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Author Aseem Tiwari

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Development of a Novel Multiplex Bead-based Assay for Measuring Autoantibodies on Flow Cytometric Platform.

Rajni Chauhan, Nikita Gupta, Aseem Kumar Tiwari, Vimarsh Raina, Shoma Paul Nandi,

<b>Background</b>: Autoantibodies (AAbs) are important biomarkers for the diagnosis of Autoimmune Diseases (ADs). The detection of AAbs performed by current methods (indirect immunofluorescence test (IIFT)/Immunoblot (dot/line)/enzyme-linked immunosorbent assay ELISA) which have limitations in terms of performing multiple assays to arrive at laboratory diagnosis. We validated a novel multiplex bead-based assay ... Read more >>

Immunol Invest (Immunological investigations)
[2020, :1-14]

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An algorithmic approach to serological work-up of ABO sub-groups which present as ABO discrepancies in resource constraint settings.

Aseem K Tiwari, Divya Setya, Dinesh Arora, Swati Pabbi Mehta, Geet Aggarwal, Subhasis Mitra,

<h4>Background</h4>ABO subgroups or weaker variants of A or B are group A or B subjects whose erythrocytes give a weak or negative reaction serologically with anti-A or Anti - B antisera respectively. Occurrence of these subgroups may lead to an ABO discrepancy which often puts transfusion services in a quandary. ... Read more >>

J Immunol Methods (Journal of immunological methods)
[2020, 487:112895]

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Laboratory Diagnosis of ANCA-Associated Vasculitis (AAV) Using a Combination of Immunofluorescence Test (IIFT) and Line Immunoassay (LIA): Single-Centre Report From India.

Rajni Chauhan, Dharmendra Jain, Aseem Kumar Tiwari, Pranav Dorwal, Vimarsh Raina, Shoma Paul Nandi,

<h4>Introduction</h4>Anti-neutrophil cytoplasmic autoantibodies (ANCA) associated vasculitis (AAV) is a small vessel vasculitis with insufficient epidemiological estimates in India. We aimed to determine demographic, clinical features, and laboratory diagnosis of AAV patients presenting to a large tertiary care centre in India.<h4>Material and methods</h4>1289 patient samples were screened for ANCA by indirect ... Read more >>

Reumatol Clin (Reumatologia clinica)
[2020, :]

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Routine indirect antiglobulin testing of blood donors-a further step toward blood safety: an experience from a tertiary care center in northern India.

Sheetal Malhotra, Gita Negi, Daljit Kaur, Sushant K Meinia, Aseem K Tiwari, Subhasis Mitra,

<h4>Conclusions</h4>This scientific article emphasizes the importance of a policy for antibody screening of all blood donors as a step to further improve blood safety. We also report the incidence of red blood cell (RBC) alloimmunization in healthy blood donors obtained using a cross-sectional prospective study from September 2017 to January ... Read more >>

Immunohematology (Immunohematology)
[2020, 36(3):93-98]

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Severe hemolytic disease of the fetus and newborn due to anti-E and anti-Jka.

Saikat Mandal, Sheetal Malhotra, Gita Negi, Aseem Tiwari, Subhasis Mitra, Sriparna Basu, Poonam Singh,

<h4>Conclusions</h4>Red blood cell alloimmunization to antigens other than D, such as C, c, E, e, and antigens in the Kell, MNS, and Duffy blood group systems, has emerged as an important cause of hemolytic disease of the fetus and newborn (HDFN). Antibody screening for these antibodies is not routinely practiced ... Read more >>

Immunohematology (Immunohematology)
[2020, 36(2):60-63]

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The frequent and the unusual red cell phenotypes in Indian blood donors: A quest for rare donors.

Divya Setya, Aseem Kumar Tiwari, Dinesh Arora, Subhasis Mitra, Swati Pabbi Mehta, Geet Aggarwal,

A clinically significant red cell alloantibody is capable of accelerated destruction of red cells bearing the corresponding antigen. Knowledge of prevalence of these antigens is necessary for performing day to day work and for research in immunohematology. The primary aim of this study was to find the prevalence of 18 ... Read more >>

Transfus Apher Sci (Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis)
[2020, 59(4):102765]

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Utility of grey zone testing strategy in transfusion transmissible infection testing in blood bank is of limited value!

