to its high spatial control and non-invasive nature, the local effect of intracellular ... Read more >>

()
[, :]

Cited: 0 times

View full text PDF listing >>



Intoxication of mammalian cells with binary clostridial enterotoxins is inhibited by the combination of pharmacological chaperone inhibitors.

Katharina Ernst, Judith Sailer, Maria Braune, Holger Barth,

Binary enterotoxins Clostridioides difficile CDT toxin, Clostridium botulinum C2 toxin, and Clostridium perfringens iota toxin consist of two separate protein components. The B-components facilitate receptor-mediated uptake into mammalian cells and form pores into endosomal membranes through which the enzymatic active A-components translocate into the cytosol. Here, the A-components ADP-ribosylate G-actin ... Read more >>

Naunyn Schmiedebergs Arch Pharmacol (Naunyn-Schmiedeberg's archives of pharmacology)
[2021, 394(5):941-954]

Cited: 0 times

View full text PDF listing >>



Pharmacological targeting of host chaperones protects from pertussis toxin in vitro and in vivo

Katharina Ernst, Ann-Katrin Mittler, Veronika Winkelmann, Nina Eberhardt, Anna Anastasia, Michael Sonnabend, Robin Lochbaum, Jan Wirsching, Ciaran Skerry, Nicholas Carbonetti, Manfred Frick, Holger Barth,

Whooping cough is caused by Bordetella pertussis that releases pertussis toxin (PT) which comprises enzyme A-subunit PTS1 and binding/transport B-subunit. After receptor-mediated endocytosis, PT reaches the endoplasmic reticulum from where unfolded PTS1 is transported to the cytosol. PTS1 ADP-ribosylates G-protein α-subunits resulting in increased cAMP signaling. Here, the role of ... Read more >>

()
[, :]

Cited: 0 times

View full text PDF listing >>



Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.

Maximilian Fellermann, Christina Huchler, Lea Fechter, Tobias Kolb, Fanny Wondany, Daniel Mayer, Jens Michaelis, Steffen Stenger, Kevin Mellert, Peter Möller, Thomas F E Barth, Stephan Fischer, Holger Barth,

C3 protein toxins produced by <i>Clostridium (C.) botulinum</i> and <i>C. limosum</i> are mono-ADP-ribosyltransferases, which specifically modify the GTPases Rho A/B/C in the cytosol of monocytic cells, thereby inhibiting Rho-mediated signal transduction in monocytes, macrophages, and osteoclasts. C3 toxins are selectively taken up into the cytosol of monocytic cells by endocytosis ... Read more >>

Toxins (Basel) (Toxins)
[2020, 12(9):]

Cited: 0 times

View full text PDF listing >>



Human α-Defensin-5 Efficiently Neutralizes Clostridioides difficile Toxins TcdA, TcdB, and CDT.

Michael Korbmacher, Stephan Fischer, Marc Landenberger, Panagiotis Papatheodorou, Klaus Aktories, Holger Barth,

Infections with the pathogenic bacterium <i>Clostridioides (C.) difficile</i> are coming more into focus, in particular in hospitalized patients after antibiotic treatment. <i>C. difficile</i> produces the exotoxins TcdA and TcdB. Since some years, hypervirulent strains are described, which produce in addition the binary actin ADP-ribosylating toxin CDT. These strains are associated ... Read more >>

Front Pharmacol (Frontiers in pharmacology)
[2020, 11:1204]

Cited: 3 times

View full text PDF listing >>



Bacillus anthracis' PA63 Delivers the Tumor Metastasis Suppressor Protein NDPK-A/NME1 into Breast Cancer Cells.

Ina Felix, Santosh K Lomada, Holger Barth, Thomas Wieland,

Some highly metastatic types of breast cancer show decreased intracellular levels of the tumor suppressor protein NME1, also known as nm23-H1 or nucleoside diphosphate kinase A (NDPK-A), which decreases cancer cell motility and metastasis. Since its activity is directly correlated with the overall outcome in patients, increasing the cytosolic levels ... Read more >>

Int J Mol Sci (International journal of molecular sciences)
[2020, 21(9):]

Cited: 0 times

View full text PDF listing >>



Human peptide α-defensin-1 interferes with Clostridioides difficile toxins TcdA, TcdB, and CDT.

