Full Text Journal Articles by
Author Angelika Lehner


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The Secretion of Toxins and Other Exoproteins of Cronobacter: Role in Virulence, Adaption, and Persistence.

Hyein Jang, Gopal R Gopinath, Athmanya Eshwar, Shabarinath Srikumar, Scott Nguyen, Jayanthi Gangiredla, Isha R Patel, Samantha B Finkelstein, Flavia Negrete, JungHa Woo, YouYoung Lee, Séamus Fanning, Roger Stephan, Ben D Tall, Angelika Lehner,

: Cronobacter species are considered an opportunistic group of foodborne pathogenic bacteria capable of causing both intestinal and systemic human disease. This review describes common virulence themes shared among the seven Cronobacter species and describes multiple exoproteins secreted by Cronobacter, many of which are bacterial toxins that may play a ... Read more >>

Microorganisms (Microorganisms)
[2020, 8(2):]

Cited: 0 times

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Molecular characterisation of multi-drug resistant Escherichia coli of bovine origin.

João Anes, Scott V Nguyen, Athmanya K Eshwar, Evonne McCabe, Guerrino Macori, Daniel Hurley, Angelika Lehner, Séamus Fanning,

Antimicrobial resistance reported in bacteria of animal origin is considered a major challenge to veterinary public health. In this study, the genotypic and phenotypic characterisation of twelve Escherichia coli isolates of bovine origin is reported. Twelve bacterial isolates of animal origin were selected from a previous study based on their ... Read more >>

Vet. Microbiol. (Veterinary microbiology)
[2020, 242:108566]

Cited: 0 times

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Whole-Genome Sequencing-Based Characterization of 100 Listeria monocytogenes Isolates Collected from Food Processing Environments over a Four-Year Period.

Daniel Hurley, Laura Luque-Sastre, Craig T Parker, Steven Huynh, Athmanya K Eshwar, Scott V Nguyen, Nicholas Andrews, Alexandra Moura, Edward M Fox, Kieran Jordan, Angelika Lehner, Roger Stephan, Séamus Fanning,

Listeria monocytogenes is frequently found in foods and processing facilities, where it can persist, creating concerns for the food industry. Its ability to survive under a wide range of environmental conditions enhances the potential for cross-contamination of the final food products, leading to possible outbreaks of listeriosis. In this study, ... Read more >>

mSphere (mSphere)
[2019, 4(4):]

Cited: 1 time

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RNA Sequencing-Based Transcriptional Overview of Xerotolerance in Cronobacter sakazakii SP291.

Shabarinath Srikumar, Yu Cao, Qiongqiong Yan, Koenraad Van Hoorde, Scott Nguyen, Shane Cooney, Gopal R Gopinath, Ben D Tall, Sathesh K Sivasankaran, Angelika Lehner, Roger Stephan, Séamus Fanning,

Cronobacter sakazakii is a xerotolerant neonatal pathogen epidemiologically linked to powdered infant food formula, often resulting in high mortality rates. Here, we used transcriptome sequencing (RNA-seq) to provide transcriptional insights into the survival of C. sakazakii in desiccated conditions. Our RNA-seq data show that about 22% of the total C. ... Read more >>

Appl. Environ. Microbiol. (Applied and environmental microbiology)
[2019, 85(3):]

Cited: 2 times

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Draft genomes of Cronobacter sakazakii strains isolated from dried spices bring unique insights into the diversity of plant-associated strains.

Hyein Jang, Jungha Woo, Youyoung Lee, Flavia Negrete, Samantha Finkelstein, Hannah R Chase, Nicole Addy, Laura Ewing, Junia Jean Gilles Beaubrun, Isha Patel, Jayanthi Gangiredla, Athmanya Eshwar, Ziad W Jaradat, Kunho Seo, Srikumar Shabarinath, Séamus Fanning, Roger Stephan, Angelika Lehner, Ben D Tall, Gopal R Gopinath,

Cronobacter sakazakii is a Gram-negative opportunistic pathogen that causes life- threatening infantile infections, such as meningitis, septicemia, and necrotizing enterocolitis, as well as pneumonia, septicemia, and urinary tract and wound infections in adults. Here, we report 26 draft genome sequences of C. sakazakii, which were obtained from dried spices from ... Read more >>

Stand Genomic Sci (Standards in genomic sciences)
[2018, 13:35]

Cited: 2 times

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Interaction of matrix metalloproteinase-9 and Zpx in Cronobacter turicensis LMG 23827T mediated infections in the zebrafish model.

