Full Text Journal Articles by
Author Aiying Li

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Establishment of recombineering genome editing system in Paraburkholderia megapolitana empowers activation of silent biosynthetic gene clusters.

Wentao Zheng, Xue Wang, Haibo Zhou, Youming Zhang, Aiying Li, Xiaoying Bian,

The Burkholderiales are an emerging source of bioactive natural products. Their genomes contain a large number of cryptic biosynthetic gene clusters (BGCs), indicating great potential for novel structures. However, the lack of genetic tools for the most of Burkholderiales strains restricts the mining of these cryptic BGCs. We previously discovered ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):397-405]

Cited: 0 times

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Recombineering for Genetic Engineering of Natural Product Biosynthetic Pathways.

Muhammad Nazeer Abbasi, Jun Fu, Xiaoying Bian, Hailong Wang, Youming Zhang, Aiying Li,

Microbial genomes encode many cryptic and uncharacterized biosynthetic gene clusters (BGCs). Exploiting this unexplored genetic wealth to discover microbial novel natural products (NPs) remains a challenging issue. We review homologous recombination (HR)-based recombineering, mediated by the recombinases RecE/RecT from Rac prophage and Redα/Redβ from lambda phage, which has developed into ... Read more >>

Trends Biotechnol. (Trends in biotechnology)
[2020, :]

Cited: 0 times

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Single-Stranded DNA-Binding Protein and Exogenous RecBCD Inhibitors Enhance Phage-Derived Homologous Recombination in Pseudomonas.

Jia Yin, Wentao Zheng, Yunsheng Gao, Chanjuan Jiang, Hongbo Shi, Xiaotong Diao, Shanshan Li, Hanna Chen, Hailong Wang, Ruijuan Li, Aiying Li, Liqiu Xia, Yulong Yin, A Francis Stewart, Youming Zhang, Jun Fu,

The limited efficiency of the available tools for genetic manipulation of Pseudomonas limits fundamental research and utilization of this genus. We explored the properties of a lambda Red-like operon (BAS) from Pseudomonas aeruginosa phage Ab31 and a Rac bacteriophage RecET-like operon (RecTEPsy) from Pseudomonas syringae pv. syringae B728a. Compared with ... Read more >>

iScience (iScience)
[2019, 14:1-14]

Cited: 0 times

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Enhanced Heterologous Spinosad Production from a 79-kb Synthetic Multioperon Assembly.

Chaoyi Song, Ji Luan, Qingwen Cui, Qiuyue Duan, Zhen Li, Yunsheng Gao, Ruijuan Li, Aiying Li, Yuemao Shen, Yuezhong Li, A Francis Stewart, Youming Zhang, Jun Fu, Hailong Wang,

Refactoring biosynthetic pathways for enhanced secondary metabolite production is a central challenge for synthetic biology. Here we applied advanced DNA assembly methods and a uniform overexpression logic using constitutive promoters to achieve efficient heterologous production of the complex insecticidal macrolide spinosad. We constructed a 79-kb artificial gene cluster in which ... Read more >>

ACS Synth Biol (ACS synthetic biology)
[2019, 8(1):137-147]

Cited: 1 time

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Expressing cytotoxic compounds in Escherichia coli Nissle 1917 for tumor-targeting therapy.

Ruijuan Li, Linda Helbig, Jun Fu, Xiaoying Bian, Jennifer Herrmann, Michael Baumann, A Francis Stewart, Rolf Müller, Aiying Li, Daniel Zips, Youming Zhang,

Abnormal blood vessels and hypoxic and necrotic regions are common features of solid tumors and related to the malignant phenotype and therapy resistance. Certain obligate or facultative anaerobic bacteria exhibit inherent ability to colonize and proliferate within solid tumors in vivo. Escherichia coli Nissle 1917 (EcN), a non-pathogenic probiotic in European ... Read more >>

Res. Microbiol. (Research in microbiology)
[2019, 170(2):74-79]

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Virtual Screening and Biomolecular Interactions of CviR-Based Quorum Sensing Inhibitors Against Chromobacterium violaceum.

Vinothkannan Ravichandran, Lin Zhong, Hailong Wang, Guangle Yu, Youming Zhang, Aiying Li,

The rise of bacterial multi drug resistance becomes a global threat to the mankind. Therefore it is essential to find out alternate strategies to fight against these "super bugs." Quorum sensing (QS) is a cell-to-cell communication mechanism by which many bacteria regulate their biofilm and virulence factors expression to execute ... Read more >>

Front Cell Infect Microbiol (Frontiers in cellular and infection microbiology)
[2018, 8:292]

Cited: 3 times

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Adjuvant chemotherapy for stage II colon cancer: who really needs it.

