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Molecular cloning and characterization of FADD from the grass carp (Ctenopharyngodon idellus) in response to bacterial infection

Zhijie Lu, Meizhen Tang, Yanan Li, Fei Shi, Fanbin Zhan, Menglan Zhang, Lijuan Zhao, Jun Li, Li Lin, Zhendong Qin,

Fas-associated protein with Death Domain (FADD) is closely associated with cell death and plays a key role in hematopoiesis, cell cycle regulation, embryogenesis, and particularly, innate immune signaling. However, the mechanisms underlying the apoptotic and immune responses against bacterial infection associated with FADD in teleost fish have yet to be ... Read more >>

Aquaculture (Aquaculture (Amsterdam, Netherlands))
[2021, 542:Not Available]

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Immune function of cytosolic manganese superoxide dismutase from Macrobrachium rosenbergii in response to bacterial infection

Yanan Li, Fanbin Zhan, Fenglin Li, Zhijie Lu, Fei Shi, Zizheng Xu, Youcheng Yang, Lijuan Zhao, Zhendong Qin, Li Lin,

An excess of reactive oxygen species (ROS) usually disrupts the balance of the antioxidant system and impairs the homeostasis of cells, resulting in serious oxidative damage. Therefore, a better understanding of how the immune system protects itself against such excessive oxidative stress is crucial. SODs play an indispensable role in ... Read more >>

Aquaculture (Aquaculture (Amsterdam, Netherlands))
[2021, 541:Not Available]

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Hemeprotein amplifies the innate immune receptors of Ctenopharyngodon idellus kidney cells through NF-κB- and MAPK-dependent reactive oxygen species generation.

Zhijie Lu, Meizhen Tang, Menglan Zhang, Yanan Li, Fei Shi, Fanbin Zhan, Lijuan Zhao, Jun Li, Li Lin, Zhendong Qin,

Infectious bacterial and viral diseases that cause hemolysis are considered life-threatening to grass carp (Ctenopharyngodon idellus), which is a species used in aquaculture worldwide. After heme and hemeproteins (Hb) are released as a result of hemolysis, the effect of excess Hb and heme on tissues remains to be characterized. To ... Read more >>

Dev Comp Immunol (Developmental and comparative immunology)
[2021, :104207]

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Multi-discriminator adversarial convolutional network for nerve fiber segmentation in confocal corneal microscopy images.

Changqing Yang, Xinxin Zhou, Weifang Zhu, Dehui Xiang, Zhongyue Chen, Jin Yuan, Xinjian Chen, Fei Shi,

Quantitative measurements of corneal sub-basal nerves are biomarkers for many ocular surface disorders, and are also important for early diagnosis and assessment of progression of neurodegenerative diseases. This paper aims to develop an automatic method for nerve fiber segmentation from in vivo corneal confocal microscopy (CCM) images, which is fundamental ... Read more >>

IEEE J Biomed Health Inform (IEEE journal of biomedical and health informatics)
[2021, PP:]

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Increased levels of serum IL-17 and induced sputum neutrophil percentage are associated with severe early-onset asthma in adults.

Dandan Chen, Yu Zhang, Can Yao, Binbin Li, Sinian Li, Wenwen Liu, Rongchang Chen, Fei Shi,

<h4>Background</h4>Differences between adult patients with severe early-onset and late-onset asthma have not been well studied.<h4>Objectives</h4>To determine the phenotypic distinction regarding age at onset in patients with severe asthma.<h4>Methods</h4>The present study enrolled thirty-two patients with severe early-onset (onset age < 12 years) asthma and thirty-two patients with severe late-onset (onset age > 12 years) asthma. Severe asthma ... Read more >>

Allergy Asthma Clin Immunol (Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology)
[2021, 17(1):64]

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Alginate oligosaccharide improves fat metabolism and antioxidant capacity in the liver of grass carp (Ctenopharyngodon idellus)

Minxuan Yang, Zhijie Lu, Fenglin Li, Fei Shi, Fanbin Zhan, Yulei Zhang, Lijuan Zhao, Yanan Li, Jun Li, Li Lin, Zhendong Qin,

The aim of the current study was to investigate the potentially beneficial effects of alginate oligosaccharide (AOS) on the modulation of growth, fatty acid metabolism, and antioxidant capacity in grass carp (Ctenopharyngodon idellus), and the fundamental mechanisms involved. AOS enhanced the growth performance of grass carp in a dose-dependent manner. ... Read more >>

Aquaculture (Aquaculture (Amsterdam, Netherlands))
[2021, 540:Not Available]

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Expression and functional analysis of the BCL2-Associated agonist of cell death (BAD) gene in grass carp (Ctenopharyngodon idella) during bacterial infection.