Stephan Fischer, Anna-Katharina Ückert, Marc Landenberger, Panagiotis Papatheodorou, Carola Hoffmann-Richter, Ann-Katrin Mittler, Ulrich Ziener, Marlen Hägele, Carsten Schwan, Martin Müller, Alexander Kleger, Roland Benz, Michel R Popoff, Klaus Aktories, Holger Barth,

The human pathogenic bacterium Clostridioides difficile produces two exotoxins TcdA and TcdB, which inactivate Rho GTPases thereby causing C. difficile-associated diseases (CDAD) including life-threatening pseudomembranous colitis. Hypervirulent strains produce additionally the binary actin ADP-ribosylating toxin CDT. These strains are hallmarked by more severe forms of CDAD and increased frequency and ... Read more >>

FASEB J (FASEB journal : official publication of the Federation of American Societies for Experimental Biology)
[2020, 34(5):6244-6261]

Cited: 7 times

View full text PDF listing >>



Super-resolution microscopy unveils transmembrane domain-mediated internalization of cross-reacting material 197 into diphtheria toxin-resistant mouse J774A.1 cells and primary rat fibroblasts in vitro.

Maximilian Fellermann, Fanny Wondany, Stefan Carle, Julia Nemeth, Tanmay Sadhanasatish, Manfred Frick, Holger Barth, Jens Michaelis,

Diphtheria toxin (DT) efficiently inhibits protein synthesis in human cells, resulting in severe disease diphtheria. The sensitivity towards DT varies between mammalian species. Mice and rats are resistant to DT. However, the reason underlying this insensitivity is controversially discussed and not well understood. Therefore, we investigated the steps of DT ... Read more >>

Arch Toxicol (Archives of toxicology)
[2020, 94(5):1753-1761]

Cited: 1 time

View full text PDF listing >>



Regulation of endo-lysosomal pathway and autophagic flux by broad-spectrum antipathogen inhibitor ABMA.

Yu Wu, Claire Boulogne, Stefan Carle, Maria Podinovskaia, Holger Barth, Anne Spang, Jean-Christophe Cintrat, Daniel Gillet, Julien Barbier,

The endo-lysosome system is involved in endocytosis, protein sorting, and degradation as well as autophagy. Numerous toxins and pathogens exploit this system to enter host cells and exert their deleterious effects. Modulation of host endo-lysosome pathway may restrict multiple toxins intoxication as well as pathogen infection. ABMA, selected from a ... Read more >>

FEBS J (The FEBS journal)
[2020, 287(15):3184-3199]

Cited: 1 time

View full text PDF listing >>



Variants of Escherichia coli Subtilase Cytotoxin Subunits Show Differences in Complex Formation In Vitro.

Maike Krause, Katharina Sessler, Anna Kaziales, Richard Grahl, Sabrina Noettger, Holger Barth, Herbert Schmidt,

The subtilase cytotoxin (SubAB) of Shiga toxin-producing Escherichia coli (STEC) is a member of the AB5 toxin family. In the current study, we analyzed the formation of active homo- and hetero-complexes of SubAB variants in vitro to characterize the mode of assembly of the subunits. Recombinant SubA1-His, SubB1-His, SubA2-2-His, and ... Read more >>

Toxins (Basel) (Toxins)
[2019, 11(12):]

Cited: 2 times

View full text PDF listing >>



The Antibiotic Bacitracin Protects Human Intestinal Epithelial Cells and Stem Cell-Derived Intestinal Organoids from Clostridium difficile Toxin TcdB.

Ziyu Zhu, Leonie Schnell, Bastian Müller, Martin Müller, Panagiotis Papatheodorou, Holger Barth,

Bacitracin is an established antibiotic for local application and inhibits the cell wall synthesis of Gram-positive bacteria. Recently, we discovered a completely different mode of action of bacitracin and reported that this drug protects human cells from intoxication by a variety of medically relevant bacterial protein toxins including CDT, the ... Read more >>

Stem Cells Int (Stem cells international)
[2019, 2019:4149762]

Cited: 4 times

View full text PDF listing >>



Supramolecular Toxin Complexes for Targeted Pharmacological Modulation of Polymorphonuclear Leukocyte Functions.

Astrid Johanna Heck, Theresa Ostertag, Leonie Schnell, Stephan Fischer, Bikram Keshari Agrawalla, Pia Winterwerber, Eva Wirsching, Michael Fauler, Manfred Frick, Seah Ling Kuan, Tanja Weil, Holger Barth,

The targeted pharmacological modulation of polymorphonuclear leukocytes (PMNs) is of major medical interest. These innate immune cells play a central role in the defense against pathogenic microorganisms. However, their excessive chemotactic recruitment into tissues after traumatic injury is detrimental due to local and systemic inflammation. Rho-GTPases, being the master regulators ... Read more >>

Adv Healthc Mater (Advanced healthcare materials)
[2019, 8(17):e1900665]

Cited: 1 time

View full text PDF listing >>



Cyclic Changes in the Amide Bands Within Escherichia coli Biofilms Monitored Using Real-Time Infrared Attenuated Total Reflection Spectroscopy (IR-ATR).

