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Gene bashing of ceh-6 locus identifies genomic regions important for ceh-6 rectal cell expression and rescue of its mutant lethality.

PMID: 33364555 (view PubMed database entry)
DOI: 10.17912/micropub.biology.000339 (read at publisher's website )
PMCID: PMC7753896 (free full text version available)

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Arnaud Ahier, Shashi Kumar Suman, Sophie Jarriault,

Strong loss-of-function or null mutants can sometimes lead to a penetrant early lethality, impairing the study of these genes' function. This is the case for the <i>ceh-6</i> null mutant, which exhibits 100% penetrant lethality. Here, we describe how we used gene bashing to identify distinct regulatory regions in the <i>ceh-6</i> locus. This allowed us to generate a <i>ceh-6</i> null strain that is viable and still displays <i>ceh-6</i> mutant Y-to-PDA transdifferentiation phenotype. Such strategy can be applied to many other mutants impacting viability.

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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