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Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to Salmonella enterica.

PMID: 22998607 (view PubMed database entry)
DOI: 10.1111/1574-6968.12010 (read at publisher's website )
PMCID: PMC3558799 (free full text version available)

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Jean Guard, Roxana Sanchez-Ingunza, Cesar Morales, Tod Stewart, Karen Liljebjelke, Joann Van Kessel, Kim Ingram, Deana Jones, Charlene Jackson, Paula Fedorka-Cray, Jonathan Frye, Richard Gast, Arthur Hinton,

Two DNA-based methods were compared for the ability to assign serotype to 139 isolates of Salmonella enterica ssp. I. Intergenic sequence ribotyping (ISR) evaluated single nucleotide polymorphisms occurring in a 5S ribosomal gene region and flanking sequences bordering the gene dkgB. A DNA microarray hybridization method that assessed the presence and the absence of sets of genes was the second method. Serotype was assigned for 128 (92.1%) of submissions by the two DNA methods. ISR detected mixtures of serotypes within single colonies and it cost substantially less than Kauffmann-White serotyping and DNA microarray hybridization. Decreasing the cost of serotyping S. enterica while maintaining reliability may encourage routine testing and research.

FEMS Microbiol. Lett. (FEMS microbiology letters)
[2012, 337(1):61-72]

Cited: 16 times

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