Full Text Journal Articles by
Author Alexandre J da Silva

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Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices.

Alexandre D T Costa, Thiago Jacomasso, Elaine C Mattos, Aline B Farias, Rita C P Rampazzo, Rebeka S Pinto, Walleyd Tassi, Maria Aparecida M Marciano, Vera Lucia Pereira-Chioccola, Helen R Murphy, Alexandre J da Silva, Marco A Krieger,

Foodborne outbreaks caused by parasites have long been a public health issue. Among the available contamination detection methods, qPCR is one of the most sensitive and specific. However, it can be cumbersome and error-prone, if used by unexperienced users. Moreover, qPCR reagents usually require freezer temperatures for transportation and storage. ... Read more >>

Food Waterborne Parasitol (Food and waterborne parasitology)
[2021, 22:e00111]

Cited: 0 times

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Detection of Cyclospora cayetanensis in produce irrigation and wash water using large-volume sampling techniques.

Amy M Kahler, Mia C Mattioli, Alexandre J da Silva, Vincent Hill,

The recent increase of reported cyclosporiasis outbreaks associated with fresh produce has highlighted the need for understanding environmental transmission of <i>Cyclospora cayetanensis</i> in agricultural settings and facilities. Conducting such environmental investigations necessitates robust sample collection and analytical methods to detect <i>C. cayetanensis</i> in water samples. This study evaluated three sample ... Read more >>

Food Waterborne Parasitol (Food and waterborne parasitology)
[2021, 22:e00110]

Cited: 0 times

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Angiostrongylus spp. (Nematoda; Metastrongyloidea) of global public health importance.

Alexandre J da Silva, Alessandra L Morassutti,

Human angiostrongyliasis is an important foodborne zoonosis, caused by the infection with Angiostrongylus costaricensis and Angiostrongylus cantonensis. These two species have a significant public health impact in different areas of the world. Angiostrongyliasis is re-emerging and expanding to urban settings rising significant concerns regarding the control of these infections. This ... Read more >>

Res Vet Sci (Research in veterinary science)
[2021, 135:397-403]

Cited: 1 time

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Dead-End Ultrafiltration and DNA-Based Methods for Detection of Cyclospora cayetanensis in Agricultural Water.

Mauricio Durigan, Helen R Murphy, Alexandre J da Silva,

<i>Cyclospora cayetanensis</i> is a protozoan parasite that causes foodborne and waterborne diarrheal illness outbreaks worldwide. Most of these outbreaks are associated with the consumption of fresh produce. Sensitive and specific methods to detect <i>C. cayetanensis</i> in agricultural water are needed to identify the parasite in agricultural water used to irrigate ... Read more >>

Appl Environ Microbiol (Applied and environmental microbiology)
[2020, 86(23):]

Cited: 1 time

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Duplex Real-Time PCR Assay for Clinical Differentiation of Onchocerca lupi and Onchocerca volvulus.

Marcos de Almeida, Fernanda S Nascimento, Blaine A Mathison, Henry Bishop, Richard S Bradbury, Vitaliano A Cama, Alexandre J da Silva,

In the United States and Europe, human onchocerciasis is a rare disease caused by zoonotic or anthropophilic parasites in the genus <i>Onchocerca</i>. The zoonotic species identified in focal areas of Europe and United States is <i>Onchocerca lupi</i>, and <i>Onchocerca volvulus</i>, the anthroponotic species, may be found among people who had ... Read more >>

Am J Trop Med Hyg (The American journal of tropical medicine and hygiene)
[2020, 103(4):1556-1562]

Cited: 1 time

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Proof of Concept for a Portable Platform for Molecular Diagnosis of Tropical Diseases: On-Chip Ready-to-Use Real-Time Quantitative PCR for Detection of Trypanosoma cruzi or Plasmodium spp.