Zhijie Lu, Meizhen Tang, Menglan Zhang, Yanan Li, Fei Shi, Fanbin Zhan, Lijuan Zhao, Jun Li, Li Lin, Zhendong Qin,

The BCL2-associated agonist of cell death protein is a key participant in apoptosis dependent on mitochondria and in disease progression that involves the regulation of cell death, such as tumorigenesis, diabetes, sepsis shock, and epilepsy. Nevertheless, the mechanisms underlying the immune responses to teleost BAD bacterial infection and mitochondrial-dependent apoptosis ... Read more >>

Dev Comp Immunol (Developmental and comparative immunology)
[2021, 123:104160]

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Autophagy was activated against the damages of placentas caused by nano-copper oral exposure.

Min Kang, Jie Luo, Ling Zhao, Fei Shi, Gang Ye, Xiaoli He, Suqi Hao, Dan Yang, Helin Chen, Hongrui Guo, Yinglun Li, Huaqiao Tang,

Nano-copper (nano-Cu) is widely used in the pharmaceutical field as well as a feed additive for animals owing to its unique physicochemical characteristics and bioactivities. In our previous study, nano-Cu was found to hamper fetal development; however, the toxicity of nano-Cu and its effects in placental function have not been ... Read more >>

Ecotoxicol Environ Saf (Ecotoxicology and environmental safety)
[2021, 220:112364]

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EGCG-Mediated Potential Inhibition of Biofilm Development and Quorum Sensing in Pseudomonas aeruginosa.

Suqi Hao, Dan Yang, Ling Zhao, Fei Shi, Gang Ye, Hualin Fu, Juchun Lin, Hongrui Guo, Ran He, Jianlong Li, Hongwei Chen, Muhammad Faraz Khan, Yinglun Li, Huaqiao Tang,

<i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>), one of the dangerous multidrug resistance pathogens, orchestrates virulence factors production through quorum sensing (QS). Since the exploration of QS inhibitors, targeting virulence to circumvent bacterial pathogenesis without causing significant growth inhibition is a promising approach to treat <i>P. aeruginosa</i> infections. The present study has evaluated ... Read more >>

Int J Mol Sci (International journal of molecular sciences)
[2021, 22(9):]

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The feasibility of using Hcy, CRP, and Cys-C to analyze AMI patients' disease conditions and prognoses.

Na Zhang, Fei Shi, Hao Liang, Hong Wang,

<h4>Objective</h4>This study was conducted to investigate the feasibility of using the serum homocysteine (Hcy), C-reactive protein (CRP), and cystatin C (Cys-C) levels to evaluate the conditions and prognoses of acute myocardial infarction (AMI) patients.<h4>Methods</h4>One hundred and twenty patients with AMI were enrolled as an observation group (the AMI group). Eighty ... Read more >>

Am J Transl Res (American journal of translational research)
[2021, 13(4):2724-2730]

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Speckle Noise Reduction for OCT Images based on Image Style Transfer and Conditional GAN.

Yi Zhou, Kai Yu, Meng Wang, Yuhui Ma, Yuanyuan Peng, Zhongyue Chen, Weifang Zhu, Fei Shi, Xinjian Chen,

Raw optical coherence tomography (OCT) images typically are of low quality because speckle noise blurs retinal structures, severely compromising visual quality and degrading performances of subsequent image analysis tasks. In our previous study, we have developed a Conditional Generative Adversarial Network (cGAN) for speckle noise removal in OCT images collected ... Read more >>

IEEE J Biomed Health Inform (IEEE journal of biomedical and health informatics)
[2021, PP:]

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Nrf2 modulates host defense during antibacterial immunity response in grass carp (Ctenopharyngodon idellus)

Yanan Li, Zhijie Lu, Fanbin Zhan, Minxuan Yang, Lijuan Zhao, Fei Shi, Jun Li, Li Lin, Zhendong Qin,

Excessive production of reactive oxygen species (ROS) usually breaks the body's homeostasis and results in oxidative damage. It has been reported that transcription factor NF-E2-related factor 2 (Nrf2) can regulate redox balance for host defense, which activates diverse cyto-protective genes to maintain intracellular homoeostasis during pathogen invasion. Several studies of ... Read more >>

Aquaculture (Aquaculture (Amsterdam, Netherlands))
[2021, 536:Not Available]

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Semi-Supervised Capsule cGAN for Speckle Noise Reduction in Retinal OCT Images.

