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Promising methods for detection of novel coronavirus SARS‐CoV‐2

Ran Liu, Aisi Fu, Zixin Deng, Yan Li, Tiangang Liu,

Abstract A very recent outbreak of the novel coronavirus, COVID‐19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections. Presently, nucleic acid amplification test is essential for the confirmation of COVID infection. In this report, ... Read more >>

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[2020, 1(1):]

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Estimation of the potential spread risk of COVID-19: Occurrence assessment along the Yangtze, Han, and Fu River basins in Hubei, China.

Bo Yang, Wei Li, Jingquan Wang, Zixin Tian, Xin Cheng, Yongli Zhang, Rui Qiu, Shuhua Hou, Hongguang Guo,

Given that the novel coronavirus was detected in stool and urine from diagnosed patients, the potential risk of its transmission through the water environment might not be ignored. In the current study, to investigate the spread possibility of COVID-19 via the environmental media, three typical rivers (Yangtze, Han, and Fu ... Read more >>

Sci Total Environ (The Science of the total environment)
[2020, 746:141353]

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Era of molecular diagnosis for pathogen identification of unexplained pneumonia, lessons to be learned.

Jing-Wen Ai, Yi Zhang, Hao-Cheng Zhang, Teng Xu, Wen-Hong Zhang,

Unexplained pneumonia (UP) caused by a novel coronavirus SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) emerged in China in late December 2019 and has infected more than 9000 cases by 31 January 2020. Shanghai reported the first imported case of COVID-19 (Coronavirus Disease 2019) in 20 January 2020. A combinative ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2020, 9(1):597-600]

Cited: 15 times

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Is There an Association Between COVID-19 Mortality and the Renin-Angiotensin System? A Call for Epidemiologic Investigations.

Thomas C Hanff, Michael O Harhay, Tyler S Brown, Jordana B Cohen, Amir M Mohareb,

Mortality from coronavirus disease 2019 (COVID-19) is strongly associated with cardiovascular disease, diabetes, and hypertension. These disorders share underlying pathophysiology related to the renin-angiotensin system (RAS) that may be clinically insightful. In particular, activity of the angiotensin-converting enzyme 2 (ACE2) is dysregulated in cardiovascular disease, and this enzyme is used ... Read more >>

Clin Infect Dis (Clinical infectious diseases : an official publication of the Infectious Diseases Society of America)
[2020, 71(15):870-874]

Cited: 31 times

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Differentiating Between 2019 Novel Coronavirus Pneumonia and Influenza Using a Nonspecific Laboratory Marker-Based Dynamic Nomogram.

Linghang Wang, Yao Liu, Ting Zhang, Yuyong Jiang, Siyuan Yang, Yanli Xu, Rui Song, Meihua Song, Lin Wang, Wei Zhang, Bing Han, Li Yang, Ying Fan, Cheng Cheng, Jingjing Wang, Pan Xiang, Lin Pu, Haofeng Xiong, Chuansheng Li, Ming Zhang, Jianbo Tan, Zhihai Chen, Jingyuan Liu, Xianbo Wang,

Background:There is currently a lack of nonspecific laboratory indicators as a quantitative standard to distinguish between the 2019 coronavirus disease (COVID-19) and an influenza A or B virus infection. Thus, the aim of this study was to establish a nomogram to detect COVID-19. Methods:A nomogram was established using data collected ... Read more >>

Open Forum Infect Dis (Open forum infectious diseases)
[2020, 7(5):ofaa169]

Cited: 1 time

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The role of a chest computed tomography severity score in coronavirus disease 2019 pneumonia.

Fausto Salaffi, Marina Carotti, Marika Tardella, Alessandra Borgheresi, Andrea Agostini, Davide Minorati, Daniela Marotto, Marco Di Carlo, Marco Di Carlo, Massimo Galli, Andrea Giovagnoni, Piercarlo Sarzi-Puttini,

The chest computed tomography (CT) characteristics of coronavirus disease 2019 (COVID-19) are important for diagnostic and prognostic purposes. The aim of this study was to investigate chest CT findings in COVID-19 patients in order to determine the optimal cut-off value of a CT severity score that can be considered a ... Read more >>

Medicine (Baltimore) (Medicine)
[2020, 99(42):e22433]

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Clinical presentations, laboratory and radiological findings, and treatments for 11,028 COVID-19 patients: a systematic review and meta-analysis.