Rita C P Rampazzo, Ana Cláudia Graziani, Keren K Leite, Jhully Anni Surdi, Cheysa A Biondo, Maykon L N Costa, Thiago Jacomasso, Marco Cereda, Marco De Fazio, Marco A Bianchessi, Otacílio C Moreira, Constança Britto, Joana D N Costa, Viviane M Góes, Alexandre J da Silva, Marco A Krieger, Alexandre D T Costa,

Although molecular diagnostics is well established in clinical laboratories, its full potential has not been extended to field settings. Typically, diagnostic real-time quantitative PCR (qPCR) reagents require temperature-controlled transportation and storage. Furthermore, thermocyclers are bulky and fragile, requiring good infrastructure for optimal operation. These major hurdles strongly limit use of ... Read more >>

J Mol Diagn (The Journal of molecular diagnostics : JMD)
[2019, 21(5):839-851]

Cited: 5 times

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Evaluation of the U.S. Food and Drug Administration validated method for detection of Cyclospora cayetanensis in high-risk fresh produce matrices and a method modification for a prepared dish

Sonia Almeria, Alexandre J da Silva, Tyann Blessington, Tami Craig Cloyd, Hediye Nese Cinar, Mauricio Durigan, Helen R Murphy,

The performance of the U.S. Food and Drug Administration (FDA) validated method for regulatory detection of Cyclospora cayetanensis in leafy greens and berries was evaluated in additional high-risk fresh produce items and in a dish prepared with these produce commodities. The method was robust and reproducible in basil, parsley, shredded ... Read more >>

Food Microbiol (Food microbiology)
[2018, 76:497-503]

Cited: 3 times

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Peptidyl-prolyl cis-trans isomerase A - A novel biomarker of multi-episodic (recurrent) ocular toxoplasmosis.

Jordan Isenberg, Makan Golizeh, Rubens N Belfort, Alexandre J da Silva, Miguel N Burnier, Momar Ndao,

Ocular toxoplasmosis (OT) is the most common etiology of posterior uveitis. The high incidence of macular scarring associated with OT is a leading cause of visual morbidity. Serum biomarkers of the disease would aid in its diagnosis. This study sought, for the first time, to elucidate serum biomarkers for OT ... Read more >>

Exp Eye Res (Experimental eye research)
[2018, 177:104-111]

Cited: 1 time

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Evaluation of the U.S. Food and Drug Administration validated method for detection of Cyclospora cayetanensis in high-risk fresh produce matrices and a method modification for a prepared dish.

Sonia Almeria, Alexandre J da Silva, Tyann Blessington, Tami Craig Cloyd, Hediye Nese Cinar, Mauricio Durigan, Helen R Murphy,

The performance of the U.S. Food and Drug Administration (FDA) validated method for regulatory detection of Cyclospora cayetanensis in leafy greens and berries was evaluated in additional high-risk fresh produce items and in a dish prepared with these produce commodities. The method was robust and reproducible in basil, parsley, shredded ... Read more >>

Food Microbiol (Food microbiology)
[2018, 76:497-503]

Cited: 3 times

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Molecular detection of Cyclospora cayetanensis in human stool specimens using UNEX-based DNA extraction and real-time PCR.

Yvonne Qvarnstrom, Theresa Benedict, Paula L Marcet, Ryan E Wiegand, Barbara L Herwaldt, Alexandre J da Silva,

Cyclospora cayetanensis is a coccidian parasite associated with diarrheal illness. In the USA, foodborne outbreaks of cyclosporiasis have been documented almost every year since the mid-1990s. The typical approach used to identify this parasite in human stools is an examination of acid-fast-stained smears under bright-field microscopy. UV fluorescence microscopy of ... Read more >>

Parasitology (Parasitology)
[2018, 145(7):865-870]

Cited: 10 times

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Evaluation of an Improved U.S. Food and Drug Administration Method for the Detection of Cyclospora cayetanensis in Produce Using Real-Time PCR.

Helen R Murphy, Seulgi Lee, Alexandre J da Silva,

Cyclospora cayetanensis is a protozoan parasite that causes human diarrheal disease associated with the consumption of fresh produce or water contaminated with C. cayetanensis oocysts. In the United States, foodborne outbreaks of cyclosporiasis have been linked to various types of imported fresh produce, including cilantro and raspberries. An improved method ... Read more >>

J Food Prot (Journal of food protection)
[2017, 80(7):1133-1144]

Cited: 13 times

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Interlaboratory validation of an improved method for detection of Cyclospora cayetanensis in produce using a real-time PCR assay.