Yan Yang, Yang Yang, Hui Yang, Fen Wang, Huihui Wang, Qi Chen, Ying Liu, Aiying Li, Quanan Zhang,

Although there is evidence that failure to reach the baseline of 12-13 lymph nodes in resected specimens is related to poor prognosis of patients with stage II colon cancer, and may be a marker of adjuvant therapy, the use of these markers remains controversial. The objective of this study was ... Read more >>

Cancer Manag Res (Cancer management and research)
[2018, 10:2509-2520]

Cited: 0 times

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Heterologous Expression Guides Identification of the Biosynthetic Gene Cluster of Chuangxinmycin, an Indole Alkaloid Antibiotic.

Xiaokun Xu, Haibo Zhou, Yang Liu, Xiaotong Liu, Jun Fu, Aiying Li, Yue-Zhong Li, Yuemao Shen, Xiaoying Bian, Youming Zhang,

The indole alkaloid antibiotic chuangxinmycin, from Actinobacteria Actinoplanes tsinanensis, containing a unique thiopyrano[4,3,2- cd]indole scaffold, is a potent and selective inhibitor of bacterial tryptophanyl-tRNA synthetase. The chuangxinmycin biosynthetic gene cluster was identified by in silico analysis of the genome sequence, then verified by heterologous expression. Systemic gene inactivation and intermediate ... Read more >>

J. Nat. Prod. (Journal of natural products)
[2018, 81(4):1060-1064]

Cited: 2 times

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ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes.

Hailong Wang, Zhen Li, Ruonan Jia, Jia Yin, Aiying Li, Liqiu Xia, Yulong Yin, Rolf Müller, Jun Fu, A Francis Stewart, Youming Zhang,

The exponentially increasing volumes of DNA sequence data highlight the need for new DNA cloning methods to explore the new information. Here, we describe 'ExoCET' (Exonuclease Combined with RecET recombination) to directly clone any chosen region from bacterial and mammalian genomes with nucleotide precision into operational plasmids. ExoCET combines in ... Read more >>

Nucleic Acids Res. (Nucleic acids research)
[2018, 46(5):e28]

Cited: 6 times

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ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes.

Hailong Wang, Zhen Li, Ruonan Jia, Jia Yin, Aiying Li, Liqiu Xia, Yulong Yin, Rolf Müller, Jun Fu, A Francis Stewart, Youming Zhang,

Nucleic Acids Res. (Nucleic acids research)
[2018, 46(5):2697]

Cited: 1 time

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Heterologous Production and Yield Improvement of Epothilones in Burkholderiales Strain DSM 7029.

Xiaoying Bian, Biao Tang, Yucong Yu, Qiang Tu, Frank Gross, Hailong Wang, Aiying Li, Jun Fu, Yuemao Shen, Yue-Zhong Li, A Francis Stewart, Guoping Zhao, Xiaoming Ding, Rolf Müller, Youming Zhang,

The cloning of microbial natural product biosynthetic gene clusters and their heterologous expression in a suitable host have proven to be a feasible approach to improve the yield of valuable natural products and to begin mining cryptic natural products in microorganisms. Myxobacteria are a prolific source of novel bioactive natural ... Read more >>

ACS Chem. Biol. (ACS chemical biology)
[2017, 12(7):1805-1812]

Cited: 8 times

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Mangiferin protects mitochondrial function by preserving mitochondrial hexokinase-II in vessel endothelial cells.

Junna Song, Yi Li, Junmei Song, Fangjie Hou, Baolin Liu, Aiying Li,

Hexokinase-II (HK-II) confers protection against cell death and this study was designed to investigate the effect of mangiferin on the regulation of mitochondrial HK-II. In vessel endothelial cells, saturated fatty acid palmitate (PA) stimulation induced HK-II detachment from mitochondria due to cellular acidification. Mangiferin reduced lactate accumulation by improving pyruvate ... Read more >>

Biochim Biophys Acta Mol Basis Dis (Biochimica et biophysica acta. Molecular basis of disease)
[2017, 1863(7):1829-1839]

Cited: 5 times

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A Practical Guide to Recombineering in Photorhabdus and Xenorhabdus.

