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Atg27p co-fractionates with clathrin-coated vesicles in budding yeast.

Verónica A Segarra, Anupam Sharma, Sandra K Lemmon,

Atg27p, a single-pass transmembrane protein that functions in autophagy, localizes to a variety of cellular compartments including the pre-autophagosomal structure, late Golgi, vacuolar membrane, as well as early and late endosomes. Its cytoplasmic C-terminus contains a tyrosine sorting motif that allows for its transport to the vacuolar membrane and an ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Similar responsiveness between C57BL/6N and C57BL/6J mouse substrains to superovulation.

Miyuki Shindo, Hideki Tsumura, Kenji Miyado, Woojin Kang, Natsuko Kawano, Tomoko Yoshida, Maki Fukami, Mami Miyado,

Superovulation is a method for the drug-induced release of multiple eggs and useful for <i>in vitro</i> fertilization. Thus, its high efficiency largely reduces the number of mice used per experiment. We compared the responsivity to superovulation between C57BL/6N (B6N) and C57BL/6J (B6J) substrains. The average number of ovulated eggs was ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Subcellular localization of the voltage-gated K+ channel EGL-36 , a member of the KV3 subfamily, in the ciliated sensory neurons in C. elegans.

Sebiha Cevik, Oktay I Kaplan,

Delineated as the first cellular organelle in 1675 by Antonie van Leeuwenhoek, cilia did not receive much attention until the 2000s, when it became apparent that cilia played a key role in the development of embryos, a variety of signaling pathways. Therefore, collective efforts by many scientists have led to ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Serum-free adapted Drosophila S2R+ line is amenable to RNA interference.

Arthur Luhur, Daniel Mariyappa, Kristin M Klueg, Stephen L Rogers, Andrew C Zelhof,

We have previously adapted a select number of <i>Drosophila</i> cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNAi against <i>Rho1</i> ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Mutations in the Physcomitrium patens gene encoding Aminodeoxychorismate Synthase confer auxotrophic phenotypes.

Michael J Prigge, Yingluo Wang, Mark Estelle,

To facilitate genetic mapping of developmental mutants of <i>Physcomitrium patens</i>, we produced a genetic marker that combines recessive auxotrophy with dominant positive selection. We first identified the gene affected by the <i>pabB4</i> auxotrophic mutation and then replaced it with a cassette that confers antibiotic resistance. This strain may be used ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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The slo-1 BK potassium channel interacts genetically with pmr-1 secretory pathway calcium ATPase during C. elegans embryonic cell migration.

Michellie Thurman, Haonan Sun, Sam Kubica, Vida Praitis,

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Genetic interactions in a C. elegans sod-1 ALS model: glutamatergic neuron degeneration.

Jennifer F Osborne, Katherine S Yanagi, Anne C Hart,

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[2021, 2021:]

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Repetitive mild traumatic brain injury causes synergistic effects on mortality.

Ashley M Willes, Tori R Krcmarik, Alexander E Daughtry, Douglas J Brusich,

Repetitive mild TBI (rmTBI) events are common in the U.S. However, rmTBI is challenging to study and this contributes to a poor understanding of mechanistic bases for disease following these injuries. We used fruit flies (<i>D. melanogaster</i>) and a modified version of the high-impact trauma (HIT) method of TBI to ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Mutating two putative phosphorylation sites on ZHP-3 does not affect its localization or function during meiotic chromosome segregation.

Anna E Russo, Christian R Nelson, Needhi Bhalla,

Meiotic chromosome segregation depends on crossover recombination to link homologous chromosomes together and promote accurate segregation in the first meiotic division. In <i>Caenorhabditis elegans</i>, a conserved RING finger protein, ZHP-3, is essential for meiotic recombination and localizes to sites of crossover formation. Whether ZHP-3 is regulated to promote recombination remains ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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A histamine-gated channel is an efficient negative selection marker for C. elegans transgenesis.

Sonia El Mouridi, Sarah AlHarbi, Christian Frøkjær-Jensen,

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Gene bashing of ceh-6 locus identifies genomic regions important for ceh-6 rectal cell expression and rescue of its mutant lethality.

Arnaud Ahier, Shashi Kumar Suman, Sophie Jarriault,

Strong loss-of-function or null mutants can sometimes lead to a penetrant early lethality, impairing the study of these genes' function. This is the case for the <i>ceh-6</i> null mutant, which exhibits 100% penetrant lethality. Here, we describe how we used gene bashing to identify distinct regulatory regions in the <i>ceh-6</i> ... Read more >>

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Worms avoid a cat sensed repellent.

Brianna Ramos, Gareth Harris,

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[2020, 2020:]

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Variation between nematodes in a multi-sensory behavioral assay.

Renae Ellis, Gareth Harris,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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A mutation that alters the 255th glutamate to lysine in RSKS-1/S6 kinase reliably extends the lifespan of C. elegans.

Jongsun Lee, Sieun S Kim, Seung-Jae V Lee,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Ovarian somatic Piwi regulates nurse cell proliferation and oocyte specification in Drosophila.

Lauren E Gonzalez, Gina Zhu, Haifan Lin,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Piwi in the stem cell niche regulates nurse cell number and oocyte specification.

Gina Zhu, Lauren E Gonzalez, Haifan Lin,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Caenorhabditis elegans larvae undergo early developmental arrest on a diet of Gram-positive bacterium Enterococcus faecalis.

Madhumanti Dasgupta, Nagagireesh Bojanala, Meghana Shashikanth, Varsha Singh,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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The tetraspanin Cd63 is required for eye morphogenesis in Xenopus.

Jennifer Kreis, Ramona Bonß, Philipp Vick,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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lin-41 controls dauer formation and morphology via lin-29 in C. elegans.

Allison R Cale, Xantha Karp,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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In silico identification of Drosophila melanogaster genes encoding RNA polymerase subunits.

Steven J Marygold, Nazif Alic, David S Gilmour, Savraj S Grewal,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Auxin does not affect a suite of morphological or behavioral phenotypes in two wild-type C. elegans strains.

Troy A McDiarmid, Lexis D Kepler, Catharine H Rankin,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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C. elegans CYLC-2 localizes to sperm.

Amber R Krauchunas, Michael Werner, Nicholas Britt, Dawn S Chen, Amy S Maddox, Andrew Singson,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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An updated pGREEN-based vector suite for cost-effective cloning in plant molecular biology.

Anna I Pratt, Jan Knoblauch, Hans-Henning Kunz,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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The cadmium-responsive gene, cdr-6, does not influence Caenorhabditis elegans responses to exogenous zinc.

Kathryn S Evans, Erik C Andersen,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Endogenous expression and localization of CAV-1::GFP in C. elegans.

Dillon E Sloan, Joshua N Bembenek,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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