Pavla Stenclova, Simon Freisinger, Holger Barth, Alexander Kromka, Boris Mizaikoff,

Contrary to the planktonic state of bacteria, their biofilm form represents severe complications in areas such as human medicine or food industry due to the increasing resistance against harsh conditions and treatment. In the present study, infrared attenuated total reflection (IR-ATR) spectroscopy has been applied as an analytic tool studying ... Read more >>

Appl Spectrosc (Applied spectroscopy)
[2019, 73(4):424-432]

Cited: 1 time

View full text PDF listing >>



Revisiting an old antibiotic: bacitracin neutralizes binary bacterial toxins and protects cells from intoxication.

Leonie Schnell, Ina Felix, Bastian Müller, Mirko Sadi, Franziska von Bank, Panagiotis Papatheodorou, Michel R Popoff, Klaus Aktories, Eva Waltenberger, Roland Benz, Conrad Weichbrodt, Michael Fauler, Manfred Frick, Holger Barth,

The antibiotic bacitracin (Bac) inhibits cell wall synthesis of gram-positive bacteria. Here, we discovered a totally different activity of Bac: the neutralization of bacterial exotoxins. Bac prevented intoxication of mammalian cells with the binary enterotoxins Clostridium botulinum C2, C. perfringens ι, C. difficile transferase (CDT), and Bacillus anthracis lethal toxin. ... Read more >>

FASEB J (FASEB journal : official publication of the Federation of American Societies for Experimental Biology)
[2019, 33(4):5755-5771]

Cited: 3 times

View full text PDF listing >>



Prognostic impact of polypharmacy and drug interactions in patients with advanced cancer.

Alexander Hoemme, Holger Barth, Manuel Haschke, Stephan Krähenbühl, Florian Strasser, Claudia Lehner, Alexander von Kameke, Thomas Wälti, Beat Thürlimann, Martin Früh, Christoph Driessen, Markus Joerger,

The risk of potential drug-drug interactions (PDI) is poorly studied in oncology. We included 105 patients with advanced non-small-cell lung cancer (NSCLC), 100 patients with advanced breast cancer (BC) and 100 patients of the palliative care unit (PCU) receiving systemic palliative treatment between 2010 and 2015. All patients suffered from ... Read more >>

Cancer Chemother Pharmacol (Cancer chemotherapy and pharmacology)
[2019, 83(4):763-774]

Cited: 2 times

View full text PDF listing >>



Autonomous Ultrafast Self-Healing Hydrogels by pH-Responsive Functional Nanofiber Gelators as Cell Matrices.

Jasmina Gačanin, Jana Hedrich, Stefanie Sieste, Gunnar Glaßer, Ingo Lieberwirth, Corinna Schilling, Stephan Fischer, Holger Barth, Bernd Knöll, Christopher V Synatschke, Tanja Weil,

The synthesis of hybrid hydrogels by pH-controlled structural transition with exceptional rheological properties as cellular matrix is reported. "Depsi" peptide sequences are grafted onto a polypeptide backbone that undergo a pH-induced intramolecular O-N-acyl migration at physiological conditions affording peptide nanofibers (PNFs) as supramolecular gelators. The polypeptide-PNF hydrogels are mechanically remarkably ... Read more >>

Adv Mater (Advanced materials (Deerfield Beach, Fla.))
[2019, 31(2):e1805044]

Cited: 7 times

View full text PDF listing >>



Combined Pharmacological Inhibition of Cyclophilins, FK506-Binding Proteins, Hsp90, and Hsp70 Protects Cells From Clostridium botulinum C2 Toxin.

Katharina Ernst, Carolin Kling, Marc Landenberger, Holger Barth,

The <i>Clostridium botulinum</i> C2 toxin is an exotoxin causing severe enterotoxic symptoms. The C2 toxin consists of the binding/translocation component C2II, and the enzymatic active component C2I. After proteolytic activation, C2IIa forms heptamers that bind C2I. The C2IIa/C2I complex is taken up into mammalian target cells via receptor-mediated endocytosis. Acidification ... Read more >>

Front Pharmacol (Frontiers in pharmacology)
[2018, 9:1287]

Cited: 2 times

View full text PDF listing >>



Primary resistance of human patients to botulinum neurotoxins A and B.

Marco Pirazzini, Stefan Carle, Holger Barth, Ornella Rossetto, Cesare Montecucco,

Botulinum neurotoxin serotypes A and B are successfully used to treat a variety of human diseases characterized by hyperactive peripheral nerve terminals. However, a number of patients are primary resistant to these pharmaceuticals, without having antitoxin-neutralizing antibodies. A straightforward explanation of this phenomenon posits that mutations of the toxin sites ... Read more >>

Ann Clin Transl Neurol (Annals of clinical and translational neurology)
[2018, 5(8):971-975]

Cited: 2 times

View full text PDF listing >>



Advertisement


Disclaimer

1.127 s