Full Text Journal Articles by
Author Ashleigh B Theberge

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Localized Cell-Surface Sampling of a Secreted Factor Using Cell-Targeting Beads.

Tammi L van Neel, Samuel B Berry, Erwin Berthier, Ashleigh B Theberge,

Intercellular communication through the secretion of soluble factors plays a vital role in a wide range of biological processes (e.g., homeostasis, immune response), yet identification and quantification of many of these factors can be challenging due to their degradation or sequestration in cell culture media prior to analysis. Here, we ... Read more >>

Anal Chem (Analytical chemistry)
[2020, 92(20):13634-13640]

Cited: 0 times

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Open-Channel Capillary Trees and Capillary Pumping.

Jing J Lee, Jean Berthier, Kathleen E Kearney, Erwin Berthier, Ashleigh B Theberge,

Velocity of capillary flow in closed or open channels decreases as the flow proceeds down the length of the channel, varying as the inverse of the square root of time or as the inverse of travel distance. In order to increase the flow rate-and extend the duration of the flow-capillary ... Read more >>

Langmuir (Langmuir : the ACS journal of surfaces and colloids)
[2020, :]

Cited: 0 times

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Host and Pathogen Communication in the Respiratory Tract: Mechanisms and Models of a Complex Signaling Microenvironment.

Samuel B Berry, Amanda J Haack, Ashleigh B Theberge, Susanna Brighenti, Mattias Svensson,

Chronic lung diseases are a leading cause of morbidity and mortality across the globe, encompassing a diverse range of conditions from infections with pathogenic microorganisms to underlying genetic disorders. The respiratory tract represents an active interface with the external environment having the primary immune function of resisting pathogen intrusion and ... Read more >>

Front Med (Lausanne) (Frontiers in medicine)
[2020, 7:537]

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Open microfluidic coculture reveals paracrine signaling from human kidney epithelial cells promotes kidney specificity of endothelial cells.

Tianzi Zhang, Daniel Lih, Ryan J Nagao, Jun Xue, Erwin Berthier, Jonathan Himmelfarb, Ying Zheng, Ashleigh B Theberge,

Endothelial cells (ECs) from different human organs possess organ-specific characteristics that support specific tissue regeneration and organ development. EC specificity is identified by both intrinsic and extrinsic cues, among which the parenchyma and organ-specific microenvironment are critical contributors. These extrinsic cues are, however, largely lost during ex vivo cultures. Outstanding ... Read more >>

Am J Physiol Renal Physiol (American journal of physiology. Renal physiology)
[2020, 319(1):F41-F51]

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Layer-by-layer fabrication of 3D hydrogel structures using open microfluidics.

Ulri N Lee, John H Day, Amanda J Haack, Ross C Bretherton, Wenbo Lu, Cole A DeForest, Ashleigh B Theberge, Erwin Berthier,

Patterned deposition and 3D fabrication techniques have enabled the use of hydrogels for a number of applications including microfluidics, sensors, separations, and tissue engineering in which form fits function. Devices such as reconfigurable microvalves or implantable tissues have been created using lithography or casting techniques. Here, we present a novel ... Read more >>

Lab Chip (Lab on a chip)
[2020, 20(3):525-536]

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Injection molded open microfluidic well plate inserts for user-friendly coculture and microscopy.

John H Day, Tristan M Nicholson, Xiaojing Su, Tammi L van Neel, Ivor Clinton, Anbarasi Kothandapani, Jinwoo Lee, Max H Greenberg, John K Amory, Thomas J Walsh, Charles H Muller, Omar E Franco, Colin R Jefcoate, Susan E Crawford, Joan S Jorgensen, Ashleigh B Theberge,

Open microfluidic cell culture systems are powerful tools for interrogating biological mechanisms. We have previously presented a microscale cell culture system, based on spontaneous capillary flow of biocompatible hydrogels, that is integrated into a standard cell culture well plate, with flexible cell compartment geometries and easy pipet access. Here, we ... Read more >>

Lab Chip (Lab on a chip)
[2020, 20(1):107-119]

Cited: 0 times

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Droplet Incubation and Splitting in Open Microfluidic Channels.

