Full Text Journal Articles by
Author David F Kashatus

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miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro.

Roza K Przanowska, Ewelina Sobierajska, Zhangli Su, Kate Jensen, Piotr Przanowski, Sarbajeet Nagdas, Jennifer A Kashatus, David F Kashatus, Sanchita Bhatnagar, John R Lukens, Anindya Dutta,

miR-206, miR-1a-1, and miR-1a-2 are induced during differentiation of skeletal myoblasts and promote myogenesis in vitro. miR-206 is required for skeletal muscle regeneration in vivo. Although this miRNA family is hypothesized to play an essential role in differentiation, a triple knock-out (tKO) of the three genes has not been done ... Read more >>

FASEB J (FASEB journal : official publication of the Federation of American Societies for Experimental Biology)
[2020, 34(6):7687-7702]

Cited: 0 times

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PIM kinases alter mitochondrial dynamics and chemosensitivity in lung cancer.

Shailender S Chauhan, Rachel K Toth, Corbin C Jensen, Andrea L Casillas, David F Kashatus, Noel A Warfel,

Resistance to chemotherapy represents a major obstacle to the successful treatment of non-small-cell lung cancer (NSCLC). The goal of this study was to determine how PIM kinases impact mitochondrial dynamics, ROS production, and response to chemotherapy in lung cancer. Live-cell imaging and microscopy were used to determine the effect of ... Read more >>

Oncogene (Oncogene)
[2020, 39(12):2597-2611]

Cited: 1 time

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Drp1 Promotes KRas-Driven Metabolic Changes to Drive Pancreatic Tumor Growth.

Sarbajeet Nagdas, Jennifer A Kashatus, Aldo Nascimento, Syed S Hussain, Riley E Trainor, Sarah R Pollock, Sara J Adair, Alex D Michaels, Hiromi Sesaki, Edward B Stelow, Todd W Bauer, David F Kashatus,

Mitochondria undergo fission and fusion to maintain homeostasis, and tumors exhibit the dysregulation of mitochondrial dynamics. We recently demonstrated that ectopic HRasG12V promotes mitochondrial fragmentation and tumor growth through Erk phosphorylation of the mitochondrial fission GTPase Dynamin-related protein 1 (Drp1). However, the role of Drp1 in the setting of endogenous ... Read more >>

Cell Rep (Cell reports)
[2019, 28(7):1845-1859.e5]

Cited: 5 times

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RalA and RalB relocalization to depolarized mitochondria depends on clathrin-mediated endocytosis and facilitates TBK1 activation.

Sarah R Pollock, Austin R Schinlever, Ali Rohani, Jennifer A Kashatus, David F Kashatus,

Healthy mitochondria use an electrochemical gradient across the inner mitochondrial membrane (IMM) to generate energy in the form of ATP. A variety of endogenous and exogenous factors can lead to transient or sustained depolarization of the IMM, including mitochondrial fission events, expression of uncoupling proteins, electron transport chain (ETC) inhibitors, ... Read more >>

PLoS ONE (PloS one)
[2019, 14(4):e0214764]

Cited: 1 time

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MDVs: Spare the SOD and Spoil the Bug.

Syed Saad Hussain, David F Kashatus,

Mitochondrial reactive oxygen species are important anti-microbial weapons utilized by phagocytes of the innate immune system. In this issue of Cell Host and Microbe, Abuaita et al. (2018) show that in macrophages, mitochondrial-derived vesicles deliver the superoxide dismutase Sod2 to bacteria-containing phagosomes to produce hydrogen peroxide and kill invading bacteria. ... Read more >>

Cell Host Microbe (Cell host & microbe)
[2018, 24(5):616-618]

Cited: 0 times

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Mitochondrial protein S-nitrosation protects against ischemia reperfusion-induced denervation at neuromuscular junction in skeletal muscle.

Rebecca J Wilson, Joshua C Drake, Di Cui, Bevan M Lewellen, Carleigh C Fisher, Mei Zhang, David F Kashatus, Lisa A Palmer, Michael P Murphy, Zhen Yan,

Deterioration of neuromuscular junction (NMJ) integrity and function is causal to muscle atrophy and frailty, ultimately hindering quality of life and increasing the risk of death. In particular, NMJ is vulnerable to ischemia reperfusion (IR) injury when blood flow is restricted followed by restoration. However, little is known about the ... Read more >>

Free Radic. Biol. Med. (Free radical biology & medicine)
[2018, 117:180-190]

Cited: 5 times

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Conditional MitoTimer reporter mice for assessment of mitochondrial structure, oxidative stress, and mitophagy.

