Full Text Journal Articles by
Author Buffy Stahl

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Improving End-User Trust in the Quality of Commercial Probiotic Products.

Scott A Jackson, Jean L Schoeni, Christina Vegge, Marco Pane, Buffy Stahl, Michael Bradley, Virginia S Goldman, Pierre Burguière, John B Atwater, Mary Ellen Sanders,

In a rapidly growing global probiotic market, end-users have difficulty distinguishing between high quality and poor quality products. This ambiguity threatens the trust consumers and healthcare providers have in probiotic products. To address this problem, we recommend that companies undergo third-party evaluations to certify probiotic quality and label accuracy. In ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2019, 10:739]

Cited: 20 times

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Nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge.

Markus J Lehtinen, Ashley A Hibberd, Sofia Männikkö, Nicolas Yeung, Tommi Kauko, Sofia Forssten, Liisa Lehtoranta, Sampo J Lahtinen, Buffy Stahl, Anna Lyra, Ronald B Turner,

The role of nasal and fecal microbiota in viral respiratory infections has not been established. We collected nasal swabs and washes, and fecal samples in a clinical study assessing the effect of probiotic Bifidobacterium animalis subsp. lactis Bl-04 on experimental rhinovirus infection. The nasal and fecal microbiota were characterized by ... Read more >>

Sci Rep (Scientific reports)
[2018, 8(1):11411]

Cited: 17 times

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Absolute Enumeration of Probiotic Strains Lactobacillus acidophilus NCFM® and Bifidobacterium animalis subsp. lactis Bl-04 ® via Chip-Based Digital PCR.

Sarah J Z Hansen, Wesley Morovic, Martha DeMeules, Buffy Stahl, Connie W Sindelar,

The current standard for enumeration of probiotics to obtain colony forming units by plate counts has several drawbacks: long time to results, high variability and the inability to discern between bacterial strains. Accurate probiotic cell counts are important to confirm the delivery of a clinically documented dose for its associated ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2018, 9:704]

Cited: 10 times

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Safety evaluation of HOWARU® Restore (Lactobacillus acidophilus NCFM, Lactobacillus paracasei Lpc-37, Bifidobacterium animalis subsp. lactis Bl-04 and B. lactis Bi-07) for antibiotic resistance, genomic risk factors, and acute toxicity.

Wesley Morovic, Jason M Roper, Amy B Smith, Pushkor Mukerji, Buffy Stahl, Jessica Caverly Rae, Arthur C Ouwehand,

Although probiotic lactobacilli and bifidobacteria are generally considered safe by various regulatory agencies, safety properties, such as absence of transferable antibiotic resistance, must still be determined for each strain prior to market introduction as a probiotic. Safety requirements for probiotics vary regionally and evaluation methods are not standardized, therefore methodologies ... Read more >>

Food Chem Toxicol (Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association)
[2017, 110:316-324]

Cited: 10 times

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Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases.

Wesley Morovic, Ashley A Hibberd, Bryan Zabel, Rodolphe Barrangou, Buffy Stahl,

Recent advances in microbiome research have brought renewed focus on beneficial bacteria, many of which are available in food and dietary supplements. Although probiotics have historically been defined as microorganisms that convey health benefits when ingested in sufficient viable amounts, this description now includes the stipulation "well defined strains," encompassing ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2016, 7:1747]

Cited: 16 times

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Safety evaluation of AB-LIFE(®) (Lactobacillus plantarum CECT 7527, 7528 and 7529): Antibiotic resistance and 90-day repeated-dose study in rats.

Pushkor Mukerji, Jason M Roper, Buffy Stahl, Amy B Smith, Frank Burns, Jessica Caverly Rae, Nicolas Yeung, Anna Lyra, Laura Svärd, Markku T Saarinen, Esa Alhoniemi, Alvin Ibarra, Arthur C Ouwehand,

AB-LIFE(®) is a probiotic product consisting of equal parts of three strains of Lactobacillus plantarum (CECT 7527, 7528, and 7529) blended with inert excipients. Whole genome sequencing was performed on each of the three strains. Antibiotic resistance was evaluated by genomic mining for resistance genes, and assessment for transferability. No ... Read more >>

Food Chem Toxicol (Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association)
[2016, 92:117-128]

Cited: 7 times

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Probiotic approach to prevent antibiotic resistance.