Gunjan Bhardwaj, Aseem K Tiwari, Dinesh Arora, Geet Aggarwal, Swati Pabbi, Divya Setya,

Several blood banks use grey zone (GZ) phenomenon (defined as samples with optical density within 10% below the cut off in enzyme immuno-assay [EIA]/chemiluminescence immunoassay [CLIA]) to further augment blood safety. There is paucity of data regarding usefulness of GZ sample and its application in Transfusion Transmissible Infection (TTI) screening ... Read more >>

Indian J Pathol Microbiol (Indian journal of pathology & microbiology)
[2020, 63(2):255-257]

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Patient blood management in a patient with multiple red cell antibodies (anti-C, anti-e, and anti-K) undergoing liver transplant in South India: A team approach.

Deepti Sachan, Aseem K Tiwari, Ravi Dara, Dinesh Jothimani, Ilankumaran Kaliamoorthy, Srinivas Mettu Reddy, Mohamed Rela,

End-stage liver disease (ESLD) patients undergoing liver transplant (LT) surgery are often multiply alloimmunized and pose significant challenges to the transfusion services in terms of red cell cross-match incompatibility, unpredictable blood requirements, and often lead to significant delays in availing compatible red cell units. We report a case of a ... Read more >>

Asian J Transfus Sci (Asian journal of transfusion science)
[2020, 14(1):74-78]

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Applying Donath-Landsteiner test for the diagnosis of paroxysmal cold hemoglobinuria.

Aseem Kumar Tiwari, Geet Aggarwal, Subhasis Mitra, Dinesh Arora, Gunjan Bhardwaj, Ankita Ratan, Divya Setya,

61-year old male patient was admitted to the hospital with clinical picture of hemolytic anemia with hemoglobinuria. Patient was suspected to have Infectious Mononucleosis (IM) with Auto Immune Hemolytic Anemia (AIHA). DAT was positive with anti-C3d specificity. Donath Landsteiner (DL) test was positive; confirming Paroxysmal Cold Hemoglobinuria (PCH). The final ... Read more >>

Asian J Transfus Sci (Asian journal of transfusion science)
[2020, 14(1):57-59]

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Immunoadsorption in ABO-incompatible kidney transplantation in adult and pediatric patients with follow-up on graft and patient survival: First series from India.

Aseem Kumar Tiwari, Geet Aggarwal, Dinesh Arora, Gunjan Bhardwaj, Manish Jain, Shyam Bihari Bansal, Sidharth K Sethi,

<h4>Background</h4>There are no published reports on desensitization protocol for ABO-incompatible kidney transplants using Immuno-Adsorption (IA) plasmapheresis from India. IA offers certain advantages including processing of larger plasma volumes, quicker reduction of isoagglutinin titers and no requirement of replacement fluids.<h4>Aims and objectives</h4>Authors' center evaluated success of desensitization protocol, and graft/patient outcomes ... Read more >>

Asian J Transfus Sci (Asian journal of transfusion science)
[2020, 14(1):13-18]

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Red blood cell exchange in sickle cell disease patient with multiple alloantibodies.

Geet Aggarwal, Aseem Kumar Tiwari, Pratibha Dhiman, Dinesh Arora, Swati Mehta Pabbi, Divya Setya,

There are several reports in medical literature about Red Cell Exchange (RCE) being routinely performed pre-operatively in sickle cell disease patients to provide immediate decrease in HbS concentration and prevent post-operative complications. We would like to present one such case of SCD who also had multiple allo-antibodies and had to ... Read more >>

Asian J Transfus Sci (Asian journal of transfusion science)
[2020, 14(1):70-73]

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Prophylactic administration of oral calcium carbonate during plateletpheresis: A bicentric prospective study.

Prashant Kumar Pandey, Aseem Tiwari, Nitin Agarwal, Ravi C Dara,

<h4>Background</h4>Administration of anticoagulant citrate and dextrose (ACD-A) chelates ionized calcium in blood and causes hypocalcemia in plateletpheresis donors. The aim of the study was to observe the effects of oral calcium (Ca) supplementation during plateletpheresis on various parameters related to calcium metabolism.<h4>Materials and methods</h4>This study was performed between January 2014 ... Read more >>

Asian J Transfus Sci (Asian journal of transfusion science)
[2020, 14(1):19-22]

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Pre-transplant Compatibility Tests in Kidney Transplants: Case Report on Significance of Epitope-based Analysis in Donor Selection.