Jia Yin, Hailong Wang, Ruijuan Li, Vinothkannan Ravichandran, Xiaoying Bian, Aiying Li, Qiang Tu, A Francis Stewart, Jun Fu, Youming Zhang,

Fluent genetic manipulation of prokaryote genomes is still limited to only a few commonly used hosts. Ideally the advanced technologies available for cloning into recombinant Escherichia coli should also be applicable in other prokaryotes. In particular, 'recombineering' is mediated by the lambda Red operon that permits fluent and precise engineering ... Read more >>

Curr. Top. Microbiol. Immunol. (Current topics in microbiology and immunology)
[2017, 402:195-213]

Cited: 1 time

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RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression.

Hailong Wang, Zhen Li, Ruonan Jia, Yu Hou, Jia Yin, Xiaoying Bian, Aiying Li, Rolf Müller, A Francis Stewart, Jun Fu, Youming Zhang,

Full-length RecE and RecT from Rac prophage mediate highly efficient linear-linear homologous recombination that can be used to clone large DNA regions directly from genomic DNA into expression vectors, bypassing library construction and screening. Homologous recombination mediated by Redαβ from lambda phage has been widely used for recombinant DNA engineering. ... Read more >>

Nat Protoc (Nature protocols)
[2016, 11(7):1175-1190]

Cited: 18 times

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Acetylbritannilactone Modulates Vascular Endothelial Growth Factor Signaling and Regulates Angiogenesis in Endothelial Cells.

Jingshan Zhao, Honglin Niu, Aiying Li, Lei Nie,

The present study was conducted to determine the effects of 1-O-acetylbritannilactone (ABL), a compound extracted from Inula britannica L., on vascular endothelial growth factor (VEGF) signaling and angiogenesis in endothelial cells (ECs). We showed that ABL promotes VEGF-induced cell proliferation, growth, migration, and tube formation in cultured human ECs. Furthermore, ... Read more >>

PLoS ONE (PloS one)
[2016, 11(2):e0148968]

Cited: 0 times

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Expression, Crystallization and Preliminary X-ray Diffraction Analyses of Med-ORF10 in the Biosynthetic Pathway of an Antitumor Antibiotic Medermycin.

Yanli Liu, Shasha Liu, Tingting Yang, Xiaoxia Guo, Yali Jiang, Kashif Rafiq Zahid, Ke Liu, Jinlin Liu, Jihong Yang, Haobin Zhao, Yi Yang, Aiying Li, Chao Qi,

Medermycin, as a prominent member of benzoisochromanequinones, possesses strong antitumor activity and is biosynthesized under the control of a 29-ORF-containing biosynthetic gene cluster. Most of ORFs in this gene cluster have not been characterized, including a small protein encoding gene med-ORF10, proposed to play a regulatory role in biosynthesis of ... Read more >>

Protein J. (The protein journal)
[2015, 34(6):404-410]

Cited: 1 time

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Characterization of a novel DNA glycosylase from S. sahachiroi involved in the reduction and repair of azinomycin B induced DNA damage.

Shan Wang, Kai Liu, Le Xiao, LiYuan Yang, Hong Li, FeiXue Zhang, Lei Lei, ShengQing Li, Xu Feng, AiYing Li, Jing He,

Azinomycin B is a hybrid polyketide/nonribosomal peptide natural product and possesses antitumor activity by interacting covalently with duplex DNA and inducing interstrand crosslinks. In the biosynthetic study of azinomycin B, a gene (orf1) adjacent to the azinomycin B gene cluster was found to be essential for the survival of the ... Read more >>

Nucleic Acids Res. (Nucleic acids research)
[2016, 44(1):187-197]

Cited: 7 times

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Functional Characterization of a Ketoreductase-Encoding Gene med-ORF12 Involved in the Formation of a Stereospecific Pyran Ring during the Biosynthesis of an Antitumor Antibiotic Medermycin.

Qiang He, Le Li, Tingting Yang, Ruijuan Li, Aiying Li,

Medermycin, a polyketide antibiotic, possesses strong bioactivity against a variety of tumors through a novel mechanism and is structurally featured with a pyran ring containing two chiral centers (3S and 15R). By far the biosynthetic origin of such enantiomerical conformations still remains obscure. In the present study, we reported the ... Read more >>

PLoS ONE (PloS one)
[2015, 10(7):e0132431]

Cited: 3 times

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Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.

Jin Lü, Qiang He, Luyao Huang, Xiaofeng Cai, Wenwen Guo, Jing He, Lili Zhang, Aiying Li,

Medermycin and kalafungin, two antibacterial and antitumor antibiotics isolated from different streptomycetes, share an identical polyketide skeleton core. The present study reported the discovery of kalafungin in a medermycin-producing streptomycete strain for the first time. A mutant strain obtained through UV mutagenesis showed a 3-fold increase in the production of ... Read more >>

PLoS ONE (PloS one)
[2015, 10(2):e0117690]

Cited: 4 times

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Metabolomic profiling of human serum in lung cancer patients using liquid chromatography/hybrid quadrupole time-of-flight mass spectrometry and gas chromatography/mass spectrometry.