Samuel B Berry, Jing J Lee, Jean Berthier, Erwin Berthier, Ashleigh B Theberge,

Droplet-based microfluidics enables compartmentalization and controlled manipulation of small volumes. Open microfluidics provides increased accessibility, adaptability, and ease of manufacturing compared to closed microfluidic platforms. Here, we begin to build a toolbox for the emerging field of open channel droplet-based microfluidics, combining the ease of use associated with open microfluidic ... Read more >>

Anal Methods (Analytical methods : advancing methods and applications)
[2019, 11(35):4528-4536]

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3D printed coaxial nozzles for the extrusion of hydrogel tubes toward modeling vascular endothelium.

S Cem Millik, Ashley M Dostie, Dylan G Karis, Patrick T Smith, Michael McKenna, Nathan Chan, Chad D Curtis, Elizabeth Nance, Ashleigh B Theberge, Alshakim Nelson,

Engineered tubular constructs made from soft biomaterials are employed in a myriad of applications in biomedical science. Potential uses of these constructs range from vascular grafts to conduits for enabling perfusion of engineered tissues and organs. The fabrication of standalone tubes or complex perfusable constructs from biofunctional materials, including hydrogels, ... Read more >>

Biofabrication (Biofabrication)
[2019, 11(4):045009]

Cited: 1 time

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Investigating Fibroblast-Induced Collagen Gel Contraction Using a Dynamic Microscale Platform.

Tianzi Zhang, John H Day, Xiaojing Su, Arthur G Guadarrama, Nathan K Sandbo, Stephane Esnault, Loren C Denlinger, Erwin Berthier, Ashleigh B Theberge,

Mechanical forces have long been recognized as fundamental drivers in biological processes, such as embryogenesis, tissue formation and disease regulation. The collagen gel contraction (CGC) assay has served as a classic tool in the field of mechanobiology to study cell-induced contraction of extracellular matrix (ECM), which plays an important role ... Read more >>

(Frontiers in bioengineering and biotechnology)
[2019, 7:196]

Cited: 1 time

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Capillary Flow in Open Microgrooves: Bifurcations and Networks.

Jing J Lee, Jean Berthier, Ashleigh B Theberge, Erwin Berthier,

Open capillary flows are increasingly used in biotechnology, biology, thermics, and space science. So far, the dynamics of capillary flows has been studied mostly for confined channels. However, the theory of open microfluidics has considerably progressed during the last years, and an expression for the travel distance has been derived, ... Read more >>

Langmuir (Langmuir : the ACS journal of surfaces and colloids)
[2019, 35(32):10667-10675]

Cited: 0 times

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Stable biphasic interfaces for open microfluidic platforms.

Ulri N Lee, Jean Berthier, Jiaquan Yu, Erwin Berthier, Ashleigh B Theberge,

We present an open microfluidic platform that enables stable flow of an organic solvent over an aqueous solution. The device features apertures connecting a lower aqueous channel to an upper solvent compartment that is open to air, enabling easy removal of the solvent for analysis. We have previously shown that ... Read more >>

Biomed Microdevices (Biomedical microdevices)
[2019, 21(1):16]

Cited: 2 times

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Discovery of glycocholic acid and taurochenodeoxycholic acid as phenotypic biomarkers in cholangiocarcinoma.

Won-Suk Song, Hae-Min Park, Jung Min Ha, Sung Gyu Shin, Han-Gyu Park, Joonwon Kim, Tianzi Zhang, Da-Hee Ahn, Sung-Min Kim, Yung-Hun Yang, Jae Hyun Jeong, Ashleigh B Theberge, Byung-Gee Kim, Jong Kyun Lee, Yun-Gon Kim,

Although several biomarkers can be used to distinguish cholangiocarcinoma (CCA) from healthy controls, differentiating the disease from benign biliary disease (BBD) or pancreatic cancer (PC) is a challenge. CCA biomarkers are associated with low specificity or have not been validated in relation to the biological effects of CCA. In this ... Read more >>

Sci Rep (Scientific reports)
[2018, 8(1):11088]

Cited: 5 times

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Droplet Behavior in Open Biphasic Microfluidics.

