Full Text Journal Articles by
Author Hans Oberleithner


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Quantifying salt sensitivity.

Hans Oberleithner,

Inner surfaces of blood vessels and outer surfaces of erythrocytes are coated with a negatively charged protective film of proteoglycans, which serves as an effective buffer system for the positively charged sodium ions. If this protective coating is poorly developed or impaired, it loses its buffering capacity. As a consequence, ... Read more >>

Biol Chem (Biological chemistry)
[2021, 402(12):1597-1602]

Cited: 0 times

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Intravascular adhesion and recruitment of neutrophils in response to CXCL1 depends on their TRPC6 channels.

Otto Lindemann, Jan Rossaint, Karolina Najder, Sandra Schimmelpfennig, Verena Hofschröer, Mike Wälte, Benedikt Fels, Hans Oberleithner, Alexander Zarbock, Albrecht Schwab,

Here we report a novel role for TRPC6, a member of the transient receptor potential (TRPC) channel family, in the CXCL1-dependent recruitment of murine neutrophil granulocytes. Representing a central element of the innate immune system, neutrophils are recruited from the blood stream to a site of inflammation. The recruitment process ... Read more >>

J Mol Med (Berl) (Journal of molecular medicine (Berlin, Germany))
[2020, 98(3):349-360]

Cited: 3 times

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A two-phase response of endothelial cells to hydrostatic pressure.

Valeria Prystopiuk, Benedikt Fels, Caroline Sophie Simon, Ivan Liashkovich, Dzmitry Pasrednik, Cornelius Kronlage, Roland Wedlich-Söldner, Hans Oberleithner, Johannes Fels,

The vascular endothelium is exposed to three types of mechanical forces: blood flow-mediated shear stress, vessel diameter-dependent wall tension and hydrostatic pressure. Despite considerable variations of blood pressure during normal and pathological physiology, little is known about the acute molecular and cellular effects of hydrostatic pressure on endothelial cells. Here, ... Read more >>

J Cell Sci (Journal of cell science)
[2018, 131(12):]

Cited: 11 times

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KCa3.1 channel inhibition leads to an ICAM-1 dependent increase of cell-cell adhesion between A549 lung cancer and HMEC-1 endothelial cells.

Etmar Bulk, Nadzeya Kramko, Ivan Liashkovich, Felix Glaser, Hermann Schillers, Hans-Joachim Schnittler, Hans Oberleithner, Albrecht Schwab,

Early metastasis leads to poor prognosis of lung cancer patients, whose 5-year survival rate is only 15%. We could recently show that the Ca<sup>2+</sup> sensitive K<sup>+</sup> channel K<sub>Ca</sub>3.1 promotes aggressive behavior of non-small cell lung cancer (NSCLC) cells and that it can serve as a prognostic marker in NSCLC. Since ... Read more >>

Oncotarget (Oncotarget)
[2017, 8(68):112268-112282]

Cited: 6 times

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Standardized Nanomechanical Atomic Force Microscopy Procedure (SNAP) for Measuring Soft and Biological Samples.

Hermann Schillers, Carmela Rianna, Jens Schäpe, Tomas Luque, Holger Doschke, Mike Wälte, Juan José Uriarte, Noelia Campillo, Georgios P A Michanetzis, Justyna Bobrowska, Andra Dumitru, Elena T Herruzo, Simone Bovio, Pierre Parot, Massimiliano Galluzzi, Alessandro Podestà, Luca Puricelli, Simon Scheuring, Yannis Missirlis, Ricardo Garcia, Michael Odorico, Jean-Marie Teulon, Frank Lafont, Malgorzata Lekka, Felix Rico, Annafrancesca Rigato, Jean-Luc Pellequer, Hans Oberleithner, Daniel Navajas, Manfred Radmacher,

We present a procedure that allows a reliable determination of the elastic (Young's) modulus of soft samples, including living cells, by atomic force microscopy (AFM). The standardized nanomechanical AFM procedure (SNAP) ensures the precise adjustment of the AFM optical lever system, a prerequisite for all kinds of force spectroscopy methods, ... Read more >>

Sci Rep (Scientific reports)
[2017, 7(1):5117]

Cited: 42 times

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Endothelial glycocalyx breakdown is mediated by angiopoietin-2.

