Full Text Journal Articles by
Author Alain Xicluna


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Immobilization of arginase and its application in an enzymatic chromatographic column: thermodynamic studies of nor-NOHA/arginase binding and role of the reactive histidine residue.

Teddy Bagnost, Yves-Claude Guillaume, Mireille Thomassin, Jean-François Robert, Alain Berthelot, Alain Xicluna, Claire André,

A biochromatographic approach is developed to measure for the first time changes in enthalpy, heat capacity change and protonation for the binding of nor-NOHA to arginase in a wide temperature range. For this, the arginase enzyme was immobilized on a chromatographic support. It was established that this novel arginase column ... Read more >>

J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (Journal of chromatography. B, Analytical technologies in the biomedical and life sciences)
[2007, 856(1-2):113-120]

Cited: 6 times

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Synthesis and antioxidant activity evaluation of new hexahydropyrimido[5,4-c]quinoline-2,5-diones and 2-thioxohexahydropyrimido[5,4-c]quinoline-5-ones obtained by Biginelli reaction in two steps.

Lhassane Ismaili, Arulraj Nadaradjane, Laurence Nicod, Catherine Guyon, Alain Xicluna, Jean-François Robert, Bernard Refouvelet,

New hexahydropyrimido[5,4-c]quinoline-2,5-diones and 2-thioxohexahydropyrimido[5,4-c]quinoline-5-ones were prepared in two steps from ethyl 4-phenyl-6-methyl-2-oxo tetrahydropyrimidine-5-carboxylates or 4-phenyl-6-methyl-2-thioxotetrahydropyrimidine-5-carboxylates, previously prepared by Biginelli reaction using appropriate aldehyde, urea derivatives and ethyl acetoacetate. Their antioxidant properties were evaluated by two methods: scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and scavenging effect on hydroxyl radicals. The results ... Read more >>

Eur J Med Chem (European journal of medicinal chemistry)
[2008, 43(6):1270-1275]

Cited: 10 times

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Synthesis, structure, and estrogenic activity of 4-amino-3-(2-methylbenzyl)coumarins on human breast carcinoma cells.

Yves Jacquot, Ioanna Laïos, Anny Cleeren, Denis Nonclercq, Laurent Bermont, Bernard Refouvelet, Kamal Boubekeur, Alain Xicluna, Guy Leclercq, Guy Laurent,

A number of coumarins exhibit interesting pharmacological activities and are therefore of therapeutic use. We report here the synthesis and the structural analysis of new N-substituted 4-amino-3-(2-methylbenzyl)coumarins (compounds 8a-8e) that present structural analogies with estrothiazine and 11- or 7-substituted 17beta-estradiol. These derivatives were tested with respect to estrogenic activity on ... Read more >>

Bioorg. Med. Chem. (Bioorganic & medicinal chemistry)
[2007, 15(6):2269-2282]

Cited: 11 times

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Aptamer-oligonucleotide binding studied by capillary electrophoresis: cation effect and separation efficiency.

Claire André, Alain Xicluna, Yves-Claude Guillaume,

Novel features of DNA structure, recognition and discrimination have been recently elucidated through the solution structural characterization of DNA aptamers that bind cofactors, amino acids and peptides with high affinity and specificity. Multidimensional nuclear magnetic resonance methodologies have been successfully applied to solve the solution structures. In this work, it ... Read more >>

Electrophoresis (Electrophoresis)
[2005, 26(17):3247-3255]

Cited: 9 times

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Synthesis of 4-hydroxycoumarin and 2,4-quinolinediol derivatives and evaluation of their effects on the viability of HepG2 cells and human hepatocytes culture.

Bernard Refouvelet, Catherine Guyon, Yves Jacquot, Corinne Girard, Hervé Fein, Françoise Bévalot, Jean-François Robert, Bruno Heyd, Georges Mantion, Lysiane Richert, Alain Xicluna,

We report here the synthesis of aromatic coumarins and aromatic alpha-quinolones which were evaluated in vitro for their protective potentialities against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage on human liver cell death, i.e., human hepatoma HepG2 cell line and human hepatocytes in primary culture. We found that the presence of a ... Read more >>

Eur J Med Chem (European journal of medicinal chemistry)
[2004, 39(11):931-937]

Cited: 7 times

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Reanalysis of the testosterone displacement from its HSA binding site by DHEA using competitive Langmuir isotherm.