Meng Wang, Weifang Zhu, Kai Yu, Zhongyue Chen, Fei Shi, Yi Zhou, Yuhui Ma, Yuanyuan Peng, Dengsen Bao, Shuanglang Feng, Lei Ye, Dehui Xiang, Xinjian Chen,

Speckle noise is the main cause of poor optical coherence tomography (OCT) image quality. Convolutional neural networks (CNNs) have shown remarkable performances for speckle noise reduction. However, speckle noise denoising still meets great challenges because the deep learning-based methods need a large amount of labeled data whose acquisition is time-consuming ... Read more >>

IEEE Trans Med Imaging (IEEE transactions on medical imaging)
[2021, 40(4):1168-1183]

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The small protein MafG plays a critical role in MC3T3-E1 cell apoptosis induced by simulated microgravity and radiation.

Honghui Wang, Jingjing Dong, Gaozhi Li, Yingjun Tan, Hai Zhao, Lijun Zhang, Yixuan Wang, Zebing Hu, Xinsheng Cao, Fei Shi, Shu Zhang,

Microgravity and radiation exposure-induced bone damage is one of the most significant alterations in astronauts after long-term spaceflight. However, the underlying mechanism is still largely unknown. Recent ground-based simulation studies have suggested that this impairment is likely mediated by increased production of reactive oxygen species (ROS) during spaceflight. The small ... Read more >>

Biochem Biophys Res Commun (Biochemical and biophysical research communications)
[2021, 555:175-181]

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Prognostic value of peripheral blood inflammatory cell subsets in patients with acute coronary syndrome undergoing percutaneous coronary intervention.

Xiuxin Gao, Yixiang Liu, Yanan Tian, Chongyou Rao, Fei Shi, Haiwei Bu, Jingyi Liu, Ying Zhang, Weichao Shan, Zhenjiang Ding, Lixian Sun,

<h4>Objective</h4>This study aimed to investigate the predictive value of inflammatory cells in peripheral blood on the prognosis of patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI).<h4>Methods</h4>Patients (n=1558) were consecutively enrolled and the median follow-up was 1142 days. Patients were divided into the major adverse cardiac events (MACE) ... Read more >>

J Int Med Res (The Journal of international medical research)
[2021, 49(4):3000605211010059]

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Gentamycin irrigation significantly reduces 28-day surgical site infection after emergency neurosurgery.

Jin Wang, Ying Yang, Wen Lv, Shihai Xu, Shanshan Mei, Fei Shi, Aijun Shan,

<h4>Objective</h4>This study aims to evaluate the effectiveness of gentamycin irrigation in preventing postoperative surgical site infection (SSI) in emergency neurosurgical procedures.<h4>Methods</h4>A total of 518 consecutive emergency neurosurgeries, including craniotomies, endoscopic and burr hole procedures were reviewed retrospectively. Patients received either only normal saline (NS) irrigation or NS irrigation with gentamycin ... Read more >>

Br J Neurosurg (British journal of neurosurgery)
[2021, :1-5]

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Work experience of triage nurses in emergency departments during the prevalence of COVID-19

Shihai Xu, Qiaohong Yang, Manying Xie, Jin Wang, Aijun Shan, Fei Shi,

<h4>Objective</h4> In December 2019, the global outbreak of Corona Virus Disease 2019 (COVID 19) was reported. As of March 8, 2020, more than 90,000 cases were reported worldwide, resulting in a shortage of global medical resources. The purpose of this study was to understand the working experience of triage nurses ... Read more >>

Int Emerg Nurs (International emergency nursing)
[2021, :]

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Induction of apoptosis in SSN-1cells by Snakehead Fish Vesiculovirus (SHVV) via Matrix protein dependent intrinsic pathway.

Abeer M Hegazy, Nan Chen, Hanzuo Lin, Sarath Babu V, Feng Li, Youcheng Yang, Zhendong Qin, Fei Shi, Jun Li, Li Lin,

An increasing important area in immunology is the process cell death mechanism, enabling the immune system triggered thru extrinsic or intrinsic signals to effectively remove unwanted or virus infected cells called apoptosis. A recently isolated infectious Snakehead fish vesiculovirus (SHVV), comprising negative strand RNA and encoded viral matrix (M) proteins, ... Read more >>

Fish Shellfish Immunol (Fish & shellfish immunology)
[2021, 113:24-34]

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BDKRB2 is a novel EMT-related biomarker and predicts poor survival in glioma.