Arthur C Ouwehand, Sofia Forssten, Ashley A Hibberd, Anna Lyra, Buffy Stahl,

Probiotics are live microorganisms, mainly belonging to the genera Lactobacillus and Bifidobacterium, although also strain of other species are commercialized, that have a beneficial effect on the host. From the perspective of antibiotic use, probiotics have been observed to reduce the risk of certain infectious disease such as certain types ... Read more >>

Ann Med (Annals of medicine)
[2016, 48(4):246-255]

Cited: 33 times

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Genome-scale reconstruction of metabolic networks of Lactobacillus casei ATCC 334 and 12A.

Elena Vinay-Lara, Joshua J Hamilton, Buffy Stahl, Jeff R Broadbent, Jennifer L Reed, James L Steele,

Lactobacillus casei strains are widely used in industry and the utility of this organism in these industrial applications is strain dependent. Hence, tools capable of predicting strain specific phenotypes would have utility in the selection of strains for specific industrial processes. Genome-scale metabolic models can be utilized to better understand ... Read more >>

PLoS One (PloS one)
[2014, 9(11):e110785]

Cited: 14 times

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Genomic impact of CRISPR immunization against bacteriophages.

Rodolphe Barrangou, Anne-Claire Coûté-Monvoisin, Buffy Stahl, Isabelle Chavichvily, Florian Damange, Dennis A Romero, Patrick Boyaval, Christophe Fremaux, Philippe Horvath,

CRISPR (clustered regularly interspaced short palindromic repeats) together with CAS (RISPR-associated) genes form the CRISPR-Cas immune system, which provides sequence-specific adaptive immunity against foreign genetic elements in bacteria and archaea. Immunity is acquired by the integration of short stretches of invasive DNA as novel 'spacers' into CRISPR loci. Subsequently, these ... Read more >>

Biochem Soc Trans (Biochemical Society transactions)
[2013, 41(6):1383-1391]

Cited: 23 times

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Bifidobacterium animalis subsp. lactis ATCC 27673 is a genomically unique strain within its conserved subspecies.

Joseph R Loquasto, Rodolphe Barrangou, Edward G Dudley, Buffy Stahl, Chun Chen, Robert F Roberts,

Many strains of Bifidobacterium animalis subsp. lactis are considered health-promoting probiotic microorganisms and are commonly formulated into fermented dairy foods. Analyses of previously sequenced genomes of B. animalis subsp. lactis have revealed little genetic diversity, suggesting that it is a monomorphic subspecies. However, during a multilocus sequence typing survey of ... Read more >>

Appl Environ Microbiol (Applied and environmental microbiology)
[2013, 79(22):6903-6910]

Cited: 12 times

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Strong bias in the bacterial CRISPR elements that confer immunity to phage.

David Paez-Espino, Wesley Morovic, Christine L Sun, Brian C Thomas, Ken-ichi Ueda, Buffy Stahl, Rodolphe Barrangou, Jillian F Banfield,

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems provide adaptive immunity against phage via spacer-encoded CRISPR RNAs that are complementary to invasive nucleic acids. Here, we challenge Streptococcus thermophilus with a bacteriophage, and used PCR-based metagenomics to monitor phage-derived spacers daily for 15 days in two experiments. Spacers that target ... Read more >>

Nat Commun (Nature communications)
[2013, 4:1430]

Cited: 100 times

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Complete Genome Sequence of Probiotic Strain Lactobacillus acidophilus La-14.

Buffy Stahl, Rodolphe Barrangou,

We present the 1,991,830-bp complete genome sequence of Lactobacillus acidophilus strain La-14 (SD-5212). Comparative genomic analysis revealed 99.98% similarity overall to the L. acidophilus NCFM genome. Globally, 111 single nucleotide polymorphisms (SNPs) (95 SNPs, 16 indels) were observed throughout the genome. Also, a 416-bp deletion in the LA14_1146 sugar ABC ... Read more >>

Genome Announc (Genome announcements)
[2013, 1(3):]

Cited: 11 times

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Comparative whole-genome mapping to determine Staphylococcus aureus genome size, virulence motifs, and clonality.

