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Modeling human glucose-6-phosphate dehydrogenase mutations using C. elegans GSPD-1.

Luiza N Loges, Katherine M Walstrom,

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked, recessive condition that causes intermittent jaundice or hemolytic anemia because of low NADPH levels in red blood cells. We performed steady-state enzyme kinetics with the recombinant <i>C. elegans</i> ortholog of human G6PD, GSPD-1, and two mutants containing amino acid changes found in human ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Over-expression of the constitutive AMPylase FIC-1(E274G) does not deplete cellular ATP pools in C. elegans.

Margaret Champion, Matthias C Truttmann,

Protein AMPylation has emerged as a posttranslational protein modification regulating cellular proteostasis. AMPylation is conferred by Fic AMPylases, which catalyze the covalent attachment of AMP to target proteins at the expense of ATP. Over-expression of constitutive-active Fic AMPylases is toxic. Here, we test the hypothesis that excessive Fic AMPylase activity ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis.

Gabriele Colozza,

Vertebrate embryonic development is regulated by a few families of extracellular signaling molecules. <i>Xenopus laevis</i> embryos offer an excellent system to study the cell-cell communication signals that govern embryonic patterning. In the frog embryos, Wnt/β-catenin plays a pivotal role in regulating embryonic axis development, and modulation of the Wnt pathway ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Expression of an endosome-excluded Cd63 prevents axis elongation in Xenopus.

Jennifer Kreis, Ramona Bonß, Kerstin Feistel, Philipp Vick,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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The tetraspanin Cd63 is required for eye morphogenesis in Xenopus.

Jennifer Kreis, Ramona Bonß, Philipp Vick,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Phenotypic modulation of pentylenetetrazole-induced convulsive behaviors in C. elegans carrying a mutation associated with Alzheimer's disease.

Madeline A Vaji, Guy A Caldwell, Kim A Caldwell,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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LOC496300 is expressed in the endoderm of developing Xenopus laevis embryos.

Maria Stewart, Kelsey Donahue, Elizabeth Wilke, Emily Shifley,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

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M1-type microglia can induce astrocytes to deposit chondroitin sulfate proteoglycan after spinal cord injury.

Shui-Sheng Yu, Zi-Yu Li, Xin-Zhong Xu, Fei Yao, Yang Luo, Yan-Chang Liu, Li Cheng, Mei-Ge Zheng, Jue-Hua Jing,

After spinal cord injury (SCI), astrocytes gradually migrate to and surround the lesion, depositing chondroitin sulfate proteoglycan-rich extracellular matrix and forming astrocytic scar, which limits the spread of inflammation but hinders axon regeneration. Meanwhile, microglia gradually accumulate at the lesion border to form microglial scar and can polarize to generate ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(5):1072-1079]

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Neutrophil-to-lymphocyte ratio in sporadic amyotrophic lateral sclerosis.

Qian-Qian Wei, Yan-Bing Hou, Ling-Yu Zhang, Ru-Wei Ou, Bei Cao, Yong-Ping Chen, Hui-Fang Shang,

The neutrophil-to-lymphocyte ratio (NLR) is considered a robust prognostic biomarker for predicting patient survival outcomes in many diseases. However, it remains unclear whether it can be used as a biomarker for amyotrophic lateral sclerosis (ALS). To correlate NLR with disease progression and survival in sporadic ALS, 1030 patients with ALS ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(4):875-880]

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Pre-clinical study of human umbilical cord mesenchymal stem cell transplantation for the treatment of traumatic brain injury: safety evaluation from immunogenic and oncogenic perspectives.

Gang Wang, Hua-Ling Wu, Yue-Ping Liu, De-Qi Yan, Zi-Lin Yuan, Li Chen, Qian Yang, Yu-Song Gao, Bo Diao,

Stem cell therapy is a promising strategy for the treatment of traumatic brain injury (TBI). However, animal experiments are needed to evaluate safety; in particular, to examine the immunogenicity and tumorigenicity of human umbilical cord mesenchymal stem cells (huMSCs) before clinical application. In this study, huMSCs were harvested from human ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(2):354-361]

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Lesion-induced changes of brevican expression in the perineuronal net of the superior vestibular nucleus.

Agnes Magyar, Eva Racz, Clara Matesz, Ervin Wolf, Peter Kiss, Botond Gaal,

Damage to the vestibular sense organs evokes static and dynamic deficits in the eye movements, posture and vegetative functions. After a shorter or longer period of time, the vestibular function is partially or completely restored via a series of processes such as modification in the efficacy of synaptic inputs. As ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(3):649-654]

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Angiotensin-(1-7) reduces α-synuclein aggregation by enhancing autophagic activity in Parkinson's disease.