Chhavi Rajvanshi, Aseem Kumar Tiwari, Jui Choudhuri, Simmi Mehra, Rajni Chauhan,

Indian J Nephrol (Indian journal of nephrology)
[2020, 30(1):57-58]

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Discordance between Flow-Cytometry Crossmatch and Single Antigen Bead (SAB) Assay: An Uncommon Finding and its Resolution.

Simmi Mehra, Aseem Kumar Tiwari, Geet Aggarwal, Dinesh Arora, Abhishek Saini, Chhavi Rajvanshi, Poonam Kumari, Pranav Dorwal,

Indian J Nephrol (Indian journal of nephrology)
[2020, 30(1):55-57]

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Application of sequential serological testing strategy for detection of Hepatitis B surface antigen (HBsAg) for diagnosing HBV infection.

Aseem Kumar Tiwari, Swati Pabbi, Geet Aggarwal, Dinesh Arora, Gunjan Bhardwaj, Divya Setya, Jyoti Sharma,

The diagnostic accuracy of any serological test for detection of HBsAg is not 100%. We hypothesized that the sequential testing strategy proven for anti-HIV laboratory diagnosis should also apply to other infectious disease markers like HBsAg. Therefore, we evaluated the diagnostic accuracy of these strategies, I (single test), II (two ... Read more >>

J Virol Methods (Journal of virological methods)
[2019, 274:113726]

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Comparison of a column agglutination technology-based automated immunohematology analyzer and a semiautomated system in pretransfusion testing.

Ravi C Dara, Aseem Kumar Tiwari, Subhasis Mitra, Deviprasad Acharya, Geet Aggarwal, Dinesh Arora, Gunjan Bhardwaj,

INTRODUCTION:Semi-automated equipment using Column agglutination technology (CAT) is widely used where centrifugation and incubation are automated but substantial amount of the work is still executed manually. Larger laboratories are moving towards automation to eliminate errors, reducing exposure to bio-hazardous samples, assuring traceability, reliability, turnaround time (TAT) and throughput. Moving towards ... Read more >>

Asian J Transfus Sci (Asian journal of transfusion science)
[2019, 13(2):115-119]

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Matched unrelated donor hematopoietic progenitor cell transplantation: A report based on a single registry in India.

Aseem K Tiwari, Vikash Chandra Mishra, Apeksha Tiwari, Pranav Dorwal, Kapil Gupta, Dinesh Chandra, Vimarsh Raina,

<h4>Introduction</h4>Currently, more than 10,000 matched unrelated donor transplants (MUDT) are performed annually worldwide. India has recorded a significant increase in the number of hematopoietic progenitor cell transplantation (HPCT) centers reporting transplants. The number of HPCTs increases by approximately 10% every year, with 1878 transplants reported by Indian stem cell transplant ... Read more >>

Leuk Res Rep (Leukemia research reports)
[2019, 11:17-20]

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A guide to organize voluntary stem cell donors recruitment drive for hematopoietic progenitor stem cell transplant.

Vikash Chandra Mishra, Aseem K Tiwari, Pranav Dorwal, Vimarsh Raina, Girish Sharma,

<h4>Background</h4>Hematopoietic progenitor cell transplantation (HPCT) is used as a definitive treatment in hematological malignancies. For a successful HPCT, the donor and recipient should have matching human leukocyte antigens (HLAs). About 25% of patients have a chance of finding matching HLA within family, while rests 75% are dependent on voluntary stem ... Read more >>

Asian J Transfus Sci (Asian journal of transfusion science)
[2019, 13(1):39-42]

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Successful treatment of Sezary syndrome with extracorporeal photopheresis - The first attempt in India.

Aseem K Tiwari, Dinesh Arora, Pratibha Dhiman, Sheilly Kapoor, Geet Aggarwal, Ravi C Dara, Ashok Vaid,

Sezary syndrome (SS) is more aggressive leukemic variant of cutaneous T-cell lymphoma in which a significant number of circulating malignant (Sezary) cells are observed in peripheral blood. Although single-agent or combination chemotherapy regimens have produced moderately high response rates in patients with advanced-stage SS, these responses are invariably not durable. ... Read more >>

Asian J Transfus Sci (Asian journal of transfusion science)
[2019, 13(1):66-69]

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Applying newer parameter Ret-He (reticulocyte haemoglobin equivalent) to assess latent iron deficiency (LID) in blood donors-study at a tertiary care hospital in India.

