Full Text Journal Articles by
Author David Longbottom

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Defining immune correlates during latent and active chlamydial infection in sheep.

Sean R Wattegedera, Morag Livingstone, Stephen Maley, Mara Rocchi, Susan Lee, Yvonne Pang, Nick M Wheelhouse, Kevin Aitchison, Javier Palarea-Albaladejo, David Buxton, David Longbottom, Gary Entrican,

Ovine enzootic abortion (OEA) caused by the obligate intracellular bacterial pathogen Chlamydia abortus (C. abortus), is an endemic disease in most sheep-rearing countries worldwide. Following infection, C. abortus establishes a complex host-pathogen interaction with a latent phase in non-pregnant sheep followed by an active disease phase in the placenta during ... Read more >>

Vet. Res. (Veterinary research)
[2020, 51(1):75]

Cited: 0 times

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Complete Genome Sequence of Chlamydia avium PV 4360/2, Isolated from a Feral Pigeon in Italy.

Anna Maria Floriano, Sara Rigamonti, Francesco Comandatore, Erika Scaltriti, David Longbottom, Morag Livingstone, Karine Laroucau, Alessandra Gaffuri, Stefano Pongolini, Simone Magnino, Nadia Vicari,

We report here the whole-genome sequence of a Chlamydia avium isolate recovered from a feral pigeon in 1999 in Italy. Only one complete genome of a C. avium strain has been published so far. Future comparative analyses could provide valuable insights on the genomic evolution of the pathogen. ... Read more >>

Microbiol Resour Announc (Microbiology resource announcements)
[2020, 9(16):]

Cited: 0 times

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Proteomic characterisation of the Chlamydia abortus outer membrane complex (COMC) using combined rapid monolithic column liquid chromatography and fast MS/MS scanning.

David Longbottom, Morag Livingstone, Kevin D Aitchison, Lisa Imrie, Erin Manson, Nicholas Wheelhouse, Neil F Inglis,

Data are presented on the identification and partial characterisation of proteins comprising the chlamydial outer membrane complex (COMC) fraction of Chlamydia abortus (C. abortus)-the aetiological agent of ovine enzootic abortion. Inoculation with the COMC fraction is known to be highly effective in protecting sheep against experimental challenge and its constituent ... Read more >>

PLoS ONE (PloS one)
[2019, 14(10):e0224070]

Cited: 0 times

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Genomic evidence that the live Chlamydia abortus vaccine strain 1B is not attenuated and has the potential to cause disease.

David Longbottom, Michelle Sait, Morag Livingstone, Karine Laroucau, Konrad Sachse, Simon R Harris, Nicholas R Thomson, Helena M B Seth-Smith,

The live, temperature-attenuated vaccine strain 1B of Chlamydia abortus, the aetiological agent of ovine enzootic abortion (OEA), has been implicated in cases of vaccine breakdown. The aim of this study was to understand the nature of this attenuation through sequencing of the vaccine parent strain (AB7) and the derived mutant ... Read more >>

Vaccine (Vaccine)
[2018, 36(25):3593-3598]

Cited: 1 time

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Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain.

Martina Velasova, Angela Damaso, Bhagyalakshmi Chengat Prakashbabu, Jenny Gibbons, Nick Wheelhouse, David Longbottom, Steven Van Winden, Martin Green, Javier Guitian,

To implement appropriate and effective disease control programs at the national level, up-to-date and unbiased information on disease frequency is needed. The aim of this study was to estimate the prevalence of selected endemic infectious diseases in the population of dairy herds in Great Britain. Bulk milk tank (BMT) samples ... Read more >>

J. Dairy Sci. (Journal of dairy science)
[2017, 100(11):9215-9233]

Cited: 8 times

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Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS.