Claire André, Alain Xicluna, Jean-François Robert, Mireille Thomassin, Yves-Claude Guillaume,

In a previous manuscript [C. Andre, A. Berthelot, J.F. Robert, M. Thomassin, Y.C. Guillaume, J. Pharm. Bio. Med., in press], the Mg(2+) effect on the testosterone displacement equilibrium from its human serum albumin (HSA) binding site by DHEA was investigated using a thermodynamic approach. In this paper, a novel concept ... Read more >>

Talanta (Talanta)
[2005, 65(3):814-818]

Cited: 6 times

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Concentration of sodium ion as the determining factor for the association of dansyl amino acids with the teicoplanin molecule in reversed-phase liquid chromatography.

Lhassane Ismaili, Tong Than Truong, Claire André, Mireille Thomassin, Jean-Louis Mozer, Jean-Francois Robert, Alain Xicluna, Bernard Refouvelet, Joelle Millet, Laurence Nicod, Yves-Claude Guillaume,

The mechanism of the binding of D,L dansyl amino acids to teicoplanin was investigated. Na+ was used as an indicator of the interactions between the solutes and teicoplanin. The number (n) of sodium ions, Na+, excluded from the solute-teicoplanin interface when analyte transfer occurred was determined. A thermodynamic study and ... Read more >>

J AOAC Int (Journal of AOAC International)
[2003, 86(2):222-228]

Cited: 2 times

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Method for preparing 13 5-triaminobenzene and hydrolyzing it into high-purity phloroglucinal


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Cited: 0 times

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Triazine-human serum albumin association: thermodynamic approach and sodium effect.

Lhassane Ismaili, Claire André, Laurence Nicod, Tong Thanh Truong, Joëlle Millet, Mireille Thomassin, Eric Cavalli, Jean Pierre Chaumont, Alain Xicluna, Yves Claude Guillaume,

Human serum albumin (HSA) serves as a carrier protein to transport triazine herbicides to molecular targets. In this paper, a theoretical treatment was developed to describe the HSA-triazine herbicides association. A determination of the association constant, K, as well as the degree of complexation n(c) (the percent of complex guest) ... Read more >>

J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (Journal of chromatography. B, Analytical technologies in the biomedical and life sciences)
[2002, 780(2):467-474]

Cited: 3 times

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Pharmacological profile of 6,12-dihydro-3-methoxy-1-benzopyrano[3,4-b] [1,4]benzothiazin-6-one, a novel human estrogen receptor agonist.

Yves Jacquot, Anny Cleeren, Ioanna Laios, Ma Yan, Athem Boulahdour, Laurent Bermont, Bernard Refouvelet, Gérard Adessi, Guy Leclercq, Alain Xicluna,

Pharmacological studies were carried out to characterize further the endocrinological profile and the binding mode to the estrogen receptor (ER) of 6,12-dihydro-3-methoxy-1-benzopyrano[3,4-b][1,4]benzothiazin-6-one (1). Binding experiments were conducted with highly purified recombinant human estrogen receptors hERa and beta. Potent estrogenic activity of compound 1 was assessed by testing its ability to ... Read more >>

Biol. Pharm. Bull. (Biological & pharmaceutical bulletin)
[2002, 25(3):335-341]

Cited: 7 times

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Separation in slalom chromatography: stretching and velocity dependence.

Yves Claude Guillaume, François Xavier Perrin, Christiane Guinchard, Laurence Nicod, Tong Thanh Truong, Alain Xicluna, Joelle Millet, Mireille Thomassint,

Slalom chromatography (SC) is used for the separation of large double-stranded DNA molecules. In this technique, the progression of the DNA fragments through the closed column packing follows the flow direction and is like a snake edging is way into long grass. A novel mathematical model is developed in this ... Read more >>

Anal. Chem. (Analytical chemistry)
[2002, 74(6):1217-1222]

Cited: 1 time

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