Yingrong Chen, Zhihong Ma, Aiying Li, Hongwei Li, Bin Wang, Jing Zhong, Lishan Min, Licheng Dai,

Lung cancer is one of the most common causes of death from cancer. Serum markers that enable diagnosis of the disease in the early stage have not been found.Serum samples were collected from 30 healthy volunteers and from 30 lung cancer patients preoperatively and postoperatively. Samples were subjected to metabolomic ... Read more >>

J. Cancer Res. Clin. Oncol. (Journal of cancer research and clinical oncology)
[2015, 141(4):705-718]

Cited: 16 times

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Identification and characterization of a new acid-stable endoglucanase from a metagenomic library.

La Xiang, Aiying Li, Chaoguang Tian, Yuling Zhou, Guimin Zhang, Yanhe Ma,

A new endoglucanase gene cel124 was cloned from a metagenomic library and expressed in Escherichiacoli. Catalytic triad analysis showed that the catalytic triad sites were different from the known endoglucanases. Cel124, a 34 kDa protein, exhibited a specific activity (29.08 U mg(-1)) toward 1% of sodium carboxymethyl cellulose and was ... Read more >>

Protein Expr. Purif. (Protein expression and purification)
[2014, 102:20-26]

Cited: 5 times

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Distribution, cleavage and lipidation of Atg8 fusion proteins in Spodoptera litura Sl-HP cells.

Xiaojuan Zhang, Hongjuan Lu, Hui Ai, Rong Peng, Yongbo Yang, Aiying Li, Huazhu Hong, Jianxin Peng, Kaiyu Liu,

Atg8 proteins fused with tags are commonly used to detect autophagy. The expression patterns of Lepidopteran insect Atg8 are relatively well documented. However, the influence of protein tags on characterization of Atg8 is still not very clear. Our results showed that endogenous Spodoptera litura Atg8 and HA tagged Atg8 driven ... Read more >>

PLoS ONE (PloS one)
[2014, 9(5):e96059]

Cited: 1 time

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Identification and characterization of a novel alkaline α‑amylase Amy703 belonging to a new clade from Bacillus pseudofirmus.

Zhenghui Lu, Chaoguang Tian, Aiying Li, Guimin Zhang, Yanhe Ma,

Alkaline α-amylases are of great interest in desizing processes and detergent industries. Here, an alkaline α-amylase gene amy703 from an alkaliphilic Bacillus pseudofirmus strain was cloned and sequenced. Its encoding product, Amy703, might represent a new clade of α-amylase family, because it shared only 35 % highest identity with all ... Read more >>

J. Ind. Microbiol. Biotechnol. (Journal of industrial microbiology & biotechnology)
[2014, 41(5):783-793]

Cited: 4 times

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Characterization of Atg8 in lepidopteran insect cells.

Zhongchao Gai, Xiaojuan Zhang, Mayira Islam, Xia Wang, Aiying Li, Yongbo Yang, Yi Li, Jianxin Peng, Huazhu Hong, Kaiyu Liu,

Yeast Atg8 and mammalian microtubule-associated protein light chain 3 (LC3) are landmark proteins essential for autophagy. Here the lepidopteran Atg8, a homolog of LC3, is characterized. Sequence analysis reveals that Atg8 proteins are highly conserved in lepidopteran species. The abundance of endogeous Atg8 and the ratios of Atg8 conjugation to ... Read more >>

Arch. Insect Biochem. Physiol. (Archives of insect biochemistry and physiology)
[2013, 84(2):57-77]

Cited: 10 times

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An extremophile Microbacterium strain and its protease production under alkaline conditions.

Jin Lü, Xiaodan Wu, Yali Jiang, Xiaofeng Cai, Luyao Huang, Yongbo Yang, Huili Wang, Aibing Zeng, Aiying Li,

Extremophiles are potential resources for alkaline protease production. In order to search for alkaline protease producers, we isolated and screened alkaliphilic microorganisms from alkaline saline environments. The microorganism HSL10 was identified as a member of the genus Microbacterium by morphological observation, Gram staining and sequence analysis of the 16S rRNA ... Read more >>

J. Basic Microbiol. (Journal of basic microbiology)
[2014, 54(5):378-385]

Cited: 2 times

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