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Mycobacterium senriense sp. nov., a slowly growing, non-scotochromogenic species, isolated from sputum of an elderly man.

PMID: 35604945 (view PubMed database entry)
DOI: 10.1099/ijsem.0.005378 (read at publisher's website )

Yuko Abe, Kiyoharu Fukushima, Yuki Matsumoto, Takayuki Niitsu, Hiroshi Nabeshima, Yasuharu Nagahama, Eri Akiba, Kako Haduki, Haruko Saito, Tadayoshi Nitta, Akira Kawano, Michio Tanaka, Takanori Matsuki, Daisuke Motooka, Kazuyuki Tsujino, Keisuke Miki, Shota Nakamura, Tetsuya Iida, Hiroshi Kida,

A slowly growing mycobacteria, identified as strain TY59<sup>T</sup>, was isolated from sputum of an elderly man with pneumonia. Sequencing of the 16S rRNA gene indicated that this strain was similar to members of the <i>Mycobacterium avium</i> complex and closely related species. Strain TY59<sup>T</sup> has highest 16S rRNA gene sequence similarities to the type strains of <i>Mycobacterium colombiense</i> (99.80 % sequence similarity), <i>Mycobacterium vulneris</i> (99.74 %), <i>Mycobacterium timonense</i> (99.54 %), <i>Mycobacterium avium</i> subsp. <i>avium</i> (99.54 %) and <i>Mycobacterium avium</i> subsp. <i>silvaticum</i> (99.54 %). Analysis of the internal transcribed spacer (ITS) and DNA-directed RNA polymerase subunit beta (<i>rpoB</i>) sequences gave similar results to the 16S rRNA gene analysis. The closest species to strain TY59<sup>T</sup> were <i>M. colombiense</i> and <i>M. vulneris</i> with 97.90-98.25 % identity in ITS and 96.4-96.6 % in <i>rpoB</i>. The strain's 65 kDa heat shock protein (<i>hsp65</i>) gene was different from those of <i>M. vulneris</i>, <i>M. colombiense</i> and <i>M. avium</i> subsp. <i>silvaticum</i> with 72.4-74.2 % identity. Average nucleotide identity results showed a 93.4 % match to <i>M. vulneris</i> as the maximum value. Phenotypically, the non-chromogenicity, rough colonies, growth at 42 °C, negative results for nitrate reduction, β-glucosidase and Tween 80 hydrolysis, and positive results for catalase activity set this strain apart from closely related species. We propose that <i>Mycobacterium senriense</i> sp. nov. is a novel species of slowly growing mycobacteria. The type strain is TY59<sup>T</sup> (RIMD 1371001<sup>T</sup>=CIP 111917<sup>T</sup>).

Int J Syst Evol Microbiol (International journal of systematic and evolutionary microbiology)
[2022, 72(5):]

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