<i>BRR6</i> and <i>BRL1</i> are two paralogs that encode transmembrane proteins of the nuclear envelope (NE) involved in membrane fluidity and nuclear pore complex biogenesis in organisms that undergo a closed mitosis. We show that mutation of a conserved cysteine in the intralumenal domain of <i>Saccharomyces cerevisiae</i> Brr6p results in a novel temperature sensitive allele, <i>brr6-Y100H</i>, that arrests growth due to defects in spindle formation. Analysis of <i>brr6-Y100H</i> cells by electron tomography and Brr6p localization by super-resolution imaging supports the idea that Brr6p is involved in insertion of the newly duplicated spindle pole body into the NE.
MicroPubl Biol (microPublication biology)
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