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Arabidopsis thaliana growth is independently controlled by the SUMO E3 ligase SIZ1 and Hexokinase 1.

Pedro Humberto Castro, Nuno Verde, Herlander Azevedo,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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nu444 is a novel allele of pkc-1 in C. elegans.

Han Wang, Derek Sieburth,

MicroPubl Biol (microPublication biology)
[2017, 2017:]

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Cloning and Detection of Aptamer-Ribozyme Conjugations.

Ryan P Goguen, Anne Gatignol, Robert J Scarborough,

RNA aptamers can be used to target proteins or nucleic acids for therapeutic purposes and are candidates for RNA-mediated gene therapy. Like other small therapeutic RNAs, they can be expressed in cells from DNA templates that include a cellular promoter upstream of the RNA coding sequence. Secondary structures flanking aptamers ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2021, 2167:253-267]

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Conjugation as a Highly Sensitive Assay to Study Group II Intron Splicing In Vivo.

Félix LaRoche-Johnston, Caroline Monat, Benoit Cousineau,

Group II introns are noncoding sequences that interrupt genes, and that must be removed or spliced-out at the RNA level during gene expression. Following the transcription of interrupted genes, group II introns self-splice while concurrently ligating their flanking exons to generate mature mRNAs ready for translation. Ll.LtrB, the model group ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2021, 2167:3-11]

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C-to-U RNA Editing: From Computational Detection to Experimental Validation.

Taga Lerner, Mitchell Kluesner, Rafail Nikolaos Tasakis, Branden S Moriarity, F Nina Papavasiliou, Riccardo Pecori,

The AID/APOBEC family of enzymes are cytidine deaminases that act upon DNA and RNA. Among APOBECs, the best characterized family member to act on RNA is the enzyme APOBEC1. APOBEC1-mediated RNA editing plays a key role in lipid metabolism and in maintenance of brain homeostasis. Editing can be easily detected ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2021, 2181:51-67]

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Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154.

Rongyuan Zhang, Weijie Zhang, Baocai Xu, Chuan Lv, Jianquan Hou, Guangbo Zhang,

Renal carcinoma (RCC) is widely accepted as a malignant tumour of urinary system. Long intergenic non-coding RNA 1939 (LINC01939) is a novel lncRNA which was found to be down-regulated in RCC. Thus, we set out to explore the effect and regulation mechanism of LINC01939 in RCC. LINC01939 and miR-154 in ... Read more >>

Artif Cells Nanomed Biotechnol (Artificial cells, nanomedicine, and biotechnology)
[2020, 48(1):695-702]

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Contribution of NKT cells to the immune response and pathogenesis triggered by respiratory viruses.

Emma Rey-Jurado, Karen Bohmwald, Nicolás M S Gálvez, Daniela Becerra, Steven A Porcelli, Leandro J Carreño, Alexis M Kalergis,

Human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) cause acute respiratory tract infections in children worldwide. Natural killer T (NKT) cells are unconventional T lymphocytes, and their TCRs recognize glycolipids bound to the MHC-I-like molecule, CD1d. These cells modulate the inflammatory response in viral infections. Here, we evaluated the ... Read more >>

Virulence (Virulence)
[2020, 11(1):580-593]

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Altered levels of complement components associated with non-immediate drug hypersensitivity reactions.

Feng Wang, Liping Huang, Junfeng Yu, Dandan Zang, Liangping Ye, Qixing Zhu,

Nonimmediate drug hypersensitivity reactions (niDHRs) range from mild-type maculopapular exanthema (MPE) to severe type Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) with unentirely clarified pathogenesis. This study sought to explore whether complement components participated in niDHRs. The participants comprised of three groups as follows: MPE (n = 65), SJS/TEN (n = 13, contains ... Read more >>

J Immunotoxicol (Journal of immunotoxicology)
[2020, 17(1):1-9]

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B cell intrinsic expression of IFNλ receptor suppresses the acute humoral immune response to experimental blood-stage malaria.

William O Hahn, Marion Pepper, W Conrad Liles,

Antibodies play a critical protective role in the host response to blood-stage malaria infection. The role of cytokines in shaping the antibody response to blood-stage malaria is unclear. Interferon lambda (IFNλ), a type III interferon, is a cytokine produced early during blood-stage malaria infection that has an unknown physiological role ... Read more >>

Virulence (Virulence)
[2020, 11(1):594-606]

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Delayed specific IgM antibody responses observed among COVID-19 patients with severe progression.

Liang Shen, Chunhua Wang, Jianzhong Zhao, Xiaoyong Tang, Ying Shen, Mingqing Lu, Zhe Ding, Canping Huang, Ji Zhang, Shichao Li, Jiaming Lan, Gary Wong, Yufang Zhu,

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly worldwide since it was confirmed as the causative agent of COVID-19. Molecular diagnosis of the disease is typically performed via nucleic acid-based detection of the virus from swabs, sputum or bronchoalveolar lavage fluid (BALF). However, the positive rate from the ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2020, 9(1):1096-1101]

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Shuxuening injection, derived from Ginkgo biloba leaf, induced pseudo-allergic reactions through hyperactivation of mTOR.

