Full Text Journal Articles by
Author Antje M K Thamm


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Solution of the multistep pathway for assembly of corynanthean, strychnos, iboga, and aspidosperma monoterpenoid indole alkaloids from 19E-geissoschizine.

Yang Qu, Michael E A M Easson, Razvan Simionescu, Josef Hajicek, Antje M K Thamm, Vonny Salim, Vincenzo De Luca,

Monoterpenoid indole alkaloids (MIAs) possess a diversity of alkaloid skeletons whose biosynthesis is poorly understood. A bioinformatic search of candidate genes, combined with their virus-induced gene silencing, targeted MIA profiling and in vitro/in vivo pathway reconstitution identified and functionally characterized six genes as well as a seventh enzyme reaction required ... Read more >>

Proc Natl Acad Sci U S A (Proceedings of the National Academy of Sciences of the United States of America)
[2018, 115(12):3180-3185]

Cited: 9 times

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Geissoschizine synthase controls flux in the formation of monoterpenoid indole alkaloids in a Catharanthus roseus mutant.

Yang Qu, Antje M K Thamm, Matthew Czerwinski, Sayaka Masada, Kyung Hee Kim, Graham Jones, Ping Liang, Vincenzo De Luca,

A Catharanthus roseus mutant accumulates high levels of ajmalicine at the expense of catharanthine and vindoline. The altered chemistry depends on increased expression and biochemical activities of strictosidine β-glucosidase and ajmalicine synthase activities and reduced expression and biochemical activity of geissoschizine synthase. The Madagascar periwinkle [Catharanthus roseus (L.) G. Don] ... Read more >>

Planta (Planta)
[2018, 247(3):625-634]

Cited: 2 times

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A tabersonine 3-reductase Catharanthus roseus mutant accumulates vindoline pathway intermediates.

Alison Edge, Yang Qu, Michael L A E Easson, Antje M K Thamm, Kyung Hee Kim, Vincenzo De Luca,

Monoterpenoid indole alkaloids (MIAs) have remarkable biological properties that have led to their medical uses for a variety of human diseases. Mutagenesis has been used to generate plants with new alkaloid profiles and a useful screen for rapid comparison of MIA profiles is described. The MIA mutants identified are useful ... Read more >>

Planta (Planta)
[2018, 247(1):155-169]

Cited: 3 times

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'Nothing of chemistry disappears in biology': the Top 30 damage-prone endogenous metabolites.

Claudia Lerma-Ortiz, James G Jeffryes, Arthur J L Cooper, Thomas D Niehaus, Antje M K Thamm, Océane Frelin, Thomas Aunins, Oliver Fiehn, Valérie de Crécy-Lagard, Christopher S Henry, Andrew D Hanson,

Many common metabolites are intrinsically unstable and reactive, and hence prone to chemical (i.e. non-enzymatic) damage in vivo Although this fact is widely recognized, the purely chemical side-reactions of metabolic intermediates can be surprisingly hard to track down in the literature and are often treated in an unprioritized case-by-case way. Moreover, ... Read more >>

Biochem Soc Trans (Biochemical Society transactions)
[2016, 44(3):961-971]

Cited: 23 times

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Proteins of Unknown Biochemical Function: A Persistent Problem and a Roadmap to Help Overcome It.

Thomas D Niehaus, Antje M K Thamm, Valérie de Crécy-Lagard, Andrew D Hanson,

The number of sequenced genomes is rapidly increasing, but functional annotation of the genes in these genomes lags far behind. Even in Arabidopsis (Arabidopsis thaliana), only approximately 40% of enzyme- and transporter-encoding genes have credible functional annotations, and this number is even lower in nonmodel plants. Functional characterization of unknown ... Read more >>

Plant Physiol. (Plant physiology)
[2015, 169(3):1436-1442]

Cited: 15 times

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A pair of tabersonine 16-hydroxylases initiates the synthesis of vindoline in an organ-dependent manner in Catharanthus roseus.

Sébastien Besseau, Franziska Kellner, Arnaud Lanoue, Antje M K Thamm, Vonny Salim, Bernd Schneider, Fernando Geu-Flores, René Höfer, Grégory Guirimand, Anthony Guihur, Audrey Oudin, Gaëlle Glevarec, Emilien Foureau, Nicolas Papon, Marc Clastre, Nathalie Giglioli-Guivarc'h, Benoit St-Pierre, Danièle Werck-Reichhart, Vincent Burlat, Vincenzo De Luca, Sarah E O'Connor, Vincent Courdavault,

Hydroxylation of tabersonine at the C-16 position, catalyzed by tabersonine 16-hydroxylase (T16H), initiates the synthesis of vindoline that constitutes the main alkaloid accumulated in leaves of Catharanthus roseus. Over the last decade, this reaction has been associated with CYP71D12 cloned from undifferentiated C. roseus cells. In this study, we isolated ... Read more >>

Plant Physiol. (Plant physiology)
[2013, 163(4):1792-1803]

Cited: 30 times

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Functional characterization of amyrin synthase involved in ursolic acid biosynthesis in Catharanthus roseus leaf epidermis.

Fang Yu, Antje M K Thamm, Darwin Reed, Nemesio Villa-Ruano, Alfonso Lara Quesada, Edmundo Lozoya Gloria, Patrick Covello, Vincenzo De Luca,

Catharanthus roseus accumulates high levels of the pentacyclic triterpene, ursolic acid, as a component of its wax exudate on the leaf surface. Bioinformatic analyses of transcripts derived from the leaf epidermis provide evidence for the specialized role of this tissue in the biosynthesis of ursolic acid. Cloning and functional expression ... Read more >>

Phytochemistry (Phytochemistry)
[2013, 91:122-127]

Cited: 9 times

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Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum.

Katharina Caesar, Antje M K Thamm, Janika Witthöft, Kirstin Elgass, Peter Huppenberger, Christopher Grefen, Jakub Horak, Klaus Harter,

Cytokinins are hormones that are involved in various processes of plant growth and development. The model of cytokinin signalling starts with hormone perception through membrane-localized histidine kinase receptors. Although the biochemical properties and functions of these receptors have been extensively studied, there is no solid proof of their subcellular localization. ... Read more >>

J. Exp. Bot. (Journal of experimental botany)
[2011, 62(15):5571-5580]

Cited: 68 times

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