Alexander Lukasz, Carina Hillgruber, Hans Oberleithner, Kristina Kusche-Vihrog, Hermann Pavenstädt, Alexandros Rovas, Bettina Hesse, Tobias Goerge, Philipp Kümpers,

<h4>Aims</h4>The endothelial glycocalyx (eGC), a carbohydrate-rich layer lining the luminal surface of the endothelium, provides a first vasoprotective barrier against vascular leakage and adhesion in sepsis and vessel inflammation. Angiopoietin-2 (Angpt-2), an antagonist of the endothelium-stabilizing receptor Tie2 secreted by endothelial cells, promotes vascular permeability through cellular contraction and junctional ... Read more >>

Cardiovasc Res (Cardiovascular research)
[2017, 113(6):671-680]

Cited: 40 times

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Endothelial basement membrane laminin 511 is essential for shear stress response.

Jacopo Di Russo, Anna-Liisa Luik, Lema Yousif, Sigmund Budny, Hans Oberleithner, Verena Hofschröer, Juergen Klingauf, Ed van Bavel, Erik Ntp Bakker, Per Hellstrand, Anirban Bhattachariya, Sebastian Albinsson, Frederic Pincet, Rupert Hallmann, Lydia M Sorokin,

EMBO J (The EMBO journal)
[2017, 36(10):1464]

Cited: 2 times

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Extracellular protonation modulates cell-cell interaction mechanics and tissue invasion in human melanoma cells.

Verena Hofschröer, Kevin Alexander Koch, Florian Timo Ludwig, Peter Friedl, Hans Oberleithner, Christian Stock, Albrecht Schwab,

Detachment of cells from the primary tumour precedes metastatic progression by facilitating cell release into the tissue. Solid tumours exhibit altered pH homeostasis with extracellular acidification. In human melanoma, the Na<sup>+</sup>/H<sup>+</sup> exchanger NHE1 is an important modifier of the tumour nanoenvironment. Here we tested the modulation of cell-cell-adhesion by extracellular ... Read more >>

Sci Rep (Scientific reports)
[2017, 7:42369]

Cited: 18 times

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Endothelial basement membrane laminin 511 is essential for shear stress response.

Jacopo Di Russo, Anna-Liisa Luik, Lema Yousif, Sigmund Budny, Hans Oberleithner, Verena Hofschröer, Juergen Klingauf, Ed van Bavel, Erik Ntp Bakker, Per Hellstrand, Anirban Bhattachariya, Sebastian Albinsson, Frederic Pincet, Rupert Hallmann, Lydia M Sorokin,

Shear detection and mechanotransduction by arterial endothelium requires junctional complexes containing PECAM-1 and VE-cadherin, as well as firm anchorage to the underlying basement membrane. While considerable information is available for junctional complexes in these processes, gained largely from in vitro studies, little is known about the contribution of the endothelial basement ... Read more >>

EMBO J (The EMBO journal)
[2017, 36(2):183-201]

Cited: 18 times

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Salt Sensitivity Determined From Capillary Blood.

Hans Oberleithner, Marianne Wilhelmi,

<h4>Background/aims</h4>A significant rise of blood pressure in response to a given salt load is a weak indication of high salt sensitivity, supposed to foster the development of arterial hypertension and related diseases in later life. In search of an alternative method we recently developed the salt blood test (SBT), a ... Read more >>

Kidney Blood Press Res (Kidney & blood pressure research)
[2016, 41(4):355-364]

Cited: 1 time

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Differential response to endothelial epithelial sodium channel inhibition ex vivo correlates with arterial stiffness in humans.

Malte Lenders, Verena Hofschröer, Boris Schmitz, Bernd Kasprzak, Astrid Rohlmann, Markus Missler, Hermann Pavenstädt, Hans Oberleithner, Stefan-Martin Brand, Kristina Kusche-Vihrog, Eva Brand,

<h4>Objectives</h4>Recently, the nanomechanical properties (i.e. stiffness) of endothelial cells have been identified as crucial for appropriate endothelial function. One major determinant of endothelial stiffness is the endothelial sodium channel (EnNaC). EnNaC-dependent stiffening leads to reduced nitric oxide release, which is a hallmark for endothelial dysfunction. In the current study, we ... Read more >>

J Hypertens (Journal of hypertension)
[2015, 33(12):2455-2462]

Cited: 7 times

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Feeling for Filaments: Quantification of the Cortical Actin Web in Live Vascular Endothelium.