Lianmei Wang, Jingzhuo Tian, Suyan Liu, Yanyan Zhang, Jing Liu, Yan Yi, Chunying Li, Yong Zhao, Yushi Zhang, Jiayin Han, Chen Pan, Guiqin Li, Zhong Xian, Aihua Liang,

Context: Shuxuening injection (SXNI), derived from the leaf of Ginkgo biloba L. (Ginkgoaceae), is widely used to treat cardio-cerebral vascular system related disease due to the efficacy of dilating the blood vessels and improving the function of microcirculation. Nevertheless, SXNI induces immediate hypersensitivity reactions in clinics and the molecular mechanisms ... Read more >>

Pharm Biol (Pharmaceutical biology)
[2020, 58(1):581-589]

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Cross-validation of the RT-QuIC assay for the antemortem detection of chronic wasting disease in elk.

N J Haley, R Donner, D M Henderson, J Tennant, E A Hoover, M Manca, B Caughey, N Kondru, S Manne, A Kanthasamay, S Hannaoui, S C Chang, S Gilch, S Smiley, G Mitchell, A D Lehmkuhl, B V Thomsen,

Chronic wasting disease is a progressively fatal, horizontally transmissible prion disease affecting several members of the cervid species. Conventional diagnosis relies on ELISA or IHC evaluation using tissues collected post-mortem; however, recent research has focused on newly developed amplification techniques using samples collected antemortem. The present study sought to cross-validate ... Read more >>

Prion (Prion)
[2020, 14(1):47-55]

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Potential classification of chemical immunologic response based on gene expression profiles.

Stacey E Anderson, Rachel Baur, Michael Kashon, Ewa Lukomska, Lisa Weatherly, Hillary L Shane,

Occupational immune diseases are a serious public health burden and are often a result of exposure to low molecular weight (LMW) chemicals. The complete immunological mechanisms driving these responses are not fully understood which has made the classification of chemical allergens difficult. Antimicrobials are a large group of immunologically-diverse LMW ... Read more >>

J Immunotoxicol (Journal of immunotoxicology)
[2020, 17(1):122-134]

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Activation of aryl hydrocarbon receptor (AhR) in mesenchymal stem cells modulates macrophage polarization in asthma.

Zhuang Cui, Yuan Feng, Danqing Li, Taoping Li, Peisong Gao, Ting Xu,

Macrophage polarization has been demonstrated to exert a vital role on asthma pathogenesis. Mesenchymal stem cells (MSC) have the capacity to modulate macrophage differentiation from a pro-inflammatory M1 phenotype toward an anti-inflammatory M2 phenotype. However, the impact of MSC-macrophage interactions on asthma development and underlying mechanisms responsible for this interaction ... Read more >>

J Immunotoxicol (Journal of immunotoxicology)
[2020, 17(1):21-30]

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Recent advances on the role of long non-coding RNAs in Alzheimer's disease.

Kyle Doxtater, Manish K Tripathi, Mohammad Moshahid Khan,

Neural Regen Res (Neural regeneration research)
[2020, 15(12):2253-2254]

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LncRNA SNHG4 regulates miR-10a/PTEN to inhibit the proliferation of acute myeloid leukemia cells.

Zhongtao Yuan, Wei Wang,

ABSTRACTObjectives: Long non-coding RNA (lncRNA) small nucleolar RNA host gene 4 (SNHG4) is a characterized oncogenic lncRNA in osteosarcoma. The analysis of the TCGA dataset suggested the downregulation of SNHG4 in acute myeloid leukemia (AML), indicating its possible involvement in this disease. Therefore, this study was performed to analyze the ... Read more >>

Hematology (Hematology (Amsterdam, Netherlands))
[2020, 25(1):160-164]

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Implementation and evaluation of a self-directed learning activity for first-year medical students.

Molly Hill, Megan Peters, Michelle Salvaggio, Jay Vinnedge, Alix Darden,

Background: It is critical that medical students develop self-directed, life-long learning skills to navigate medical school successfully and to become competent healthcare professionals. Moreover, the Liaison Committee on Medical Education (LCME), the USA medical school accrediting body, requires activities designed to help students develop self-directed learning (SDL) skills in the ... Read more >>

Med Educ Online (Medical education online)
[2020, 25(1):1717780]

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Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors.

Huan Han, Qingfeng Ma, Cong Li, Rui Liu, Li Zhao, Wei Wang, Pingan Zhang, Xinghui Liu, Guosheng Gao, Fang Liu, Yingan Jiang, Xiaoming Cheng, Chengliang Zhu, Yuchen Xia,

Since the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China, it has rapidly spread across many other countries. While the majority of patients were considered mild, critically ill patients involving respiratory failure and multiple organ dysfunction syndrome are not uncommon, which could result death. We hypothesized that cytokine storm ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2020, 9(1):1123-1130]

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Prenatal arsenic exposure interferes in postnatal immunocompetence despite an absence of ongoing arsenic exposure.

