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Canavanine resistance mutation can1-1 in Schizosaccharomyces pombe is a missense mutation in the ubiquitin ligase adaptor gene any1.

PMID: 35300005 (view PubMed database entry)
DOI: 10.17912/micropub.biology.000538 (read at publisher's website )
PMCID: PMC8922049 (free full text version available)

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Yu-Sheng Yang, Shao-Kai Ning, Xiao-Hui Lyu, Fang Suo, Guo-Song Jia, Wen Li, Li-Lin Du,

In Schizosaccharomyces pombe, the can1-1 mutation confers resistance to the toxic arginine analog canavanine. This mutation has been assumed to disrupt a gene encoding an arginine transporter. In PomBase, the gene SPBC18H10.16 is currently designated can1. Here, we sequenced the genomes of three can1-1 strains. No mutations were found in SPBC18H10.16. Instead, these strains harbor an R175C mutation in the gene any1 (SPBC18H10.20c). any1 encodes an α-arrestin that acts as a ubiquitin ligase adaptor to downregulate plasma membrane amino acid transporters. Our findings indicate that can1-1 is not a loss-of-function mutation in an amino acid transporter gene, but a possible gain-of-function mutation in a gene encoding a negative regulator of amino acid transporters.

MicroPubl Biol (microPublication biology)
[2022, 2022:]

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