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The effectiveness of EGFR knockdown by RNAi lines varies depending on the tissue.

PMID: 35903780 (view PubMed database entry)
DOI: 10.17912/micropub.biology.000612 (read at publisher's website )
PMCID: PMC9315407 (free full text version available)

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Mikaela Follmer, Brandy Shrawder, Kara Eckert, Briana Heinly, Pavithra Vivekanand,

In <i>Drosophila</i> , the Epidermal growth factor receptor (EGFR) signaling pathway is known to be critically involved in multiple stages of development. We induced a loss of function phenotype in the eyes, wings, and somatic follicle cells using four EGFR RNAi lines: HMS05003 and JF02283, which produce short hairpin RNAs, as well as JF01368 and KK100051, which produce long hairpin RNAs. Using these four lines, we completed a systematic comparison of the ability of short hairpin vs long hairpin RNAi lines to produce loss-of-function phenotypes in the above-mentioned tissues. Tissue specific knockdown was achieved by using Gal4 drivers specific to the three tissues being studied. In the eyes and wings, the KK100051 line induced the most severe phenotype, while the JF01368 line was the least severe, but in the somatic follicle cells, the KK100051 line was the least effective, while the JF01368 and JF02283 lines were comparable with respect to phenotypic severity. We conclude that there is significant tissue specific variability exhibited by the different RNAi lines.

MicroPubl Biol (microPublication biology)
[2022, 2022:]

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