Rebecca J Wilson, Joshua C Drake, Di Cui, Mei Zhang, Heather M Perry, Jennifer A Kashatus, Christine M Kusminski, Philipp E Scherer, David F Kashatus, Mark D Okusa, Zhen Yan,

Assessment of structural and functional changes of mitochondria is vital for biomedical research as mitochondria are the power plants essential for biological processes and tissue/organ functions. Others and we have developed a novel reporter gene, pMitoTimer, which codes for a redox sensitive mitochondrial targeted protein that switches from green fluorescence ... Read more >>

Mitochondrion (Mitochondrion)
[2019, 44:20-26]

Cited: 6 times

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Dynamin-Related Protein 1 Deficiency Promotes Recovery from AKI.

Heather M Perry, Liping Huang, Rebecca J Wilson, Amandeep Bajwa, Hiromi Sesaki, Zhen Yan, Diane L Rosin, David F Kashatus, Mark D Okusa,

The proximal tubule epithelium relies on mitochondrial function for energy, rendering the kidney highly susceptible to ischemic AKI. Dynamin-related protein 1 (DRP1), a mediator of mitochondrial fission, regulates mitochondrial function; however, the cell-specific and temporal role of DRP1 in AKI in vivo is unknown. Using genetic murine models, we found ... Read more >>

J. Am. Soc. Nephrol. (Journal of the American Society of Nephrology : JASN)
[2018, 29(1):194-206]

Cited: 17 times

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The regulation of tumor cell physiology by mitochondrial dynamics.

David F Kashatus,

Mitochondrial dynamics are increasingly recognized to play an important role in regulating mitochondrial function in response to diverse stimuli. Given the overlap in the physiological processes influenced by mitochondria and the physiological processes disrupted in tumor cells, we speculate that tumor cells alter mitochondrial shape to promote the tumorigenic phenotype. ... Read more >>

Biochem Biophys Res Commun (Biochemical and biophysical research communications)
[2018, 500(1):9-16]

Cited: 4 times

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The Interplay between Oncogenic Signaling Networks and Mitochondrial Dynamics.

Sarbajeet Nagdas, David F Kashatus,

Mitochondria are dynamic organelles that alter their organization in response to a variety of cellular cues. Mitochondria are central in many biologic processes, such as cellular bioenergetics and apoptosis, and mitochondrial network morphology can contribute to those physiologic processes. Some of the biologic processes that are in part governed by ... Read more >>

Antioxidants (Basel) (Antioxidants (Basel, Switzerland))
[2017, 6(2):]

Cited: 6 times

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Restraining the Divider: A Drp1-Phospholipid Interaction Inhibits Drp1 Activity and Shifts the Balance from Mitochondrial Fission to Fusion.

David F Kashatus,

In this issue of Molecular Cell, Adachi et al. (2016) describe a novel interaction between the mitochondrial fission GTPase Drp1 and phosphatidic acid that restrains Drp1 activity and shifts the balance toward mitochondrial fusion, adding another layer of complexity to the regulation of mitochondrial dynamics. ... Read more >>

Mol. Cell (Molecular cell)
[2016, 63(6):913-915]

Cited: 5 times

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Erk2 phosphorylation of Drp1 promotes mitochondrial fission and MAPK-driven tumor growth.

Jennifer A Kashatus, Aldo Nascimento, Lindsey J Myers, Annie Sher, Frances L Byrne, Kyle L Hoehn, Christopher M Counter, David F Kashatus,

Ras is mutated in up to 30% of cancers, including 90% of pancreatic ductal adenocarcinomas, causing it to be constitutively GTP-bound, and leading to activation of downstream effectors that promote a tumorigenic phenotype. As targeting Ras directly is difficult, there is a significant effort to understand the downstream biological processes ... Read more >>

Mol. Cell (Molecular cell)
[2015, 57(3):537-551]

Cited: 163 times

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Ral GTPases in tumorigenesis: emerging from the shadows.

David F Kashatus,

Oncogenic Ras proteins rely on a series of key effector pathways to drive the physiological changes that lead to tumorigenic growth. Of these effector pathways, the RalGEF pathway, which activates the two Ras-related GTPases RalA and RalB, remains the most poorly understood. This review will focus on key developments in ... Read more >>

Exp Cell Res (Experimental cell research)
[2013, 319(15):2337-2342]

Cited: 25 times

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An in vitro system to evaluate the scaffold function of the RalA effector protein RalBP1.

David F Kashatus,

The in vitro kinase assay is an invaluable method to identify and characterize kinase substrates and to determine specific site(s) of phosphorylation. However, the use of recombinant proteins does not adequately account for the contribution of accessory proteins, such as scaffolds, that can contribute to the phosphorylation reaction in vivo. ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2014, 1120:207-216]

Cited: 0 times

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Targeting eNOS in pancreatic cancer.

