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Author Han Ting Chou

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Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains.

Samiksha Kaul, Han Ting Chou, Seleipiri Charles, Guillaume Aubry, Hang Lu, Annalise B Paaby,

Wild <i>C. elegans</i> strains harbor natural variation in developmental pathways, but investigating these differences requires precise and well-powered phenotyping methods. Here we employ a microfluidics platform for single-molecule FISH to simultaneously visualize the transcripts of three genes in embryos of two distinct strains. We capture transcripts at high resolution by ... Read more >>

MicroPubl Biol (microPublication biology)
[2022, 2022:]

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Diversification of small RNA pathways underlies germline RNAi incompetence in wild C. elegans strains

Han Ting Chou, Francisco Valencia, Jacqueline Alexander, Avery Davis Bell, Diptodip Deb, Daniel Pollard, Annalise Paaby,

<h4>ABSTRACT</h4> The discovery that experimental delivery of dsRNA can induce gene silencing at target genes revolutionized genetics research, by both uncovering essential biological processes and creating new tools for developmental geneticists. However, the efficacy of exogenous RNAi varies dramatically within the C. elegans population, raising questions about our understanding of ... Read more >>

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