Helen R Murphy, Hediye Nese Cinar, Gopal Gopinath, Kathy E Noe, Lacresha D Chatman, Nancy E Miranda, June H Wetherington, Jason Neal-McKinney, Gabrielle S Pires, Elizabeth Sachs, Kristopher J Stanya, Cynthia L Johnson, Fernanda S Nascimento, Monica Santin, Aleksey Molokin, Mansour Samadpour, Harish Janagama, Amy Kahler, Candace Miller, Alexandre J da Silva,

A collaborative validation study was performed to evaluate the performance of a new U.S. Food and Drug Administration method developed for detection of the protozoan parasite, Cyclospora cayetanensis, on cilantro and raspberries. The method includes a sample preparation step in which oocysts are recovered from produce using an enhanced produce ... Read more >>

Food Microbiol (Food microbiology)
[2018, 69:170-178]

Cited: 7 times

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Three Cases of Neurologic Syndrome Caused by Donor-Derived Microsporidiosis.

Rachel M Smith, Atis Muehlenbachs, Joanna Schaenmann, Sanjiv Baxi, Sophia Koo, Dianna Blau, Peter Chin-Hong, Anna R Thorner, Matthew J Kuehnert, Kristina Wheeler, Alexis Liakos, Jonathan W Jackson, Theresa Benedict, Alexandre J da Silva, Jana M Ritter, Dominique Rollin, Maureen Metcalfe, Cynthia S Goldsmith, Govinda S Visvesvara, Sridhar V Basavaraju, Sherif Zaki,

In April 2014, a kidney transplant recipient in the United States experienced headache, diplopia, and confusion, followed by neurologic decline and death. An investigation to evaluate the possibility of donor-derived infection determined that 3 patients had received 4 organs (kidney, liver, heart/kidney) from the same donor. The liver recipient experienced ... Read more >>

Emerg Infect Dis (Emerging infectious diseases)
[2017, 23(3):387-395]

Cited: 3 times

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Detection and Differentiation of Leishmania spp. in Clinical Specimens by Use of a SYBR Green-Based Real-Time PCR Assay.

Marcos E de Almeida, Ozgur Koru, Francis Steurer, Barbara L Herwaldt, Alexandre J da Silva,

Leishmaniasis in humans is caused by Leishmania spp. in the subgenera Leishmania and Viannia Species identification often has clinical relevance. Until recently, our laboratory relied on conventional PCR amplification of the internal transcribed spacer 2 (ITS2) region (ITS2-PCR) followed by sequencing analysis of the PCR product to differentiate Leishmania spp. ... Read more >>

J Clin Microbiol (Journal of clinical microbiology)
[2017, 55(1):281-290]

Cited: 12 times

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Cross-reactivity of the 31 kDa antigen of Angiostrongylus cantonensis - Dealing with the immunodiagnosis of meningoencephalitis.

Alessandra L Morassutti, Lisa N Rascoe, Sukwan Handali, Alexandre J DA Silva, Patricia P Wilkins, Carlos Graeff-Teixeira,

The primary causative agent of eosinophilic meningoencephalitis (EoM) in endemic regions is the nematode Angiostrongylus cantonensis. The occurrence of EoM was previously restricted to countries in Southeast Asia and the Pacific Islands; however, more recently, it has been reported from other regions, including Brazil. The commonly used diagnosis is detection ... Read more >>

Parasitology (Parasitology)
[2017, 144(4):459-463]

Cited: 1 time

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Evaluation of library preparation methods for Illumina next generation sequencing of small amounts of DNA from foodborne parasites.

Fernanda S Nascimento, Yuping Wei-Pridgeon, Michael J Arrowood, Delynn Moss, Alexandre J da Silva, Eldin Talundzic, Yvonne Qvarnstrom,

Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input DNA was in the picogram range. ... Read more >>

J Microbiol Methods (Journal of microbiological methods)
[2016, 130:23-26]

Cited: 9 times

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Transmission of Balamuthia mandrillaris by Organ Transplantation.