Ying Yang, Jin Wang, Fei Shi, Aijun Shan, Shihai Xu, Wen Lv,

Bradykinin receptor B2 (BDKRB2) has been reported as an oncogene in several malignancies. In glioma, the role of BDKRB2 remains unknown. This study aimed at investigating its clinical significance and biological function in glioma at the transcriptional level. We selected 301 glioma patients with microarray data from CGGA database and ... Read more >>

Aging (Albany NY) (Aging)
[2021, 13(5):7499-7516]

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Work experience of triage nurses in emergency departments during the prevalence of COVID-19.

Shihai Xu, Qiaohong Yang, Manying Xie, Jin Wang, Aijun Shan, Fei Shi,

<h4>Objective</h4>In December 2019, the global outbreak of Corona Virus Disease 2019 (COVID 19) was reported. As of March 8, 2020, more than 90,000 cases were reported worldwide, resulting in a shortage of global medical resources. The purpose of this study was to understand the working experience of triage nurses in ... Read more >>

Int Emerg Nurs (International emergency nursing)
[2021, 56:101003]

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Elevated Serum Levels of Herpes Virus Entry Mediator and IL-5 in Cough Variant Asthma Versus Classic Asthma Patients

Can Yao, Zhong Yang, Dandan Chen, Wei Fang, Yu Zhang, Binbin Li, Sinian Li, Rongchang Chen, Fei Shi,

<h4>Background: </h4> Cough variant asthma (CVA) is a special phenotype of asthma. The precise inflammatory features of patients with CVA, however, is still unclear. We aimed to elucidate the differential inflammatory features in patients with CVA in contrast to patients with classic asthma (CA). <h4>Methods: </h4> A total of 68 ... Read more >>

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Astragalus polysaccharides mediate the immune response and intestinal microbiota in grass carp (Ctenopharyngodon idellus)

Fei Shi, Zhijie Lu, Minxuan Yang, Feng Li, Fanbin Zhan, Lijuan Zhao, Yanan Li, Qingqing Li, Jiangtao Li, Jun Li, Li Lin, Zhendong Qin,

The immunomodulatory effect of Astragalus polysaccharides (APS) has been revealed in aquatic animals. However, the fundamental mechanisms in grass carp (Ctenopharyngodon idellus) are not well characterized. This research has explored the impacts of APS on immune response, gut microbes and transcriptome profiles of grass carp. The eight week feeding trial ... Read more >>

Aquaculture (Aquaculture (Amsterdam, Netherlands))
[2021, 534:Not Available]

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Combining Blood-Based Biomarkers to Predict Mortality of Sepsis at Arrival at the Emergency Department.

Yinjing Xie, Binbin Li, Ying Lin, Fei Shi, Weibu Chen, Wenyuan Wu, Wenjia Zhang, Yun Fei, Shiqing Zou, Can Yao,

BACKGROUND Our aim was to determine a useful combination of blood biomarkers that can predict 28-day mortality of sepsis upon arrival at the Emergency Department (ED). MATERIAL AND METHODS Based on Sepsis-3.0, 90 sepsis patients were enrolled and divided into survivor and nonsurvivor groups with day 28 as the study ... Read more >>

Med Sci Monit (Medical science monitor : international medical journal of experimental and clinical research)
[2021, 27:e929527]

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Optimized combination of circulating biomarkers as predictors of prognosis in AECOPD patients complicated with Heart Failure.

Can Yao, Lingwei Wang, Fei Shi, Rongchang Chen, Binbin Li, Wenwen Liu, Mengjie Feng, Sinian Li,

<b>Background:</b> Systematic inflammation, nutritional status, and cardiovascular function have been associated with the outcomes of acute exacerbation of chronic obstructive pulmonary disease (AECOPD) patients with heart failure (HF). However, the value of their relevant biomarkers in predicting mortality has not been well defined yet. We aimed to investigate the prognostic ... Read more >>

Int J Med Sci (International journal of medical sciences)
[2021, 18(7):1592-1599]

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Umbilical Cord Mesenchymal Stem Cells for the Treatment of Atherosclerosis in a Mouse Model: A Randomized Controlled Trial

Yijia Song, Gaomiyang Liu, Fei Shi, Xiaochun Jiang, Jufen Liu, Xuejuan Zhang, Qingkeng Lin, Falian He, Zian Li, Jie He, Guangping Ruan, Rongqing Pang, Xinghua Pan, Xiangqing Zhu,

<h4>Background: </h4> The purpose of this study was to determine the efficacy of umbilical cord mesenchymal stem cell (UCMSC) transplantation in a mouse atherosclerosis (AS) model and explore its potential antioxidative stress and lipid metabolism regulatory effects.MethodsA mouse AS model was established by feeding ApoE -/- mice a high-fat diet, ... Read more >>

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