Carlos K H Wong, Janet Y H Wong, Eric H M Tang, C H Au, Abraham K C Wai,

This systematic review and meta-analysis investigated the comorbidities, symptoms, clinical characteristics and treatment of COVID-19 patients. Epidemiological studies published in 2020 (from January-March) on the clinical presentation, laboratory findings and treatments of COVID-19 patients were identified from PubMed/MEDLINE and Embase databases. Studies published in English by 27th March, 2020 with original ... Read more >>

Sci Rep (Scientific reports)
[2020, 10(1):19765]

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Supplementation with vitamin D in the COVID-19 pandemic?

Fatemeh Hadizadeh,

The coronavirus disease 2019 (COVID-19) pandemic was declared a public health emergency of international concern by the World Health Organization. COVID-19 has high transmissibility and could result in acute lung injury in a fraction of patients. By counterbalancing the activity of the renin-angiotensin system, angiotensin-converting enzyme 2, which is the ... Read more >>

Nutr Rev (Nutrition reviews)
[2021, 79(2):200-208]

Cited: 1 time

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Sensitivity in Detection of Antibodies to Nucleocapsid and Spike Proteins of Severe Acute Respiratory Syndrome Coronavirus 2 in Patients With Coronavirus Disease 2019.

Peter D Burbelo, Francis X Riedo, Chihiro Morishima, Stephen Rawlings, Davey Smith, Sanchita Das, Jeffrey R Strich, Daniel S Chertow, Richard T Davey, Jeffrey I Cohen,

<h4>Background</h4>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of coronavirus disease 2019 (COVID-19), is associated with respiratory-related disease and death. Assays to detect virus-specific antibodies are important to understand the prevalence of infection and the course of the immune response.<h4>Methods</h4>Quantitative measurements of plasma or serum antibodies to the nucleocapsid ... Read more >>

J Infect Dis (The Journal of infectious diseases)
[2020, 222(2):206-213]

Cited: 6 times

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Traditional Usage of Wild Fauna among the Local Inhabitants of Ladakh, Trans-Himalayan Region.

Shiekh Marifatul Haq, Eduardo Soares Calixto, Umer Yaqoob, Riyaz Ahmed, Ahmed Hossam Mahmoud, Rainer W Bussmann, Osama B Mohammed, Khalid Ahmad, Arshad Mehmood Abbasi,

Zootherapy is accepted all around the globe not only in ancient cultures but different animal derived medicines are also part of the practice in the modern health care systems. The present study assessed the traditional ethnozoological usage of wild animals by local inhabitants in Ladakh region, India, and the reference ... Read more >>

Animals (Basel) (Animals : an open access journal from MDPI)
[2020, 10(12):]

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Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients.

Yong Xiong, Yuan Liu, Liu Cao, Dehe Wang, Ming Guo, Ao Jiang, Dong Guo, Wenjia Hu, Jiayi Yang, Zhidong Tang, Honglong Wu, Yongquan Lin, Meiyuan Zhang, Qi Zhang, Mang Shi, Yingle Liu, Yu Zhou, Ke Lan, Yu Chen,

Circulating in China and 158 other countries and areas, the ongoing COVID-19 outbreak has caused devastating mortality and posed a great threat to public health. However, efforts to identify effectively supportive therapeutic drugs and treatments has been hampered by our limited understanding of host immune response for this fatal disease. ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2020, 9(1):761-770]

Cited: 115 times

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Metagenomic Next-Generation Sequencing of Nasopharyngeal Specimens Collected from Confirmed and Suspect COVID-19 Patients.

Heba H Mostafa, John A Fissel, Brian Fanelli, Yehudit Bergman, Victoria Gniazdowski, Manoj Dadlani, Karen C Carroll, Rita R Colwell, Patricia J Simner,

Metagenomic next-generation sequencing (mNGS) offers an agnostic approach for emerging pathogen detection directly from clinical specimens. In contrast to targeted methods, mNGS also provides valuable information on the composition of the microbiome and might uncover coinfections that may associate with disease progression and impact prognosis. To evaluate the use of ... Read more >>

mBio (mBio)
[2020, 11(6):]

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RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak.

Liangjun Chen, Weiyong Liu, Qi Zhang, Ke Xu, Guangming Ye, Weichen Wu, Ziyong Sun, Fang Liu, Kailang Wu, Bo Zhong, Yi Mei, Wenxia Zhang, Yu Chen, Yirong Li, Mang Shi, Ke Lan, Yingle Liu,

From December 2019, an outbreak of unusual pneumonia was reported in Wuhan with many cases linked to Huanan Seafood Market that sells seafood as well as live exotic animals. We investigated two patients who developed acute respiratory syndromes after independent contact history with this market. The two patients shared common ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2020, 9(1):313-319]

Cited: 76 times

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Identification of the RNase-binding site of SARS-CoV-2 RNA for anchor primer-PCR detection of viral loading in 306 COVID-19 patients.