Cornelius Kronlage, Marco Schäfer-Herte, Daniel Böning, Hans Oberleithner, Johannes Fels,

Contact-mode atomic force microscopy (AFM) has been shown to reveal cortical actin structures. Using live endothelial cells, we visualized cortical actin dynamics simultaneously by AFM and confocal fluorescence microscopy. We present a method that quantifies dynamic changes in the mechanical ultrastructure of the cortical actin web. We argue that the ... Read more >>

Biophys J (Biophysical journal)
[2015, 109(4):687-698]

Cited: 21 times

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Cystic fibrosis transmembrane conductance regulator is involved in polyphenol-induced swelling of the endothelial glycocalyx.

Wladimir Peters, Kristina Kusche-Vihrog, Hans Oberleithner, Hermann Schillers,

<h4>Unlabelled</h4>Previous studies show that polyphenol-rich compounds can induce a swelling of the endothelial glycocalyx (eGC). Our goal was to reveal the mechanism behind the eGC-swelling. As polyphenols are potent modulators of fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel, the hypothesis was tested whether polyphenol-induced increase in CFTR activity is responsible ... Read more >>

Nanomedicine (Nanomedicine : nanotechnology, biology, and medicine)
[2015, 11(6):1521-1530]

Cited: 9 times

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Sodium renders endothelial cells sticky for red blood cells.

Hans Oberleithner, Mike Wälte, Kristina Kusche-Vihrog,

Negative charges in the glycocalyx of red blood cells (RBC) and vascular endothelial cells (EC) facilitate frictionless blood flow through blood vessels. Na(+) selectively shields these charges controlling surface electronegativity. The question was addressed whether the ambient Na(+) concentration controls RBC-EC interaction. Using atomic force microscopy (AFM) adhesion forces between ... Read more >>

Front Physiol (Frontiers in physiology)
[2015, 6:188]

Cited: 13 times

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Clathrin inhibitor Pitstop-2 disrupts the nuclear pore complex permeability barrier.

Ivan Liashkovich, Dzmitry Pasrednik, Valeria Prystopiuk, Gonzalo Rosso, Hans Oberleithner, Victor Shahin,

Existence of a selective nucleocytoplasmic permeability barrier is attributed to Phenylalanine-Glycine rich proteins (FG-nups) within the central channel of the nuclear pore complex (NPC). Limited understanding of the FG-nup structural arrangement hinders development of strategies directed at disrupting the NPC permeability barrier. In this report we explore an alternative approach ... Read more >>

Sci Rep (Scientific reports)
[2015, 5:9994]

Cited: 10 times

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Recharging red blood cell surface by hemodialysis.

Katrin Kliche, Ulrich Gerth, Hermann Pavenstädt, Hans Oberleithner,

<h4>Background</h4>Similar as in vascular endothelium the negatively charged glycocalyx of erythrocytes selectively buffers sodium. Loss of glycocalyx (i.e. loss of negative charges) leads to increased erythrocyte sodium sensitivity (ESS) quantified by a recently developed salt-blood-test (SBT). The hypothesis was tested whether a regular 4-hour hemodialysis (4h-HD) alters ESS.<h4>Methods</h4>In 38 patients ... Read more >>

Cell Physiol Biochem (Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology)
[2015, 35(3):1107-1115]

Cited: 1 time

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Salty stories.

Armin Kurtz, Hans Oberleithner,

Pflugers Arch (Pflugers Archiv : European journal of physiology)
[2015, 467(3):443]

Cited: 0 times

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Vascular glycocalyx sodium store – determinant of salt sensitivity?

Hans Oberleithner, Marianne Wilhelmi,

Smart mechanisms allow frictionless slipping of rather rigid erythrocytes (red blood cells, RBC) through narrow blood vessels. Nature solved this problem in an elegant way coating the moving object (RBC) and the tunnel wall (endothelium) by negative charges (glycocalyx). As long as these surfaces are intact, repulsive forces create a ... Read more >>

Blood Purif (Blood purification)
[2015, 39(1-3):7-10]

Cited: 4 times

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Damage of the endothelial glycocalyx in chronic kidney disease.