Jing J Lee, Jean Berthier, Kenneth A Brakke, Ashley M Dostie, Ashleigh B Theberge, Erwin Berthier,

Capillary open microsystems are attractive and increasingly used in biotechnology, biology, and diagnostics as they allow simple and reliable control of fluid flows. In contrast to closed microfluidic systems, however, two-phase capillary flows in open microfluidics have remained largely unexplored. In this work, we present the theoretical basis and experimental ... Read more >>

Langmuir (Langmuir : the ACS journal of surfaces and colloids)
[2018, 34(18):5358-5366]

Cited: 1 time

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Fundamentals of rapid injection molding for microfluidic cell-based assays.

Ulri N Lee, Xiaojing Su, David J Guckenberger, Ashley M Dostie, Tianzi Zhang, Erwin Berthier, Ashleigh B Theberge,

Microscale cell-based assays have demonstrated unique capabilities in reproducing important cellular behaviors for diagnostics and basic biological research. As these assays move beyond the prototyping stage and into biological and clinical research environments, there is a need to produce microscale culture platforms more rapidly, cost-effectively, and reproducibly. 'Rapid' injection molding ... Read more >>

Lab Chip (Lab on a chip)
[2018, 18(3):496-504]

Cited: 2 times

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Upgrading well plates using open microfluidic patterning.

Samuel B Berry, Tianzi Zhang, John H Day, Xiaojing Su, Ilham Z Wilson, Erwin Berthier, Ashleigh B Theberge,

Cellular communication between multiple cell types is a ubiquitous process that is responsible for vital physiological responses observed in vivo (e.g., immune response, organ function). Many in vitro coculture strategies have been developed, both in traditional culture and microscale systems, and have shown the potential to recreate some of the ... Read more >>

Lab Chip (Lab on a chip)
[2017, 17(24):4253-4264]

Cited: 5 times

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Microbial volatile communication in human organotypic lung models.

Layla J Barkal, Clare L Procknow, Yasmín R Álvarez-García, Mengyao Niu, José A Jiménez-Torres, Rebecca A Brockman-Schneider, James E Gern, Loren C Denlinger, Ashleigh B Theberge, Nancy P Keller, Erwin Berthier, David J Beebe,

We inhale respiratory pathogens continuously, and the subsequent signaling events between host and microbe are complex, ultimately resulting in clearance of the microbe, stable colonization of the host, or active disease. Traditional in vitro methods are ill-equipped to study these critical events in the context of the lung microenvironment. Here ... Read more >>

Nat Commun (Nature communications)
[2017, 8(1):1770]

Cited: 8 times

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Multikingdom microscale models.

Layla J Barkal, Erwin Berthier, Ashleigh B Theberge, Nancy P Keller, David J Beebe,

PLoS Pathog. (PLoS pathogens)
[2017, 13(8):e1006424]

Cited: 1 time

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Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis.

Ashleigh B Theberge, Jiaquan Yu, Edmond W K Young, William A Ricke, Wade Bushman, David J Beebe,

Angiogenesis (the formation of blood vessels from existing blood vessels) plays a critical role in many diseases such as cancer, benign tumors, and macular degeneration. There is a need for cell culture methods capable of dissecting the intricate regulation of angiogenesis within the microenvironment of the vasculature. We have developed ... Read more >>

Anal Chem (Analytical chemistry)
[2015, 87(6):3239-3246]

Cited: 15 times

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Cellular microenvironment dictates androgen production by murine fetal Leydig cells in primary culture.