Tao Xu, Jingu Wang, Bingjie Hu, Guosi Zhang, Wu Zhou, Meiqin Zheng, Bo Shen, Baochang Sun, Yanjun Zhang, Yin Chen, Jian Yu, Min Liang, Jingye Pan, Chengshui Chen, Haixiao Chen, Minghua Jiang, Liangde Xu, Jia Qu, Jiang-Fan Chen,

The pandemic of coronavirus disease 2019 (COVID-19) urgently calls for more sensitive molecular diagnosis to improve sensitivity of current viral nuclear acid detection. We have developed an anchor primer (AP)-based assay to improve viral RNA stability by bioinformatics identification of RNase-binding site of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ... Read more >>

Brief Bioinform (Briefings in bioinformatics)
[2020, :]

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Laboratory Diagnosis of COVID-19: Current Issues and Challenges.

Yi-Wei Tang, Jonathan E Schmitz, David H Persing, Charles W Stratton,

The COVID-19 outbreak has had a major impact on clinical microbiology laboratories in the past several months. This commentary covers current issues and challenges for the laboratory diagnosis of infections caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In the preanalytical stage, collecting the proper respiratory tract specimen at ... Read more >>

J Clin Microbiol (Journal of clinical microbiology)
[2020, 58(6):]

Cited: 108 times

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Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated In Vitro and with Clinical Specimens.

Jasper Fuk-Woo Chan, Cyril Chik-Yan Yip, Kelvin Kai-Wang To, Tommy Hing-Cheung Tang, Sally Cheuk-Ying Wong, Kit-Hang Leung, Agnes Yim-Fong Fung, Anthony Chin-Ki Ng, Zijiao Zou, Hoi-Wah Tsoi, Garnet Kwan-Yue Choi, Anthony Raymond Tam, Vincent Chi-Chung Cheng, Kwok-Hung Chan, Owen Tak-Yin Tsang, Kwok-Yung Yuen,

On 31 December 2019, the World Health Organization was informed of a cluster of cases of pneumonia of unknown etiology in Wuhan, China. Subsequent investigations identified a novel coronavirus, now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), from the affected patients. Highly sensitive and specific laboratory diagnostics are important ... Read more >>

J Clin Microbiol (Journal of clinical microbiology)
[2020, 58(5):]

Cited: 119 times

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COVID-19 diagnostic testing: Technology perspective.

Meng Xu, Dan Wang, Hongye Wang, Xiaomei Zhang, Te Liang, Jiayu Dai, Meng Li, Jiahui Zhang, Kai Zhang, Danke Xu, Xiaobo Yu,

The corona virus disease 2019 (COVID-19) is a highly contagious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). More than 18 million people were infected with a total of 0.7 million deaths in ∼188 countries. Controlling the spread of SARS-CoV-2 is therefore inherently dependent on identifying and ... Read more >>

Clin Transl Med (Clinical and translational medicine)
[2020, 10(4):e158]

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Unique epidemiological and clinical features of the emerging 2019 novel coronavirus pneumonia (COVID-19) implicate special control measures.

Yixuan Wang, Yuyi Wang, Yan Chen, Qingsong Qin,

By 27 February 2020, the outbreak of coronavirus disease 2019 (COVID-19) caused 82 623 confirmed cases and 2858 deaths globally, more than severe acute respiratory syndrome (SARS) (8273 cases, 775 deaths) and Middle East respiratory syndrome (MERS) (1139 cases, 431 deaths) caused in 2003 and 2013, respectively. COVID-19 has spread to ... Read more >>

J Med Virol (Journal of medical virology)
[2020, 92(6):568-576]

Cited: 189 times

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Coronavirus Disease 2019 (COVID-19) pandemic, lessons to be learned!

Md Saiful Islam, Md Abdus Sobur, Mily Akter, K H M Nazmul Hussain Nazir, Antonio Toniolo, Md Tanvir Rahman,

Coronavirus Disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been reported as a worldwide emergency. Due to the extensiveness of spread and death, it has been declared as a pandemic. This review focused on the current pandemic situation and understanding the prevention and control strategies ... Read more >>

J Adv Vet Anim Res (Journal of advanced veterinary and animal research)
[2020, 7(2):260-280]

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