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Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag.

Christine K Rourke, Darline Murat, Tyler J Hansen, Aimee Jaramillo-Lambert,

To investigate the dynamic localization of Topoisomerase II in live <i>C. elegans</i> we have generated a C-terminally GFP-tagged version of TOP-2 at the endogenous locus. We found that TOP-2::GFP localizes in a similar pattern to the previously published TOP-2::3XFLAG strain and does not disrupt the meiotic chromosome segregation functions of ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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