Athmanya Konegadde Eshwar, Nina Wolfrum, Roger Stephan, Séamus Fanning, Angelika Lehner,

Bacteria belonging to the genus Cronobacter have been recognized as causative agents of life-threatening systemic infections primarily in premature and low-birthweight neonates. Validation of putative bacterial virulence components as well as host factors potentially involved in the response to infection has been hampered in the past by the availability of ... Read more >>

Cell. Microbiol. (Cellular microbiology)
[2018, 20(11):e12888]

Cited: 1 time

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Whole-Genome Sequences of Cronobacter sakazakii Isolates Obtained from Foods of Plant Origin and Dried-Food Manufacturing Environments.

Hyein Jang, Nicole Addy, Laura Ewing, Junia Jean-Gilles Beaubrun, YouYoung Lee, JungHa Woo, Flavia Negrete, Samantha Finkelstein, Ben D Tall, Angelika Lehner, Athmanya Eshwar, Gopal R Gopinath,

Here, we present draft genome sequences of 29 Cronobacter sakazakii isolates obtained from foods of plant origin and dried-food manufacturing facilities. Assemblies and annotations resulted in genome sizes ranging from 4.3 to 4.5 Mb and 3,977 to 4,256 gene-coding sequences with G+C contents of ∼57.0%. ... Read more >>

Genome Announc (Genome announcements)
[2018, 6(15):]

Cited: 2 times

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Complete and Assembled Genome Sequence of Salmonella enterica subsp. enterica Serotype Senftenberg N17-509, a Strain Lacking Salmonella Pathogen Island 1.

Marc J A Stevens, Katrin Zurfluh, Denise Althaus, Sabrina Corti, Angelika Lehner, Roger Stephan,

The genome of Salmonella enterica subsp. enterica serotype Senftenberg N17-509, a strain isolated from desiccated coconut, was sequenced using single-molecule real-time sequencing. It consists of a 5.1-Mbp chromosome and a 29-kb linear plasmid. ... Read more >>

Genome Announc (Genome announcements)
[2018, 6(12):]

Cited: 1 time

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Genomic characterization of malonate positive Cronobacter sakazakii serotype O:2, sequence type 64 strains, isolated from clinical, food, and environment samples.

Gopal R Gopinath, Hannah R Chase, Jayanthi Gangiredla, Athmanya Eshwar, Hyein Jang, Isha Patel, Flavia Negrete, Samantha Finkelstein, Eunbi Park, TaeJung Chung, YeonJoo Yoo, JungHa Woo, YouYoung Lee, Jihyeon Park, Hyerim Choi, Seungeun Jeong, Soyoung Jun, Mijeong Kim, Chaeyoon Lee, HyeJin Jeong, Séamus Fanning, Roger Stephan, Carol Iversen, Felix Reich, Günter Klein, Angelika Lehner, Ben D Tall,

Malonate utilization, an important differential trait, well recognized as being possessed by six of the seven Cronobacter species is thought to be largely absent in Cronobacter sakazakii (Csak). The current study provides experimental evidence that confirms the presence of a malonate utilization operon in 24 strains of sequence type (ST) ... Read more >>

Gut Pathog (Gut pathogens)
[2018, 10:11]

Cited: 2 times

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Comparative Genomic Characterization of the Highly Persistent and Potentially Virulent Cronobacter sakazakii ST83, CC65 Strain H322 and Other ST83 Strains.