Colleen M Carney, Jessica L Muszynski, Lindsay N Strotman, Samantha R Lewis, Rachel L O'Connell, David J Beebe, Ashleigh B Theberge, Joan S Jorgensen,

Despite the fact that fetal Leydig cells are recognized as the primary source of androgens in male embryos, the mechanisms by which steroidogenesis occurs within the developing testis remain unclear. A genetic approach was used to visualize and isolate fetal Leydig cells from remaining cells within developing mouse testes. Cyp11a1-Cre ... Read more >>

Biol. Reprod. (Biology of reproduction)
[2014, 91(4):85]

Cited: 6 times

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Suspended microfluidics.

Benjamin P Casavant, Erwin Berthier, Ashleigh B Theberge, Jean Berthier, Sara I Montanez-Sauri, Lauren L Bischel, Kenneth Brakke, Curtis J Hedman, Wade Bushman, Nancy P Keller, David J Beebe,

Although the field of microfluidics has made significant progress in bringing new tools to address biological questions, the accessibility and adoption of microfluidics within the life sciences are still limited. Open microfluidic systems have the potential to lower the barriers to adoption, but the absence of robust design rules has ... Read more >>

Proc. Natl. Acad. Sci. U.S.A. (Proceedings of the National Academy of Sciences of the United States of America)
[2013, 110(25):10111-10116]

Cited: 41 times

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Renal capsule xenografting and subcutaneous pellet implantation for the evaluation of prostate carcinogenesis and benign prostatic hyperplasia.

Tristan M Nicholson, Kristen S Uchtmann, Conrad D Valdez, Ashleigh B Theberge, Tihomir Miralem, William A Ricke,

New therapies for two common prostate diseases, prostate cancer (PrCa) and benign prostatic hyperplasia (BPH), depend critically on experiments evaluating their hormonal regulation. Sex steroid hormones (notably androgens and estrogens) are important in PrCa and BPH; we probe their respective roles in inducing prostate growth and carcinogenesis in mice with ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2013, (78):]

Cited: 4 times

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Effect of microculture on cell metabolism and biochemistry: do cells get stressed in microchannels?

Xiaojing Su, Ashleigh B Theberge, Craig T January, David J Beebe,

Microfluidics is emerging as a promising platform for cell culture, enabling increased microenvironment control and potential for integrated analysis compared to conventional macroculture systems such as well plates and Petri dishes. To advance the use of microfluidic devices for cell culture, it is necessary to better understand how miniaturization affects ... Read more >>

Anal Chem (Analytical chemistry)
[2013, 85(3):1562-1570]

Cited: 12 times

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Investigation of "on water" conditions using a biphasic fluidic platform.

Sonia Mellouli, Lise Bousekkine, Ashleigh B Theberge, Wilhelm T S Huck,

Angew. Chem. Int. Ed. Engl. (Angewandte Chemie (International ed. in English))
[2012, 51(32):7981-7984]

Cited: 7 times

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Microfluidic platform for combinatorial synthesis in picolitre droplets.

Ashleigh B Theberge, Estelle Mayot, Abdeslam El Harrak, Felix Kleinschmidt, Wilhelm T S Huck, Andrew D Griffiths,

This paper presents a droplet-based microfluidic platform for miniaturized combinatorial synthesis. As a proof of concept, a library of small molecules for early stage drug screening was produced. We present an efficient strategy for producing a 7 × 3 library of potential thrombin inhibitors that can be utilized for other ... Read more >>

Lab Chip (Lab on a chip)
[2012, 12(7):1320-1326]

Cited: 34 times

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Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology.

Ashleigh B Theberge, Fabienne Courtois, Yolanda Schaerli, Martin Fischlechner, Chris Abell, Florian Hollfelder, Wilhelm T S Huck,

Microdroplets in microfluidics offer a great number of opportunities in chemical and biological research. They provide a compartment in which species or reactions can be isolated, they are monodisperse and therefore suitable for quantitative studies, they offer the possibility to work with extremely small volumes, single cells, or single molecules, ... Read more >>

Angew. Chem. Int. Ed. Engl. (Angewandte Chemie (International ed. in English))
[2010, 49(34):5846-5868]

Cited: 229 times

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