Mainak Chakraborty, Moumita Bhaumik,

Arsenic (As) readily crosses the placenta and exposure of the fetus may cause adverse consequences later in life, including immunomodulation. In the current study, the question was asked how the immune repertoire might respond in postnatal life when there is no further As exposure. Here, pregnant mice (Balb/c [H-2d]) were ... Read more >>

J Immunotoxicol (Journal of immunotoxicology)
[2020, 17(1):135-143]

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Organic dust-induced lung injury and repair: Bi-directional regulation by TNFα and IL-10.

T A Wyatt, M Nemecek, D Chandra, J M DeVasure, A J Nelson, D J Romberger, J A Poole,

Exposure to organic dust increases chronic airway inflammatory disorders. Effective treatment strategies are lacking. It has been reported that hog barn dust extracts (HDE) induce TNFα through protein kinase C (PKC) activation and that lung inflammation is enhanced in scavenger receptor A (SRA/CD204) knockout (KO) mice following HDE. Because interleukin ... Read more >>

J Immunotoxicol (Journal of immunotoxicology)
[2020, 17(1):153-162]

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Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes.

Huiqiang Wu, Jing Wang, Qing Zhao, Yanjie Ding, Bingyi Zhang, Lingli Kong,

Rheumatoid arthritis (RA) is a chronic joint inflammatory disease that is closely associated with dysregulation of fibroblast-like synoviocytes (FLSs). Protocatechuic acid (PCA), a phenolic compound of anthocyanins, has been proven to possess anti-inflammatory activity. However, the role of PCA in RA has not been investigated. In the present study, we ... Read more >>

Artif Cells Nanomed Biotechnol (Artificial cells, nanomedicine, and biotechnology)
[2020, 48(1):969-976]

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The interaction between flagellin and the glycosphingolipid Gb3 on host cells contributes to Bacillus cereus acute infection.

Song Gao, Chengpei Ni, Wenhua Huang, Huaijie Hao, Hua Jiang, Qingyu Lv, Yuling Zheng, Peng Liu, Decong Kong, Yongqiang Jiang,

BACILLUS CEREUS:is an opportunistic pathogen that can cause emetic or diarrheal foodborne illness. Previous studies have identified multiple pathogenic B. cereus strains and characterized a variety of virulence factors. Here, we demonstrate that the virulence and lethality of B. cereus for mammalian cells and host animals involve the interaction of ... Read more >>

Virulence (Virulence)
[2020, 11(1):769-780]

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Colostrum fails to prevent bovine/camelid neonatal neutrophil damage from AFB1.

Sajad Najafi, Gholamreza Mohammadi, Mehrdad Mohri, Saman Hosseinkhani, Jalil Mehrzad,

Exposure to environmental toxicants that affect the immune system and overall health of many mammals is mostly unavoidable. One of the more common substances is the mycotoxins, especially carcinogenic aflatoxin (AF)B1 which also causes immune suppression/dysregulation in exposed hosts. The present study analyzed the effects of naturally occurring levels of ... Read more >>

J Immunotoxicol (Journal of immunotoxicology)
[2020, 17(1):43-50]

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Preclinical evaluation of antigen-specific nanotherapy based on phosphatidylserine-liposomes for type 1 diabetes.

Adrian Villalba, Silvia Rodriguez-Fernandez, Rosa-Maria Ampudia, Mary Cano-Sarabia, David Perna-Barrull, Cesc Bertran-Cobo, Clara Ehrenberg, Daniel Maspoch, Marta Vives-Pi,

Type 1 diabetes (T1D) is an autoimmune disease caused by the destruction of insulin-producing cells. Due to the ability of apoptotic cells clearance to induce tolerance, we previously generated liposomes rich in phophatidylserine (PS) -a feature of apoptotic cells- loaded with insulin peptides to mimic apoptotic beta-cells. PS-liposomes arrested autoimmunity ... Read more >>

Artif Cells Nanomed Biotechnol (Artificial cells, nanomedicine, and biotechnology)
[2020, 48(1):77-83]

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BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin.

Sonam Verma, Amandeep Kaur Kang, Rahul Pal, Satish Kumar Gupta,

The mechanism by which interferon-gamma (IFN-γ) downregulates trophoblast invasion needs further investigation. Treatment of HTR-8/SVneo cells with IFN-γ led to a decrease in their invasion concomitant with an increased expression of BST2. Silencing of BST2 by siRNA showed a significant increase in their invasion and spreading after treatment with IFN-γ ... Read more >>

Cell Adh Migr (Cell adhesion & migration)
[2020, 14(1):24-41]

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