Hannah R Chase, Gopal R Gopinath, Athmanya K Eshwar, Andrea Stoller, Claudia Fricker-Feer, Jayanthi Gangiredla, Isha R Patel, Hediye N Cinar, HyeJin Jeong, ChaeYoon Lee, Flavia Negrete, Samantha Finkelstein, Roger Stephan, Ben D Tall, Angelika Lehner,

Cronobacter (C.) sakazakii is an opportunistic pathogen and has been associated with serious infections with high mortality rates predominantly in pre-term, low-birth weight and/or immune compromised neonates and infants. Infections have been epidemiologically linked to consumption of intrinsically and extrinsically contaminated lots of reconstituted powdered infant formula (PIF), thus contamination ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2017, 8:1136]

Cited: 6 times

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Draft Genome Sequence of Cronobacter sakazakii GP1999, Sequence Type 145, an Epiphytic Isolate Obtained from the Tomato's Rhizoplane/Rhizosphere Continuum.

Hannah R Chase, Leo Eberl, Roger Stephan, HyeJin Jeong, Chaeyoon Lee, Samantha Finkelstein, Flavia Negrete, Jayanthi Gangiredla, Isha Patel, Ben D Tall, Gopal R Gopinath, Angelika Lehner,

We present here the draft genome of Cronobacter sakazakii GP1999, a sequence type 145 strain isolated from the rhizosphere of tomato plants. Assembly and annotation of the genome resulted in a genome of 4,504,670 bp in size, with 4,148 coding sequences, and a GC content of 56.8%. ... Read more >>

Genome Announc (Genome announcements)
[2017, 5(31):]

Cited: 2 times

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Local Outbreak of Listeria monocytogenes Serotype 4b Sequence Type 6 Due to Contaminated Meat Pâté.

Denise Althaus, Marco Jermini, Petra Giannini, Gladys Martinetti, Danuta Reinholz, Magdalena Nüesch-Inderbinen, Angelika Lehner, Roger Stephan,

In January and February 2016, five cases of confirmed and two cases of probable infection due to Listeria monocytogenes serotype 4b, sequence type (ST) 6 belonging to a single pulsed-field gel electrophoresis pulsotype pattern were registered in a region of southern Switzerland. L. monocytogenes was detected in blood samples (four ... Read more >>

Foodborne Pathog. Dis. (Foodborne pathogens and disease)
[2017, 14(4):219-222]

Cited: 5 times

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Use of a Pan-Genomic DNA Microarray in Determination of the Phylogenetic Relatedness among Cronobacter spp. and Its Use as a Data Mining Tool to Understand Cronobacter Biology.

Ben D Tall, Jayanthi Gangiredla, Christopher J Grim, Isha R Patel, Scott A Jackson, Mark K Mammel, Mahendra H Kothary, Venugopal Sathyamoorthy, Laurenda Carter, Séamus Fanning, Carol Iversen, Franco Pagotto, Roger Stephan, Angelika Lehner, Jeffery Farber, Qiong Q Yan, Gopal R Gopinath,

Cronobacter (previously known as Enterobacter sakazakii) is a genus of Gram-negative, facultatively anaerobic, oxidase-negative, catalase-positive, rod-shaped bacteria of the family Enterobacteriaceae. These organisms cause a variety of illnesses such as meningitis, necrotizing enterocolitis, and septicemia in neonates and infants, and urinary tract, wound, abscesses or surgical site infections, septicemia, and ... Read more >>

Microarrays (Basel) (Microarrays (Basel, Switzerland))
[2017, 6(1):]

Cited: 2 times

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Linking Genomo- and Pathotype: Exploiting the Zebrafish Embryo Model to Investigate the Divergent Virulence Potential among Cronobacter spp.

Athmanya K Eshwar, Ben D Tall, Jayanthi Gangiredla, Gopal R Gopinath, Isha R Patel, Stephan C F Neuhauss, Roger Stephan, Angelika Lehner,

Bacteria belonging to the genus Cronobacter have been recognized as causative agents of life-threatening systemic infections primarily in premature, low-birth weight and immune-compromised neonates. Apparently not all Cronobacter species are linked to infantile infections and it has been proposed that virulence varies among strains. Whole genome comparisons and in silico ... Read more >>

PLoS ONE (PloS one)
[2016, 11(6):e0158428]

Cited: 4 times

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The DSF type quorum sensing signalling system RpfF/R regulates diverse phenotypes in the opportunistic pathogen Cronobacter.