Morag Livingstone, Nicholas Wheelhouse, Hannah Ensor, Mara Rocchi, Stephen Maley, Kevin Aitchison, Sean Wattegedera, Kim Wilson, Michelle Sait, Victoria Siarkou, Evangelia Vretou, Gary Entrican, Mark Dagleish, David Longbottom,

This study investigated the pathogenesis of two variant strains (LLG and POS) of Chlamydia abortus, in comparison to a typical wild-type strain (S26/3) which is known to be responsible for late term abortion in small ruminants. Challenge with the three strains at mid-gestation resulted in similar pregnancy outcomes, with abortion ... Read more >>

PLoS ONE (PloS one)
[2017, 12(5):e0177653]

Cited: 2 times

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Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila.

Nicholas Wheelhouse, Allen Flockhart, Kevin Aitchison, Morag Livingstone, Jeanie Finlayson, Virginie Flachon, Eric Sellal, Mark P Dagleish, David Longbottom,

Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in cattle. Despite an increasing number of observational studies linking W. chondrophila infection to cattle abortion, little direct experimental evidence exists. Given this paucity of direct ... Read more >>

Sci Rep (Scientific reports)
[2016, 6:37150]

Cited: 3 times

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Analysis of Humoral Immune Responses to Surface and Virulence-Associated Chlamydia abortus Proteins in Ovine and Human Abortions by Use of a Newly Developed Line Immunoassay.

Jürgen Benjamin Hagemann, Ulrike Simnacher, David Longbottom, Morag Livingstone, Julia Maile, Erwin Soutschek, Gernot Walder, Katharina Boden, Konrad Sachse, Andreas Essig,

The obligate intracellular bacterium Chlamydia abortus is the causative agent of enzootic abortion of ewes and poses a significant zoonotic risk for pregnant women. Using proteomic analysis and gene expression library screening in a previous project, we identified potential virulence factors and candidates for serodiagnosis, of which nine were scrutinized ... Read more >>

J. Clin. Microbiol. (Journal of clinical microbiology)
[2016, 54(7):1883-1890]

Cited: 6 times

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Ovine trophoblasts express cathelicidin host defence peptide in response to infection.

Christopher Coyle, Nick Wheelhouse, Maxime Jacques, David Longbottom, Pavel Svoboda, Jan Pohl, W Colin Duncan, Michael T Rae, Peter G Barlow,

Cationic host defence peptides (CHDP; also known as antimicrobial peptides) are key components of the immune response in the female reproductive tract. The role of the placental trophoblast in ovine host defence remains poorly understood. This study characterises expression of genes for cathelicidin and defensin peptides in primary ovine placental ... Read more >>

J. Reprod. Immunol. (Journal of reproductive immunology)
[2016, 117:10-16]

Cited: 1 time

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MAPK Activation Is Essential for Waddlia chondrophila Induced CXCL8 Expression in Human Epithelial Cells.

Skye Storrie, David Longbottom, Peter G Barlow, Nick Wheelhouse,

BACKGROUND:Waddlia chondrophila (W. chondrophila) is an emerging agent of respiratory and reproductive disease in humans and cattle. The organism is a member of the order Chlamydiales, and shares many similarities at the genome level and in growth studies with other well-characterised zoonotic chlamydial agents, such as Chlamydia abortus (C. abortus). ... Read more >>

PLoS ONE (PloS one)
[2016, 11(3):e0152193]

Cited: 2 times

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Improving the molecular diagnosis of Chlamydia psittaci and Chlamydia abortus infection with a species-specific duplex real-time PCR.

Onya Opota, Katia Jaton, James Branley, Daisy Vanrompay, Veronique Erard, Nicole Borel, David Longbottom, Gilbert Greub,

Chlamydia psittaci and Chlamydia abortus are closely related intracellular bacteria exhibiting different tissue tropism that may cause severe but distinct infection in humans. C. psittaci causes psittacosis, a respiratory zoonotic infection transmitted by birds. C. abortus is an abortigenic agent in small ruminants, which can also colonize the human placenta ... Read more >>

J. Med. Microbiol. (Journal of medical microbiology)
[2015, 64(10):1174-1185]

Cited: 8 times

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Diversification and Distribution of Ruminant Chlamydia abortus Clones Assessed by MLST and MLVA.