Eileen C Farnon, Kenneth E Kokko, Philip J Budge, Chukwuma Mbaeyi, Emily C Lutterloh, Yvonne Qvarnstrom, Alexandre J da Silva, Wun-Ju Shieh, Sharon L Roy, Christopher D Paddock, Rama Sriram, Sherif R Zaki, Govinda S Visvesvara, Matthew J Kuehnert, , Joli Weiss, Ken Komatsu, Richard Manch, Alberto Ramos, Leonor Echeverria, Ann Moore, Phil Zakowski, Michelle Kittleson, Jon Kobashigawa, Jonathan Yoder, Michael Beach, William Mahle, Kirk Kanter, P J Geraghty, Eileen Navarro, Christine Hahn, Shiro Fujita, Jim Stinson, Joel Trachtenberg, Paul Byers, Michele Cheung, Tun Jie, Bruce Kaplan, Rainer Gruessner, Erica Bracamonte, Chad Viscusi, Regino Gonzalez-Peralta, Robert Lawrence, Jonathan Fratkin, Fauzia Butt,

<h4>Background</h4>During 2009 and 2010, 2 clusters of organ transplant-transmitted Balamuthia mandrillaris, a free-living ameba, were detected by recognition of severe unexpected illness in multiple recipients from the same donor.<h4>Methods</h4>We investigated all recipients and the 2 donors through interview, medical record review, and testing of available specimens retrospectively. Surviving recipients were ... Read more >>

Clin Infect Dis (Clinical infectious diseases : an official publication of the Infectious Diseases Society of America)
[2016, 63(7):878-888]

Cited: 9 times

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Amoebic meningoencephalitis and disseminated infection caused by Balamuthia mandrillaris in a Western lowland gorilla (Gorilla gorilla gorilla).

Jenessa L Gjeltema, Brigid Troan, Atis Muehlenbachs, Lindy Liu, Alexandre J Da Silva, Yvonne Qvarnstrom, Jeremy R Tobias, Michael R Loomis, Ryan S De Voe,

CASE DESCRIPTION A 22-year-old male gorilla (Gorilla gorilla gorilla) housed in a zoo was evaluated for signs of lethargy, head-holding, and cervical stiffness followed by development of neurologic abnormalities including signs of depression, lip droop, and tremors. CLINICAL FINDINGS Physical examination under general anesthesia revealed a tooth root abscess and ... Read more >>

J Am Vet Med Assoc (Journal of the American Veterinary Medical Association)
[2016, 248(3):315-321]

Cited: 1 time

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Real-Time Polymerase Chain Reaction Detection of Angiostrongylus cantonensis DNA in Cerebrospinal Fluid from Patients with Eosinophilic Meningitis.

Yvonne Qvarnstrom, Maniphet Xayavong, Ana Cristina Aramburu da Silva, Sarah Y Park, A Christian Whelen, Precilia S Calimlim, Rebecca H Sciulli, Stacey A A Honda, Karen Higa, Paul Kitsutani, Nora Chea, Seng Heng, Stuart Johnson, Carlos Graeff-Teixeira, LeAnne M Fox, Alexandre J da Silva,

Angiostrongylus cantonensis is the most common infectious cause of eosinophilic meningitis. Timely diagnosis of these infections is difficult, partly because reliable laboratory diagnostic methods are unavailable. The aim of this study was to evaluate the usefulness of a real-time polymerase chain reaction (PCR) assay for the detection of A. cantonensis ... Read more >>

Am J Trop Med Hyg (The American journal of tropical medicine and hygiene)
[2016, 94(1):176-181]

Cited: 25 times

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Review of Telemicrobiology.

Daniel D Rhoads, Blaine A Mathison, Henry S Bishop, Alexandre J da Silva, Liron Pantanowitz,

<h4>Context</h4>Microbiology laboratories are continually pursuing means to improve quality, rapidity, and efficiency of specimen analysis in the face of limited resources. One means by which to achieve these improvements is through the remote analysis of digital images. Telemicrobiology enables the remote interpretation of images of microbiology specimens. To date, the ... Read more >>

Arch Pathol Lab Med (Archives of pathology & laboratory medicine)
[2016, 140(4):362-370]

Cited: 6 times

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Performance of a real time PCR for leishmaniasis diagnosis using a L. (L.) infantum hypothetical protein as target in canine samples.

