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Ubiquitous Knockdown of Mettl3 using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis.

PMID: 35071998 (view PubMed database entry)
DOI: 10.17912/micropub.biology.000511 (read at publisher's website )
PMCID: PMC8767421 (free full text version available)

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Lauren Rowe, Antonio L Rockwell,

METTL3<i>,</i> the enzyme that catalyzes the m<sup>6</sup>A RNA modification in Drosophila is highly conserved and essential in various eukaryotic organisms. <i>Mettl3</i> and its homologs have been linked to biological processes such as gametogenesis. We focused on characterizing the role of METTL3 in Drosophila spermatogenesis. We used the Gal4-UAS system to ubiquitously knockdown <i>Mettl3</i> in both somatic cyst cells and germline cells. Using immunostaining and confocal microscopy, we found spermatid bundles mislocalize in testes that contain the morphologically abnormal swollen apical tip. Our result suggests <i>Mettl3</i> knockdown using TRiP.GL01126 results in spermatogenesis aberrations.

MicroPubl Biol (microPublication biology)
[2022, 2022:]

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