Victoria I Siarkou, Fabien Vorimore, Nadia Vicari, Simone Magnino, Annie Rodolakis, Yvonne Pannekoek, Konrad Sachse, David Longbottom, Karine Laroucau,

Chlamydia abortus, an obligate intracellular bacterium, is the most common infectious cause of abortion in small ruminants worldwide and has zoonotic potential. We applied multilocus sequence typing (MLST) together with multiple-locus variable-number tandem repeat analysis (MLVA) to genotype 94 ruminant C. abortus strains, field isolates and samples collected from 1950 ... Read more >>

PLoS ONE (PloS one)
[2015, 10(5):e0126433]

Cited: 4 times

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Abortion in flocks vaccinated against enzootic abortion.

Morag Livingstone, Kevin Aitchison, David Longbottom,

Vet. Rec. (The Veterinary record)
[2014, 174(24):613-614]

Cited: 2 times

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Waddlia chondrophila infects and multiplies in ovine trophoblast cells stimulating an inflammatory immune response.

Nick Wheelhouse, Christopher Coyle, Peter G Barlow, Stephen Mitchell, Gilbert Greub, Tim Baszler, Mick T Rae, David Longbottom,

Waddlia chondrophila (W. chondrophila) is an emerging abortifacient organism which has been identified in the placentae of humans and cattle. The organism is a member of the order Chlamydiales, and shares many similarities at the genome level and in growth studies with other well-characterised zoonotic chlamydial abortifacients, such as Chlamydia ... Read more >>

PLoS ONE (PloS one)
[2014, 9(7):e102386]

Cited: 7 times

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Chlamydia in cases of bovine abortion in Ireland.

Nicholas Wheelhouse, David Longbottom, Jim O'Donovan,

Vet. Rec. (The Veterinary record)
[2014, 174(22):560-561]

Cited: 2 times

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Genome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomes.

Michelle Sait, Morag Livingstone, Ewan M Clark, Nick Wheelhouse, Lucy Spalding, Bryan Markey, Simone Magnino, Frederick A Lainson, Garry S A Myers, David Longbottom,

Chlamydia pecorum is the causative agent of a number of acute diseases, but most often causes persistent, subclinical infection in ruminants, swine and birds. In this study, the genome sequences of three C. pecorum strains isolated from the faeces of a sheep with inapparent enteric infection (strain W73), from the ... Read more >>

BMC Genomics (BMC genomics)
[2014, 15:23]

Cited: 18 times

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Profiling antibody responses to infections by Chlamydia abortus enables identification of potential virulence factors and candidates for serodiagnosis.

Vera Forsbach-Birk, Corinna Foddis, Ulrike Simnacher, Max Wilkat, David Longbottom, Gernot Walder, Christiane Benesch, Martin Ganter, Konrad Sachse, Andreas Essig,

Enzootic abortion of ewes (EAE) due to infection with the obligate intracellular pathogen Chlamydia (C.) abortus is an important zoonosis leading to considerable economic loss to agriculture worldwide. The pathogen can be transmitted to humans and may lead to serious infection in pregnant women. Knowledge about epidemiology, clinical course and ... Read more >>

PLoS ONE (PloS one)
[2013, 8(11):e80310]

Cited: 10 times

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Intranasal infection with Chlamydia abortus induces dose-dependent latency and abortion in sheep.

David Longbottom, Morag Livingstone, Stephen Maley, Arjan van der Zon, Mara Rocchi, Kim Wilson, Nicholas Wheelhouse, Mark Dagleish, Kevin Aitchison, Sean Wattegedera, Mintu Nath, Gary Entrican, David Buxton,

Latency is a key feature of the animal pathogen Chlamydia abortus, where infection remains inapparent in the non-pregnant animal and only becomes evident during a subsequent pregnancy. Often the first sign that an animal is infected is abortion occurring late in gestation. Despite this, little is understood of the underlying ... Read more >>

PLoS ONE (PloS one)
[2013, 8(2):e57950]

Cited: 23 times

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Processing of Chlamydia abortus polymorphic membrane protein 18D during the chlamydial developmental cycle.