Angela Suppiger, Athmanya Konegadde Eshwar, Roger Stephan, Volkhard Kaever, Leo Eberl, Angelika Lehner,

Several bacterial pathogens produce diffusible signal factor (DSF)-type quorum sensing (QS) signals to control biofilm formation and virulence. Previous work showed that in Burkholderia cenocepacia the RpfFBc/RpfR system is involved in sensing and responding to DSF signals and that this signal/sensor gene pair is highly conserved in several bacterial species ... Read more >>

Sci Rep (Scientific reports)
[2016, 6:18753]

Cited: 10 times

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The response of foodborne pathogens to osmotic and desiccation stresses in the food chain.

Catherine M Burgess, Andrea Gianotti, Nadia Gruzdev, John Holah, Susanne Knøchel, Angelika Lehner, Edyta Margas, Stephan Schmitz Esser, Shlomo Sela Saldinger, Odile Tresse,

In combination with other strategies, hyperosmolarity and desiccation are frequently used by the food processing industry as a means to prevent bacterial proliferation, and particularly that of foodborne pathogens, in food products. However, it is increasingly observed that bacteria, including human pathogens, encode mechanisms to survive and withstand these stresses. ... Read more >>

Int. J. Food Microbiol. (International journal of food microbiology)
[2016, 221:37-53]

Cited: 25 times

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Evaluation of zebrafish as a model to study the pathogenesis of the opportunistic pathogen Cronobacter turicensis.

Alexander Fehr, Athmanya K Eshwar, Stephan C F Neuhauss, Maja Ruetten, Angelika Lehner, Lloyd Vaughan,

Bacteria belonging to the genus Cronobacter spp. have been recognized as causative agents of life-threatening systemic infections, primarily in premature, low-birth weight and/or immune-compromised neonates. Knowledge remains scarce regarding the underlying molecular mechanisms of disease development. In this study, we evaluated the use of a zebrafish model to study the ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2015, 4(5):e29]

Cited: 8 times

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Development of a Custom-Designed, Pan Genomic DNA Microarray to Characterize Strain-Level Diversity among Cronobacter spp.

Ben Davies Tall, Jayanthi Gangiredla, Gopal R Gopinath, Qiongqiong Yan, Hannah R Chase, Boram Lee, Seongeun Hwang, Larisa Trach, Eunbi Park, YeonJoo Yoo, TaeJung Chung, Scott A Jackson, Isha R Patel, Venugopal Sathyamoorthy, Monica Pava-Ripoll, Michael L Kotewicz, Laurenda Carter, Carol Iversen, Franco Pagotto, Roger Stephan, Angelika Lehner, Séamus Fanning, Christopher J Grim,

Cronobacter species cause infections in all age groups; however neonates are at highest risk and remain the most susceptible age group for life-threatening invasive disease. The genus contains seven species:Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter turicensis, Cronobacter muytjensii, Cronobacter dublinensis, Cronobacter universalis, and Cronobacter condimenti. Despite an abundance of published genomes ... Read more >>

Front Pediatr (Frontiers in pediatrics)
[2015, 3:36]

Cited: 9 times

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Influence of FkpA variants on survival and replication of Cronobacter spp. in human macrophages.

Athmanya Konegadde Eshwar, Taurai Tasara, Roger Stephan, Angelika Lehner,

Members of the genus Cronobacter are responsible for cases of meningitis and bacteremia with high fatality rates in neonates. Macrophage uptake of invading microbes is an innate process, and it has been proposed that macrophage infectivity potentiator (Mip) like proteins enhance the ability of pathogens to survive within macrophages. Cronobacter ... Read more >>

Res. Microbiol. (Research in microbiology)
[2015, 166(3):186-195]

Cited: 6 times

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A Cronobacter turicensis O1 antigen-specific monoclonal antibody inhibits bacterial motility and entry into epithelial cells.

