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The odd-1(tm848) mutation has no significant effect on brood size in Caenorhabditis elegans.

Gabriella M Scoca, Amy C Groth,

The genome of <i>Caenorhabditis elegans</i> contains two genes of the odd-skipped transcription factor family, <i>odd-1</i> and <i>odd-2.</i> In <i>C. elegans, odd-1</i> is expressed in the intestine. A deletion mutant (<i>tm848)</i> that removes most of the <i>odd-1</i> gene, including all three zinc fingers, has no significant effect on brood size when ... Read more >>

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[2021, 2021:]

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Reducing the expression of the Numb adaptor protein in neurons increases the searching behavior of Drosophila larvae.

Andrew Galbraith, Samuel Leone, Katherine Stuart, Josie Emery, M Katie Renkemeyer, Nicholas Pritchett, Lauren Galbraith, Haley Stuckmeyer, Brett Berke,

<i>Drosophila</i> larval crawling is easily-observable and relatively stereotyped. Crawling consists of linear locomotion interrupted by periods when the larvae pause, swing their heads, and change direction (a 'search'). Here we identify Numb, a peripheral membrane adaptor protein, as an important regulator of searching behavior. When Numb RNAi transgenes were expressed ... Read more >>

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[2021, 2021:]

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Effects of blunt force injuries in third-instar Drosophila larvae persist through metamorphosis and reduce adult lifespan.

Jorgo Lika, Rebeccah J Katzenberger, Barry Ganetzky, David A Wassarman,

Blunt force injuries are a significant cause of disability and death worldwide. Here, we describe a <i>Drosophila melanogaster</i> model of blunt force injury that can be used to investigate cellular and molecular mechanisms that underlie the short-term and long-term effects of injuries sustained at a juvenile stage of development. Injuries ... Read more >>

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[2021, 2021:]

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Swimming exercise reduces native ⍺-synuclein protein species in a transgenic C. elegans model of Parkinson's disease.

Minna Y Schmidt, Manish Chamoli, Gordon J Lithgow, Julie K Andersen,

Exercise has been historically recommended to prevent many disease conditions. Intense exercise in particular, has been shown to be beneficial for Parkinson's disease (PD) - stopping and even reversing symptoms in some patients. Recent research in mammalian animal models of Parkinson's have shown that exercise affects ⍺-synuclein aggregate species, considered ... Read more >>

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[2021, 2021:]

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Over-expression of the constitutive AMPylase FIC-1(E274G) does not deplete cellular ATP pools in C. elegans.

Margaret Champion, Matthias C Truttmann,

Protein AMPylation has emerged as a posttranslational protein modification regulating cellular proteostasis. AMPylation is conferred by Fic AMPylases, which catalyze the covalent attachment of AMP to target proteins at the expense of ATP. Over-expression of constitutive-active Fic AMPylases is toxic. Here, we test the hypothesis that excessive Fic AMPylase activity ... Read more >>

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[2021, 2021:]

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Acute exposure of Nicotine during Drosophila puncture injury activates an epidermal wound response reaction.

Chrissy Cherenfant, Michelle T Juarez,

Epidermal wound reaction after injury can be visualized in <i>Drosophila melanogaster</i> embryos by transgenic fluorescent wound reporters. A "local" reaction is limited to the epidermal cells surrounding a wound site occurs in <i>wildtype</i> embryos. A "global" reaction extends beyond the wound site to all epidermal cells occurs in immune response ... Read more >>

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[2021, 2021:]

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The MLK-1/SCD-4 Mixed Lineage Kinase/MAP3K functions to promote dauer formation upstream of DAF-2/InsR.

Neal R Rasmussen, Harold E Smith, David J Reiner,

The <i>C. elegans</i> dauer is an alternative third stage larva induced by dense population and adverse environmental conditions. Genes whose mutants caused dauer formation constitutive (Daf-c) and dauer formation defective (Daf-d) phenotypes were ordered via epistasis into a signaling network, with upstream DAF-7/TGF-beta and DAF-11/receptor guanylyl cyclase defining sensory branches ... Read more >>

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[2021, 2021:]

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Novel allele elh of the UBP14 gene affects plant organ size via cell expansion in Arabidopsis thaliana.