Qing Gao, Rui Chen, Liang Wu, Qing Huang, Xi-Xi Wang, You-Yong Tian, Ying-Dong Zhang,

Abnormal accumulation of α-synuclein contributes to the formation of Lewy bodies in the substantia nigra, which is considered the typical pathological hallmark of Parkinson's disease. Recent research indicates that angiotensin-(1-7) plays a crucial role in several neurodegenerative disorders, including Parkinson's disease, but the underlying mechanisms remain elusive. In this study, ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(5):1138-1145]

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Low serum albumin level deteriorates prognosis in azacitidine-treated myelodysplastic syndromes patients - results of the PALG study 'PolAZA'.

Krzysztof Mądry, Karol Lis, Andrzej Tukiendorf, Paweł Szwedyk, Katarzyna Kapelko-Słowik, Edyta Subocz, Aleksandra Gołos, Wioletta Makowska, Anna Masternak, Anna Kopińska, Magdalena Czemerska, Sara Zawadzka-Leska, Patrycja Rusicka, Joanna Drozd-Sokołowska, Elżbieta Wiater, Jadwiga Hołojda, Bartłomiej Pogłódek, Piotr Centkowski, Anna Waszczuk-Gajda, Rafał Machowicz, Janusz Hałka, Tomasz Czerw, Grzegorz Basak, Jadwiga Dwilewicz-Trojaczek,

<h4>Background</h4>Azacitidine (AZA) is the standard of care for higher-risk myelodysplastic syndrome (HR-MDS) patients ineligible for intensive therapy. Clinical outcome discrepancies reported in clinical trials and real-life settings stimulate the search for new prognostic factors.<h4>Methods</h4>We retrospectively evaluated 315 MDS, 20-30% blast acute myeloid leukemia (AML) and chronic myelomonocytic leukemia (CMML) patients ... Read more >>

Hematology (Hematology (Amsterdam, Netherlands))
[2021, 26(1):556-564]

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Peritoneal transport status and first episode of peritonitis: a large cohort study.

Jing Hu, Hao Zhang, Bin Yi,

<h4>Background</h4>Peritonitis is one of the most serious complications of peritoneal dialysis (PD). This study aimed to explore the relationship between peritoneal transport status and the first episode of peritonitis, as well as the prognosis of patients undergoing continuous ambulatory peritoneal dialysis (CAPD).<h4>Method</h4>A retrospective cohort study was conducted, analyzing data of ... Read more >>

Ren Fail (Renal failure)
[2021, 43(1):1094-1103]

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Effects of selenium-containing compounds on Cu2+/Zn2+-induced neuronal cell death and potential application as therapeutic agents for neurological diseases.

Ken-Ichiro Tanaka, Mikako Shimoda, Masahiro Kawahara,

Neural Regen Res (Neural regeneration research)
[2022, 17(2):311-312]

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AGEs/RAGE blockade downregulates Endothenin-1 (ET-1), mitigating Human Umbilical Vein Endothelial Cells (HUVEC) injury in deep vein thrombosis (DVT).

Yunxin Zhang, Jianlong Liu, Wei Jia, Xuan Tian, Peng Jiang, Zhiyuan Cheng, Jinyong Li,

This study is aimed at identifying the roles of AGE/RAGE and ET-1 in deep vein thrombosis (DVT). Advanced glycation end products (AGEs) in glycated human serum albumin (M-HSA) were detected by ELISA. The viability of HUVECs was examined by CCK-8 assay. Flow cytometry was performed to detect cell apoptosis, followed ... Read more >>

Bioengineered (Bioengineered)
[2021, 12(1):1360-1368]

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Predialysis predictors for identifying patients requiring dialysis at a higher glomerular filtration rate.

Junseok Jeon, Hye Ryoun Jang, Wooseong Huh, Yoon-Goo Kim, Dae Joong Kim, Jung Eun Lee,

<h4>Background</h4>Current evidence suggests that the initiation of maintenance hemodialysis should not be based on a specific glomerular filtration rate (GFR) but on symptoms or signs attributable to kidney disease. However, it is difficult to predict the time point at which overt uremic syndrome develops in individuals. The estimated GFR is ... Read more >>

Ren Fail (Renal failure)
[2021, 43(1):1087-1093]

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Bovine serum albumin as a resuscitation promoting factor for viable but non-culturable Mycobacterium tuberculosis via the activation of protein kinase A-dependent cellular processes

Yuta Morishige, Yoshiro Murase, Kinuyo Chikamatsu, Akio Aono, Yuriko Igarashi, Yoshiko Shimomura, Makiko Hosoya, Keisuke Kamada, Hiroyuki Yamada, Akiko Takaki, Satoshi Mitarai,

<h4>Objective</h4> Mycobacterium tuberculosis (Mtb) H37Ra strain has been reported to rapidly enter the viable but non-culturable (VBNC) state following treatment with an NADH oxidase inhibitor (diphenyleneiodonium [DPI]) and to be resuscitated by fetal bovine serum (FBS). However, the mechanism underlying FBS-induced resuscitation is currently unclear. We tried to reveal the ... Read more >>

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Effect of clinical factors on trajectory of functional performance in patients undergoing hemodialysis.