Sanjay K Shukla, Madhulatha Pantrangi, Buffy Stahl, Adam M Briska, Mary E Stemper, Trevor K Wagner, Emily B Zentz, Steven M Callister, Steven D Lovrich, John K Henkhaus, Colin W Dykes,

Despite being a clonal pathogen, Staphylococcus aureus continues to acquire virulence and antibiotic-resistant genes located on mobile genetic elements such as genomic islands, prophages, pathogenicity islands, and the staphylococcal chromosomal cassette mec (SCCmec) by horizontal gene transfer from other staphylococci. The potential virulence of a S. aureus strain is often ... Read more >>

J Clin Microbiol (Journal of clinical microbiology)
[2012, 50(11):3526-3533]

Cited: 15 times

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Analysis of the Lactobacillus casei supragenome and its influence in species evolution and lifestyle adaptation.

Jeff R Broadbent, Eric C Neeno-Eckwall, Buffy Stahl, Kanokwan Tandee, Hui Cai, Wesley Morovic, Philippe Horvath, Jessie Heidenreich, Nicole T Perna, Rodolphe Barrangou, James L Steele,

<h4>Background</h4>The broad ecological distribution of L. casei makes it an insightful subject for research on genome evolution and lifestyle adaptation. To explore evolutionary mechanisms that determine genomic diversity of L. casei, we performed comparative analysis of 17 L. casei genomes representing strains collected from dairy, plant, and human sources.<h4>Results</h4>Differences in ... Read more >>

BMC Genomics (BMC genomics)
[2012, 13:533]

Cited: 71 times

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Complete genome sequences of probiotic strains Bifidobacterium animalis subsp. lactis B420 and Bi-07.

Buffy Stahl, Rodolphe Barrangou,

We present the complete genomes of Bifidobacterium animalis subsp. lactis B420 and Bi-07. Comparative genomic analysis with the type strain DSMZ10140 revealed 40 to 55 single nucleotide polymorphisms (SNPs) and an indel in a clustered regularly interspaced short palindromic repeat (CRISPR) locus. These genetic differences provide a molecular basis for ... Read more >>

J Bacteriol (Journal of bacteriology)
[2012, 194(15):4131-4132]

Cited: 11 times

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Use of optical mapping to sort uropathogenic Escherichia coli strains into distinct subgroups.

William R Schwan, Adam Briska, Buffy Stahl, Trevor K Wagner, Emily Zentz, John Henkhaus, Steven D Lovrich, William A Agger, Steven M Callister, Brian DuChateau, Colin W Dykes,

Optical maps were generated for 33 uropathogenic Escherichia coli (UPEC) isolates. For individual genomes, the NcoI restriction fragments aligned into a unique chromosome map for each individual isolate, which was then compared with the in silico restriction maps of all of the sequenced E. coli and Shigella strains. All of ... Read more >>

Microbiology (Reading) (Microbiology (Reading, England))
[2010, 156(Pt 7):2124-2135]

Cited: 18 times

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Emergent properties of reduced-genome Escherichia coli.

György Pósfai, Guy Plunkett, Tamás Fehér, David Frisch, Günther M Keil, Kinga Umenhoffer, Vitaliy Kolisnychenko, Buffy Stahl, Shamik S Sharma, Monika de Arruda, Valerie Burland, Sarah W Harcum, Frederick R Blattner,

With the use of synthetic biology, we reduced the Escherichia coli K-12 genome by making planned, precise deletions. The multiple-deletion series (MDS) strains, with genome reductions up to 15%, were designed by identifying nonessential genes and sequences for elimination, including recombinogenic or mobile DNA and cryptic virulence genes, while preserving ... Read more >>

Science (Science (New York, N.Y.))
[2006, 312(5776):1044-1046]

Cited: 340 times

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