Fabio Antonio Colombo, Vera Lucia Pereira-Chioccola, Cristina da Silva Meira, Gabriela Motoie, Ricardo Gava, Roberto M Hiramoto, Marcos E de Almeida, Alexandre J da Silva, Andre Antonio Cutolo, Ingrid Menz,

Visceral leishmaniasis represents an important public health issue in different parts of the world, requiring that measures be put in place to control the spread of the disease worldwide. The canine leishmaniasis diagnosis is not easy based on clinical signs, since dogs may not develop the infection with recognizable signs. ... Read more >>

Exp Parasitol (Experimental parasitology)
[2015, 157:156-162]

Cited: 5 times

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Spread of the Rat Lungworm (Angiostrongylus cantonensis) in Giant African Land Snails (Lissachatina fulica) in Florida, USA.

Deborah D Iwanowicz, Lakyn R Sanders, W Bane Schill, Maniphet V Xayavong, Alexandre J da Silva, Yvonne Qvarnstrom, Trevor Smith,

The rat lungworm (Angiostrongylus cantonensis) is a parasitic nematode that causes rat lungworm disease. It is the leading cause of eosinophilic meningitis and is a zoonotic health risk. We confirmed the presence of A. cantonensis using species-specific, quantitative PCR in 18 of 50 (36%) giant African land snails (Lissachatina fulica) ... Read more >>

J Wildl Dis (Journal of wildlife diseases)
[2015, 51(3):749-753]

Cited: 9 times

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A linear mitochondrial genome of Cyclospora cayetanensis (Eimeriidae, Eucoccidiorida, Coccidiasina, Apicomplexa) suggests the ancestral start position within mitochondrial genomes of eimeriid coccidia.

Mosun E Ogedengbe, Yvonne Qvarnstrom, Alexandre J da Silva, Michael J Arrowood, John R Barta,

The near complete mitochondrial genome for Cyclospora cayetanensis is 6184 bp in length with three protein-coding genes (Cox1, Cox3, CytB) and numerous lsrDNA and ssrDNA fragments. Gene arrangements were conserved with other coccidia in the Eimeriidae, but the C. cayetanensis mitochondrial genome is not circular-mapping. Terminal transferase tailing and nested ... Read more >>

Int J Parasitol (International journal for parasitology)
[2015, 45(6):361-365]

Cited: 12 times

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The first association of a primary amebic meningoencephalitis death with culturable Naegleria fowleri in tap water from a US treated public drinking water system.

Jennifer R Cope, Raoult C Ratard, Vincent R Hill, Theresa Sokol, Jonathan Jake Causey, Jonathan S Yoder, Gayatri Mirani, Bonnie Mull, Kimberly A Mukerjee, Jothikumar Narayanan, Meggie Doucet, Yvonne Qvarnstrom, Charla N Poole, Olugbenga A Akingbola, Jana M Ritter, Zhenggang Xiong, Alexandre J da Silva, Dawn Roellig, Russell B Van Dyke, Harlan Stern, Lihua Xiao, Michael J Beach,

<h4>Background</h4>Naegleria fowleri is a climate-sensitive, thermophilic ameba found in warm, freshwater lakes and rivers. Primary amebic meningoencephalitis (PAM), which is almost universally fatal, occurs when N. fowleri-containing water enters the nose, typically during swimming, and migrates to the brain via the olfactory nerve. In August 2013, a 4-year-old boy died ... Read more >>

Clin Infect Dis (Clinical infectious diseases : an official publication of the Infectious Diseases Society of America)
[2015, 60(8):e36-42]

Cited: 22 times

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Successful treatment of an adolescent with Naegleria fowleri primary amebic meningoencephalitis.

W Matthew Linam, Mubbasheer Ahmed, Jennifer R Cope, Craig Chu, Govinda S Visvesvara, Alexandre J da Silva, Yvonne Qvarnstrom, Jerril Green,

Naegleria fowleri is a thermophilic, free-living ameba that causes primary amebic meningoencephalitis. The infections are nearly always fatal. We present the third well-documented survivor of this infection in North America. The patient's survival most likely resulted from a variety of factors: early identification and treatment, use of a combination of ... Read more >>

Pediatrics (Pediatrics)
[2015, 135(3):e744-8]

Cited: 35 times

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