Benjamin L Lampson, S Disean Kendall, Brooke B Ancrile, Meghan M Morrison, Michael J Shealy, Katharine S Barrientos, Matthew S Crowe, David F Kashatus, Rebekah R White, Susan B Gurley, Diana M Cardona, Christopher M Counter,

Mortality from pancreatic ductal adenocarcinoma cancer (PDAC) is among the highest of any cancer and frontline therapy has changed little in years. Activation of endothelial nitric oxide synthase (eNOS, NOS3, or NOS III) has been implicated recently in the pathogenesis of PDACs. In this study, we used genetically engineered mouse ... Read more >>

Cancer Res (Cancer research)
[2012, 72(17):4472-4482]

Cited: 26 times

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Breaking up is hard to do: RalA, mitochondrial fission and cancer.

David F Kashatus, Christopher M Counter,

The small GTPases RalA and RalB are activated downstream of oncogenic Ras. While activation of RalA is critically important for tumor initiation and growth of Ras-driven cancers, the highly similar small GTPase RalB is implicated in cell survival and metastasis. This difference in function between these two related proteins maps ... Read more >>

Small GTPases (Small GTPases)
[2011, 2(6):329-333]

Cited: 4 times

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RALA and RALBP1 regulate mitochondrial fission at mitosis.

David F Kashatus, Kian-Huat Lim, Donita C Brady, Nicole L K Pershing, Adrienne D Cox, Christopher M Counter,

Mitochondria exist as dynamic interconnected networks that are maintained through a balance of fusion and fission. Equal distribution of mitochondria to daughter cells during mitosis requires fission. Mitotic mitochondrial fission depends on both the relocalization of the large GTPase DRP1 to the outer mitochondrial membrane and phosphorylation of Ser 616 ... Read more >>

Nat Cell Biol (Nature cell biology)
[2011, 13(9):1108-1115]

Cited: 159 times

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cPLA2 regulates the expression of type I interferons and intracellular immunity to Chlamydia trachomatis.

Mark J Vignola, David F Kashatus, Gregory A Taylor, Christopher M Counter, Raphael H Valdivia,

Infection with the obligate bacterial intracellular pathogen Chlamydia trachomatis leads to the sustained activation of the small GTPase RAS and many of its downstream signaling components. In particular, the mitogen-activated protein kinase ERK and the calcium-dependent phospholipase cPLA(2) are activated and are important for the onset of inflammatory responses. In ... Read more >>

J. Biol. Chem. (The Journal of biological chemistry)
[2010, 285(28):21625-21635]

Cited: 24 times

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Aurora-A phosphorylates, activates, and relocalizes the small GTPase RalA.

Kian-Huat Lim, Donita C Brady, David F Kashatus, Brooke B Ancrile, Channing J Der, Adrienne D Cox, Christopher M Counter,

The small GTPase Ras, which transmits extracellular signals to the cell, and the kinase Aurora-A, which promotes proper mitosis, can both be inappropriately activated in human tumors. Here, we show that Aurora-A in conjunction with oncogenic Ras enhances transformed cell growth. Furthermore, such transformation and in some cases also tumorigenesis ... Read more >>

Mol. Cell. Biol. (Molecular and cellular biology)
[2010, 30(2):508-523]

Cited: 66 times

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Tumour maintenance is mediated by eNOS.

Kian-Huat Lim, Brooke B Ancrile, David F Kashatus, Christopher M Counter,

Tumour cells become addicted to the expression of initiating oncogenes like Ras, such that loss of oncogene expression in established tumours leads to tumour regression. HRas, NRas or KRas are mutated to remain in the active GTP-bound oncogenic state in many cancers. Although Ras activates several proteins to initiate human ... Read more >>

Nature (Nature)
[2008, 452(7187):646-649]

Cited: 157 times

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NF-kappa B and I kappa B alpha are found in the mitochondria. Evidence for regulation of mitochondrial gene expression by NF-kappa B.

Patricia C Cogswell, David F Kashatus, Jayne A Keifer, Denis C Guttridge, Julie Y Reuther, Cindy Bristow, Sophie Roy, Donald W Nicholson, Albert S Baldwin,

The transcription factor NF-kappa B has been shown to be predominantly cytoplasmically localized in the absence of an inductive signal. Stimulation of cells with inflammatory cytokines such as tumor necrosis factor alpha or interleukin-1 induces the degradation of I kappa B, the inhibitor of NF-kappa B, allowing nuclear accumulation of ... Read more >>

J. Biol. Chem. (The Journal of biological chemistry)
[2003, 278(5):2963-2968]

Cited: 101 times

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