Rakesh David, Pei Qin Ng, Lisa M Smith, Iain R Searle,

Plant organ size control is an essential process of plant growth and development. The regulation of plant organ size involves a complicated network of genetic, molecular interactions, as well as the interplay of environmental factors. Here, we report a temperature-sensitive hypocotyl elongation EMS-generated mutant, hereby referred to as <i>elongated hypocotyl ... Read more >>

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[2021, 2021:]

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Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag.

Christine K Rourke, Darline Murat, Tyler J Hansen, Aimee Jaramillo-Lambert,

To investigate the dynamic localization of Topoisomerase II in live <i>C. elegans</i> we have generated a C-terminally GFP-tagged version of TOP-2 at the endogenous locus. We found that TOP-2::GFP localizes in a similar pattern to the previously published TOP-2::3XFLAG strain and does not disrupt the meiotic chromosome segregation functions of ... Read more >>

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[2021, 2021:]

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Recombination and biased segregation of mitochondrial genomes during crossing and meiosis of different Schizosaccharomyces pombe strains.

Stephan Kamrad, María Rodríguez-López, Shoumit Dey, Mimoza Hoti, Henry Wallace, Markus Ralser, Jürg Bähler,

During meiosis, tethering of parental mitochondria to opposite cell poles inhibits the mixing of mitochondria with different genomes and ensures uniparental inheritance in thestandard laboratory strain of fission yeast. We here investigate mitochondrial inheritance in crosses between natural isolates using tetrad dissection and next-generation sequencing. We find that colonies grown ... Read more >>

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[2021, 2021:]

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Rapid degeneration of Drosophila olfactory neurons in Orco mutant maxillary palps.

Darya Task, Christopher J Potter,

<i>Drosophila melanogaster</i> vinegar flies have two olfactory organs: the antenna and maxillary palp. Olfactory neurons in these tissues respond to odorants via odorant receptors. Insect odorant receptors are heterotetramers of two proteins: an odorant binding OrX subunit and an Odorant Receptor Co-Receptor (Orco). Mutation of <i>Orco</i> disrupts odorant receptor formation, ... Read more >>

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[2021, 2021:]

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ifas-1 is upregulated by fungal infection in a GPA-12 and STA-2-independent manner in the Caenorhabditis elegans epidermis.

Shizue Omi, Xing Zhang, Nishant Thakur, Nathalie Pujol,

Skin infection with the fungus <i>Drechmeria coniospora</i> leads to a transcriptional response in the worm epidermis. This involves an increased expression of a group of antimicrobial peptide (AMP) genes including those in the <i>nlp-29</i> and <i>cnc-2</i> clusters. The major pathways leading to the expression of these AMP genes have been ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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N-terminally endogenous mKate2-tagged HIM-4 in Caenorhabditis elegans.

Shinji Ihara,

The gene <i>him-4</i> encodes the <i>C. elegans</i> homolog of hemicentin, an evolutionarily conserved extracellular matrix protein. Despite being an extracellular matrix protein, mutations in him-4 result in pleiotropic defects during development and demonstrate tissue fragility. While previous studies in <i>C. elegans</i> have confirmed the localization of either transgenic HIM-4::GFP or ... Read more >>

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[2021, 2021:]

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Transcriptional Response to a Dauer-Inducing Ascaroside Cocktail in Late L1 in C. elegans.

Sarah M Cohen, Jessica J Sun, Frank C Schroeder, Paul W Sternberg,

The dauer diapause stage in <i>C. elegans</i> is a non-feeding alternative to the L3 larval stage that is highly resistant to harsh environmental conditions. The decision to enter dauer is a two-step process. First, L1 larvae encounter adverse conditions such as lack of food or overcrowding and decide to enter ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Differential effects on neuromuscular physiology between Sod1 loss-of-function mutation and paraquat-induced oxidative stress in Drosophila.

Atsushi Ueda, Atulya Iyengar, Chun-Fang Wu,

Oxidative stress is thought to be a major contributor to aging processes. Here, we report differential effects on neurotransmission caused by loss-of-function mutations of <i>Superoxide dismutase 1</i> (<i>Sod1</i>) and by paraquat (PQ) feeding in <i>Drosophila</i>. We demonstrated alterations in <i>Sod1</i> mutants; the larval neuromuscular junction displayed supernumerary discharges and the ... Read more >>

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[2021, 2021:]

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Atg27p co-fractionates with clathrin-coated vesicles in budding yeast.

