Strong loss-of-function or null mutants can sometimes lead to a penetrant early lethality, impairing the study of these genes' function. This is the case for the <i>ceh-6</i> null mutant, which exhibits 100% penetrant lethality. Here, we describe how we used gene bashing to identify distinct regulatory regions in the <i>ceh-6</i> locus. This allowed us to generate a <i>ceh-6</i> null strain that is viable and still displays <i>ceh-6</i> mutant Y-to-PDA transdifferentiation phenotype. Such strategy can be applied to many other mutants impacting viability.
MicroPubl Biol (microPublication biology)
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