Kristina Schauer, Angelika Lehner, Richard Dietrich, Ina Kleinsteuber, Rocío Canals, Katrin Zurfluh, Kerstin Weiner, Erwin Märtlbauer,

Cronobacter turicensis is an opportunistic foodborne pathogen that can cause a rare but sometimes lethal infection in neonates. Little is known about the virulence mechanisms and intracellular lifestyle of this pathogen. In this study, we developed an IgG monoclonal antibody (MAb; MAb 2G4) that specifically recognizes the O1 antigen of ... Read more >>

Infect. Immun. (Infection and immunity)
[2015, 83(3):876-887]

Cited: 4 times

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Characterization of Listeria monocytogenes strains isolated during 2011-2013 from human infections in Switzerland.

Denise Althaus, Angelika Lehner, Sylvain Brisse, Mylène Maury, Taurai Tasara, Roger Stephan,

Listeria monocytogenes, an emerging foodborne pathogen, can cause in the population at risk severe infections that are associated with high case fatality rates. A total of 93 L. monocytogenes strains isolated from different patients in Switzerland from July 2011 to September 2013 were further characterized. Septicemia was reported for 74.2% ... Read more >>

Foodborne Pathog. Dis. (Foodborne pathogens and disease)
[2014, 11(10):753-758]

Cited: 32 times

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Presence of AmpC beta-lactamases, CSA-1, CSA-2, CMA-1, and CMA-2 conferring an unusual resistance phenotype in Cronobacter sakazakii and Cronobacter malonaticus.

Andrea Müller, Herbert Hächler, Roger Stephan, Angelika Lehner,

Here we describe the presence of two very similar but unusual variants of AmpC cephalosporinase in each Cronobacter sakazakii and C. malonaticus isolates conferring resistance exclusively to first generation cephalosporins. During a survey on the antibiotic resistance patterns of C. sakazakii and C. malonaticus strains isolated from a milk powder ... Read more >>

Microb. Drug Resist. (Microbial drug resistance (Larchmont, N.Y.))
[2014, 20(4):275-280]

Cited: 7 times

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Outbreak of Staphylococcal food poisoning due to SEA-producing Staphylococcus aureus.

Sophia Johler, Petra S Tichaczek-Dischinger, Jörg Rau, Henna-Maria Sihto, Angelika Lehner, Maja Adam, Roger Stephan,

In 2008, 150 people gathered for a wedding celebration in Baden-Württemberg, Germany. Three hours after ingestion of a variety of foods including pancakes filled with minced chicken, several guests exhibited symptoms of acute gastroenteritis such as vomiting, diarrhea, fever, and ague. Twelve guests were reported to have fallen ill, with ... Read more >>

Foodborne Pathog. Dis. (Foodborne pathogens and disease)
[2013, 10(9):777-781]

Cited: 17 times

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Genetic diversity of Cronobacter sakazakii isolates collected from a Swiss infant formula production facility.

Andrea Müller, Roger Stephan, Claudia Fricker-Feer, Angelika Lehner,

In this study, 141 Cronobacter isolates that were collected based on a hygienic monitoring program performed in a powdered infant formula production facility in Switzerland between September 2011 and October 2012 were further characterized. Isolates were identified to the species level by molecular methods, and strains of Cronobacter sakazakii were ... Read more >>

J. Food Prot. (Journal of food protection)
[2013, 76(5):883-887]

Cited: 23 times

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Pan-genome analysis of the emerging foodborne pathogen Cronobacter spp. suggests a species-level bidirectional divergence driven by niche adaptation.

Christopher J Grim, Michael L Kotewicz, Karen A Power, Gopal Gopinath, Augusto A Franco, Karen G Jarvis, Qiong Q Yan, Scott A Jackson, Venugopal Sathyamoorthy, Lan Hu, Franco Pagotto, Carol Iversen, Angelika Lehner, Roger Stephan, Séamus Fanning, Ben D Tall,

BACKGROUND: Members of the genus Cronobacter are causes of rare but severe illness in neonates and preterm infants following the ingestion of contaminated infant formula. Seven species have been described and two of the species genomes were subsequently published. In this study, we performed comparative genomics on eight strains of ... Read more >>

BMC Genomics (BMC genomics)
[2013, 14:366]

Cited: 30 times

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