Nick M Wheelhouse, Michelle Sait, Kevin Aitchison, Morag Livingstone, Frank Wright, Kevin McLean, Neil F Inglis, David G E Smith, David Longbottom,

Chlamydia possess a unique family of autotransporter proteins known as the Polymorphic membrane proteins (Pmps). While the total number of pmp genes varies between Chlamydia species, all encode a single pmpD gene. In both Chlamydia trachomatis (C. trachomatis) and C. pneumoniae, the PmpD protein is proteolytically cleaved on the cell ... Read more >>

PLoS ONE (PloS one)
[2012, 7(11):e49190]

Cited: 11 times

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Genome Sequence of Chlamydia psittaci Strain 01DC12 Originating from Swine.

Helena M B Seth-Smith, Michelle Sait, Konrad Sachse, Wolfgang Gaede, David Longbottom, Nicholas R Thomson,

Chlamydia psittaci is the etiological agent of psittacosis and is a zoonotic pathogen infecting birds and a variety of mammalian hosts. Here we report the genome sequence of the porcine strain 01DC12 which is representative of a novel clade of C. psittaci belonging to ompA genotype E. ... Read more >>

Genome Announc (Genome announcements)
[2013, 1(1):]

Cited: 2 times

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Genome Sequence of Lawsonia intracellularis Strain N343, Isolated from a Sow with Hemorrhagic Proliferative Enteropathy.

Michelle Sait, Kevin Aitchison, Nick Wheelhouse, Kim Wilson, F Alex Lainson, David Longbottom, David G E Smith,

Lawsonia intracellularis is the etiological agent of proliferative enteropathy (PE), causing mild or acute hemorrhagic diarrhea in infected animals. Here we report the genome sequence of strain N343, isolated from a sow that died of hemorrhagic PE. N343 contains 24 single nucleotide polymorphisms and 90 indels compared to the reference ... Read more >>

Genome Announc (Genome announcements)
[2013, 1(1):]

Cited: 4 times

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Chlamydia in cases of cattle pneumonia in Scotland.

Nicholas Wheelhouse, David Longbottom, Kim Willoughby,

Vet. Rec. (The Veterinary record)
[2013, 172(4):110]

Cited: 6 times

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Evaluation of the impact and control of enzootic abortion of ewes.

David Longbottom, Gary Entrican, Nicholas Wheelhouse, Helen Brough, Catherine Milne,

Despite the availability of effective management and treatment strategies, Chlamydia abortus remains the single most frequently diagnosed cause of infectious ovine abortion (enzootic abortion of ewes, EAE) in the UK and one of the most significant causes of lamb mortality world-wide. In 2007, a survey of UK farmers, veterinarians and ... Read more >>

Vet. J. (Veterinary journal (London, England : 1997))
[2013, 195(2):257-259]

Cited: 6 times

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Expression patterns of five polymorphic membrane proteins during the Chlamydia abortus developmental cycle.

Nick Wheelhouse, Michelle Sait, Kim Wilson, Kevin Aitchison, Kevin McLean, David G E Smith, David Longbottom,

It has been suggested that polymorphic membrane proteins (Pmps) belonging to the Type V autotransporter protein family play an important role in the pathogenesis of Chlamydia abortus (C. abortus; formerly Chlamydophila abortus) infection. In a previous study we demonstrated the expression of all the pmps at the transcriptional level. The ... Read more >>

Vet. Microbiol. (Veterinary microbiology)
[2012, 160(3-4):525-529]

Cited: 6 times

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New challenges for vaccination to prevent chlamydial abortion in sheep.

Gary Entrican, Nick Wheelhouse, Sean R Wattegedera, David Longbottom,

Ovine enzootic abortion (OEA) is caused by the obligate intracellular Gram-negative bacterium Chlamydia abortus. OEA remains a common cause of infectious abortion in many sheep-rearing countries despite the existence of commercially available vaccines that protect against the disease. There are a number of confounding factors that influence the uptake and ... Read more >>

Comp. Immunol. Microbiol. Infect. Dis. (Comparative immunology, microbiology and infectious diseases)
[2012, 35(3):271-276]

Cited: 8 times

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