Aseem K Tiwari, Gunjan Bhardwaj, Dinesh Arora, Geet Aggarwal, Swati Pabbi, Ravi C Dara, Ritesh Sachdev, Arun Raizada, Monisha Sethi,

<h4>Background</h4>It is important to detect Latent Iron Deficiency (LID) to prevent development of an overt iron deficiency anemia. Early detection is difficult by using conventional hematological and biochemical parameters. Soluble transferrin receptor (sTfR) is presently the gold standard for diagnosing LID. We evaluated the utility of Reticulocyte Hemoglobin Equivalent (Ret-He), ... Read more >>

Vox Sang (Vox sanguinis)
[2018, 113(7):639-646]

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Severe hypertriglyceridemia-induced pancreatitis successfully managed with therapeutic plasma exchange: Report from India.

Swati Pabbi Mehta, Aseem Kumar Tiwari, Rajesh Puri, Dinesh Arora, Geet Aggarwal, Ankita Ratan,

Hypertriglyceridemia (HTG) is the third most significant risk factor for acute pancreatitis after gallstones and alcohol. Therapeutic plasma exchange (TPE) has been considered a possible treatment for HTG-induced pancreatitis, especially in severe and refractory cases. Here, we report one such clinical experience with a patient of severe HTG-induced pancreatitis. He ... Read more >>

Asian J Transfus Sci (Asian journal of transfusion science)
[2018, 12(2):154-156]

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Clinically significant naturally occurring anti-N and anti-S in a blood donor: a rare finding.

Sheetal Malhotra, Gita Negi, Aseem K Tiwari,

<h4>Conclusions</h4>To the Editors: Alloimmunization is triggered when an individual whose red blood cells (RBCs) are lacking particular antigens is exposed to these antigens through transfusion or pregnancy, causing the formation of immune antibodies. In addition to these exogenous exposures, underlying inflammatory or autoimmune conditions may lead to formation of unexpected ... Read more >>

Immunohematology (Immunohematology)
[2018, 34(2):66-68]

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Notification and counselling of hepatitis positive blood donors, their immediate emotional response, contact-testing and their follow-up: Study from a tertiary care hospital!

Aseem K Tiwari, Gunjan Bhardwaj, Ravi C Dara, Dinesh Arora, Geet Aggarwal, Richa Bhargava, Kaushal Madan,

INTRODUCTION:Post-donation counselling informs donors of unusual test results. Timely notification and counselling regarding their Transfusion Transmitted Infection (TTI) status is necessary for early clinical intervention in the donor and reducing risk of transmission. We share our experience with respect to Hepatitis B (HBV) and Hepatitis C (HCV) positive donors who ... Read more >>

Transfus Apher Sci (Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis)
[2018, 57(3):391-397]

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Pediatric ABO-incompatible kidney transplantation: Evolving with the advancing apheresis technology: A single-center experience.

Sidharth Kumar Sethi, Shyam Bihari Bansal, Nikita Wadhwani, Aseem Tiwari, Dinesh Arora, Reetesh Sharma, Ashish Nandwani, Dinesh Kumar Yadav, Amit Kumar Mahapatra, Manish Jain, Pranaw Jha, Prasun Ghosh, Anil Bhan, Maninder Dhaliwal, Veena Raghunathan, Vijay Kher,

Recent literature has endorsed favorable outcomes following ABOi kidney transplantation in pediatric population. Nevertheless, reluctance to pursue an ABOi still remains pervasive. This could be ascribed to various legitimate reasons, namely less extensive pediatric ABOi data, technical difficulties encountered during PP, cost restraints, and concerns regarding higher rates of antibody-mediated ... Read more >>

Pediatr Transplant (Pediatric transplantation)
[2018, 22(3):e13138]

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Advantages of type and screen policy: Perspective from a developing country!

Geet Aggarwal, Aseem K Tiwari, Dinesh Arora, Ravi C Dara, Devi P Acharya, Gunjan Bhardwaj, Jyoti Sharma,

INTRODUCTION:The authors' center recently changed their pretransfusion testing protocol from "conventional" type and screen (TS) with anti-human globulin (AHG) crossmatch (Policy A) to TS with immediate-spin (IS) crossmatch (Policy B). Red blood cell (RBC) units were issued after compatible IS crossmatch as and when required instead of AHG crossmatch. This ... Read more >>

Asian J Transfus Sci (Asian journal of transfusion science)
[2018, 12(1):42-45]

Cited: 1 time

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