Jin-Bor Chen, Lung-Chih Li, Wen-Chin Lee, Sin-Hua Moi, Cheng-Hong Yang,

<h4>Purpose</h4>This study aimed to investigate the association between clinical factors and temporary changes in functional performance in patients undergoing hemodialysis.<h4>Methods</h4>This was a retrospective, longitudinal observational study conducted from 2015 to 2017. Eight-two patients undergoing hemodialysis in the outpatient clinic were enrolled. Functional performance was measured using the Karnofsky Performance Status ... Read more >>

Ren Fail (Renal failure)
[2021, 43(1):90-96]

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Tacrolimus versus cyclophosphamide for patients with idiopathic membranous nephropathy and treated with steroids: a systematic review and meta-analysis of randomized controlled trials.

Haiting Huang, Zhao Liang, Xintong Zheng, Qin Qing, Xiuri Du, Zhiming Tang, Meili Wei, Chen Wang, Qiuhong Zhong, Xu Lin,

<h4>Background</h4>The therapeutic effects of tacrolimus (TAC) versus cyclophosphamide (CTX) were not fully illustrated for patients with idiopathic membranous nephropathy (IMN).<h4>Methods</h4>The PubMed, EmBase, Cochrane library, and CNKI were systematically searched throughout March 2020 for randomized controlled trials evaluating the therapeutic effects of TAC versus CTX for IMN patients treated with steroids. ... Read more >>

Ren Fail (Renal failure)
[2021, 43(1):840-850]

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Risk factors for calciphylaxis in Chinese hemodialysis patients: a matched case-control study.

Yuqiu Liu, Xiaoliang Zhang, Xiaotong Xie, Xin Yang, Hong Liu, Rining Tang, Bicheng Liu,

<h4>Introduction</h4>Calciphylaxis is a rare but potentially fatal disease commonly occurred in dialysis patients. Despite some previous studies on risk factors for calciphylaxis, there is still a lack of data from Chinese population.<h4>Methods</h4>The retrospective matched case-control study about calciphylaxis was performed in Zhongda Hospital affiliated to Southeast University. The case group ... Read more >>

Ren Fail (Renal failure)
[2021, 43(1):406-416]

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Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury.

Min Cai, Jian Shao, Bryant Yung, Yi Wang, Nan-Nan Gao, Xi Xu, Huan-Huan Zhang, Yu-Mei Feng, Deng-Bing Yao,

Wallerian degeneration is a complex biological process that occurs after nerve injury, and involves nerve degeneration and regeneration. Schwann cells play a crucial role in the cellular and molecular events of Wallerian degeneration of the peripheral nervous system. However, Wallerian degeneration regulating nerve injury and repair remains largely unknown, especially ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(4):845-853]

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The Infusion of Human Serum Albumin had no Benefit on Acute Pancreatitis Therapy: An Analysis of Patients in Two Observational Cohorts

Yifei Ma, Tianao Yan, Fengshuo Xu, Jiachun Ding, Bao Yang, Qingyong Ma, Zheng Wu, Jun Lyu, Zheng Wang,

<h4>Objective: </h4> Human serum albumin (HSA) infusion is a common administration in acute pancreatitis patients in the Intensive Care Unit (ICU), but its actual association with patients' outcomes has not been confirmed. The study was aimed to determine whether the in-hospital prognosis of ICU patients with acute pancreatitis could benefit ... Read more >>

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Platelet-to-albumin ratio: a risk factor associated with technique failure and mortality in peritoneal dialysis patients.

Yuqi Yang, Jing Yuan, Lu Liu, Shuwen Qie, Li Yang, Zha Yan,

<h4>Background</h4>Peritoneal dialysis (PD) patients have a high incidence of poor clinical outcomes, which is related to the inflammatory and nutritional status of this population. Platelet-to-albumin ratio (PAR), recently identified as a useful biomarker to monitor inflammation and nutrition, can predict a poor prognosis in various diseases. The aim of this ... Read more >>

Ren Fail (Renal failure)
[2021, 43(1):1359-1367]

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Serum potassium, albumin and vitamin B12 as potential oxidative stress markers of fungal peritonitis.

Lingling Liu, Kehang Xie, Mengmeng Yin, Xiaoqiu Chen, Binhuan Chen, Jianting Ke, Cheng Wang,

Biomarkers of oxidative stress (OS) have been poorly explored in fungal peritonitis (FP). Potassium is a regulator of pro-oxidants and antioxidants. Albumin and vitamin B12 (B12) are vital antioxidant agents in the circulatory system. This study aimed to investigate the antioxidative role of serum potassium, albumin and B12 in FP. ... Read more >>

Ann Med (Annals of medicine)
[2021, 53(1):2132-2141]

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