Jan-Sören Padberg, Anne Wiesinger, Giovana Seno di Marco, Stefan Reuter, Alexander Grabner, Dominik Kentrup, Alexander Lukasz, Hans Oberleithner, Hermann Pavenstädt, Marcus Brand, Philipp Kümpers,

<h4>Background and objectives</h4>The endothelial glycocalyx (eGC), a mesh of anionic biopolymers covering the luminal surface of endothelial cells, is considered as an intravascular compartment that protects the vessel wall against pathogenic insults in cardiovascular disease. We hypothesized that chronic kidney disease (CKD) is associated with reduced eGC integrity and subsequent ... Read more >>

Atherosclerosis (Atherosclerosis)
[2014, 234(2):335-343]

Cited: 74 times

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Nanomechanics of vascular endothelium.

Johannes Fels, Pia Jeggle, Ivan Liashkovich, Wladimir Peters, Hans Oberleithner,

The mechanical characteristics of endothelial cells reveal four distinct compartments, namely glycocalyx, cell cortex, cytoplasm and nucleus. There is accumulating evidence that endothelial nanomechanics of these individual compartments control vascular physiology. Depending on protein composition, filament formation and interaction with cross-linker proteins, these four compartments determine endothelial stiffness. Structural organization ... Read more >>

Cell Tissue Res (Cell and tissue research)
[2014, 355(3):727-737]

Cited: 40 times

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Sodium selective erythrocyte glycocalyx and salt sensitivity in man.

Hans Oberleithner,

Negatively charged surfaces of erythrocytes (RBC) reflect properties of the endothelial glycocalyx. Plasma electrolytes counteract these charges and thus control the repulsive forces between RBC and endothelium. Although Na(+) is supposed to exert a rather high affinity to the RBC surface, a direct comparison between Na(+) and K(+) in counteracting ... Read more >>

Pflugers Arch (Pflugers Archiv : European journal of physiology)
[2015, 467(6):1319-1325]

Cited: 8 times

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Endothelial sodium channels trigger endothelial salt sensitivity with aging.

Moritz Paar, Hermann Pavenstädt, Kristina Kusche-Vihrog, Verena Drüppel, Hans Oberleithner, Katrin Kliche,

The epithelial sodium channel is also expressed in vascular endothelium (endothelial sodium channel [EnNaC]). Depending on ambient sodium concentration, EnNaC is associated with mechanical stiffening of the endothelial cell cortex, leading to endothelial dysfunction. Because the incidence of both salt sensitivity and endothelial dysfunction increases with age, we investigated the ... Read more >>

Hypertension (Hypertension (Dallas, Tex. : 1979))
[2014, 64(2):391-396]

Cited: 25 times

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Feedforward activation of endothelial ENaC by high sodium.

Stefanie Korte, Alexandra S Sträter, Verena Drüppel, Hans Oberleithner, Pia Jeggle, Claudia Grossmann, Manfred Fobker, Jerzy-Roch Nofer, Eva Brand, Kristina Kusche-Vihrog,

Kidney epithelial sodium channels (ENaCs) are known to be inactivated by high sodium concentrations (feedback inhibition). Recently, the endothelial sodium channel (EnNaC) was identified to control the nanomechanical properties of the endothelium. EnNaC-dependent endothelial stiffening reduces the release of nitric oxide, the hallmark of endothelial dysfunction. To study the regulatory ... Read more >>

FASEB J (FASEB journal : official publication of the Federation of American Societies for Experimental Biology)
[2014, 28(9):4015-4025]

Cited: 22 times

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Blood pressure and amiloride-sensitive sodium channels in vascular and renal cells.

David G Warnock, Kristina Kusche-Vihrog, Antoine Tarjus, Shaohu Sheng, Hans Oberleithner, Thomas R Kleyman, Frederic Jaisser,

Sodium transport in the distal nephron is mediated by epithelial sodium channel activity. Proteolytic processing of external domains and inhibition with increased sodium concentrations are important regulatory features of epithelial sodium channel complexes expressed in the distal nephron. By contrast, sodium channels expressed in the vascular system are activated by ... Read more >>

Nat Rev Nephrol (Nature reviews. Nephrology)
[2014, 10(3):146-157]

Cited: 55 times

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Fixed endothelial cells exhibit physiologically relevant nanomechanics of the cortical actin web.

Kai Bodo Grimm, Hans Oberleithner, Johannes Fels,

It has been unknown whether cells retain their mechanical properties after fixation. Therefore, this study was designed to compare the stiffness properties of the cell cortex (the 50-100 nm thick zone below the plasma membrane) before and after fixation. Atomic force microscopy was used to acquire force indentation curves from ... Read more >>

Nanotechnology (Nanotechnology)
[2014, 25(21):215101]

Cited: 16 times

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