Full Text Journal Articles from
Abstract 32053291

Advertisement

Find full text journal articles








Establishment of recombineering genome editing system in Paraburkholderia megapolitana empowers activation of silent biosynthetic gene clusters.

PMID: 32053291 (view PubMed database entry)
DOI: 10.1111/1751-7915.13535 (read at publisher's website )
PMCID: PMC7017819 (free full text version available)

Download PDF Download PDF

Wentao Zheng, Xue Wang, Haibo Zhou, Youming Zhang, Aiying Li, Xiaoying Bian,

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, 266237, China.

The Burkholderiales are an emerging source of bioactive natural products. Their genomes contain a large number of cryptic biosynthetic gene clusters (BGCs), indicating great potential for novel structures. However, the lack of genetic tools for the most of Burkholderiales strains restricts the mining of these cryptic BGCs. We previously discovered novel phage recombinases Redαβ7029 from Burkholderiales strain DSM 7029 that could help in efficiently editing several Burkholderiales genomes and established the recombineering genome editing system in Burkholderialse species. Herein, we report the application of this phage recombinase system in another species Paraburkholderia megapolitana DSM 23488, resulting in activation of two silent non-ribosomal peptide synthetase/polyketide synthase BGCs. A novel class of lipopeptide, haereomegapolitanin, was identified through spectroscopic characterization. Haereomegapolitanin A represents an unusual threonine-tagged lipopeptide which is longer than the predicted NRPS assembly line. This recombineering-mediated genome editing system shows great potential for genetic manipulation of more Burkholderiales species to activate silent BGCs for bioactive metabolites discovery.

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):397-405]

Cited: 0 times

AltMetric Statistics
Authors' Affiliation
Map for Author Affiliation





Advertisement

Disclaimer
0.692 s