Verónica A Segarra, Anupam Sharma, Sandra K Lemmon,

Atg27p, a single-pass transmembrane protein that functions in autophagy, localizes to a variety of cellular compartments including the pre-autophagosomal structure, late Golgi, vacuolar membrane, as well as early and late endosomes. Its cytoplasmic C-terminus contains a tyrosine sorting motif that allows for its transport to the vacuolar membrane and an ... Read more >>

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[2021, 2021:]

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Similar responsiveness between C57BL/6N and C57BL/6J mouse substrains to superovulation.

Miyuki Shindo, Hideki Tsumura, Kenji Miyado, Woojin Kang, Natsuko Kawano, Tomoko Yoshida, Maki Fukami, Mami Miyado,

Superovulation is a method for the drug-induced release of multiple eggs and useful for <i>in vitro</i> fertilization. Thus, its high efficiency largely reduces the number of mice used per experiment. We compared the responsivity to superovulation between C57BL/6N (B6N) and C57BL/6J (B6J) substrains. The average number of ovulated eggs was ... Read more >>

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[2021, 2021:]

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Subcellular localization of the voltage-gated K+ channel EGL-36 , a member of the KV3 subfamily, in the ciliated sensory neurons in C. elegans.

Sebiha Cevik, Oktay I Kaplan,

Delineated as the first cellular organelle in 1675 by Antonie van Leeuwenhoek, cilia did not receive much attention until the 2000s, when it became apparent that cilia played a key role in the development of embryos, a variety of signaling pathways. Therefore, collective efforts by many scientists have led to ... Read more >>

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[2021, 2021:]

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Serum-free adapted Drosophila S2R+ line is amenable to RNA interference.

Arthur Luhur, Daniel Mariyappa, Kristin M Klueg, Stephen L Rogers, Andrew C Zelhof,

We have previously adapted a select number of <i>Drosophila</i> cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNAi against <i>Rho1</i> ... Read more >>

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[2021, 2021:]

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Mutations in the Physcomitrium patens gene encoding Aminodeoxychorismate Synthase confer auxotrophic phenotypes.

Michael J Prigge, Yingluo Wang, Mark Estelle,

To facilitate genetic mapping of developmental mutants of <i>Physcomitrium patens</i>, we produced a genetic marker that combines recessive auxotrophy with dominant positive selection. We first identified the gene affected by the <i>pabB4</i> auxotrophic mutation and then replaced it with a cassette that confers antibiotic resistance. This strain may be used ... Read more >>

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[2021, 2021:]

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The slo-1 BK potassium channel interacts genetically with pmr-1 secretory pathway calcium ATPase during C. elegans embryonic cell migration.

Michellie Thurman, Haonan Sun, Sam Kubica, Vida Praitis,

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[2021, 2021:]

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Repetitive mild traumatic brain injury causes synergistic effects on mortality.

Ashley M Willes, Tori R Krcmarik, Alexander E Daughtry, Douglas J Brusich,

Repetitive mild TBI (rmTBI) events are common in the U.S. However, rmTBI is challenging to study and this contributes to a poor understanding of mechanistic bases for disease following these injuries. We used fruit flies (<i>D. melanogaster</i>) and a modified version of the high-impact trauma (HIT) method of TBI to ... Read more >>

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[2021, 2021:]

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Genetic interactions in a C. elegans sod-1 ALS model: glutamatergic neuron degeneration.

Jennifer F Osborne, Katherine S Yanagi, Anne C Hart,

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[2021, 2021:]

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Mutating two putative phosphorylation sites on ZHP-3 does not affect its localization or function during meiotic chromosome segregation.

Anna E Russo, Christian R Nelson, Needhi Bhalla,

Meiotic chromosome segregation depends on crossover recombination to link homologous chromosomes together and promote accurate segregation in the first meiotic division. In <i>Caenorhabditis elegans</i>, a conserved RING finger protein, ZHP-3, is essential for meiotic recombination and localizes to sites of crossover formation. Whether ZHP-3 is regulated to promote recombination remains ... Read more >>

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[2021, 2021:]

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A histamine-gated channel is an efficient negative selection marker for C. elegans transgenesis.

Sonia El Mouridi, Sarah AlHarbi, Christian Frøkjær-Jensen,

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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