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Two plasmid modules for introducing the auxin-inducible degron into the fission yeast Schizosaccharomyces pombe by PCR-based gene targeting.

Xiuyi Song, Ruoming Xu, Tomoyasu Sugiyama,

Targeted protein degradation is a powerful approach to study and inhibit protein function <i>in vivo</i>. Introduction of the auxin-inducible degron (AID) system to the fission yeast <i>Schizosaccharomyces pombe</i> was previously reported, but, to the best of our knowledge, no plasmid for constructing AID-tagged fission yeast strains has been described so ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Live-cell imaging reveals square shape spindles and long mitosis duration in the silkworm holocentric cells.

Lucien Vanpoperinghe, Frederique Carlier-Grynkorn, Gaetan Cornilleau, Takahiro Kusakabe, Ines A Drinnenberg, Phong T Tran,

Proper chromosome segregation during mitosis requires both the assembly of a microtubule (MT)-based spindle and the assembly of DNA-centromere-based kinetochore structure. Kinetochore-to-MT attachment enables chromosome separation. Monocentric cells, such as found in human, have one unique kinetochore per chromosome. Holocentric cells, such as found in the silkworm, in contrast, have ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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SIMR foci are found in the progenitor germ cells of C. elegans embryos.

Celja J Uebel, Kevin I Manage, Carolyn M Phillips,

RNA interference is a widely conserved mechanism of gene regulation and silencing across eukaryotes. In <i>C. elegans</i>, RNA silencing is coordinated through perinuclear nuage containing at least four granules: P granules, Z granules, <i>Mutator</i> foci, and SIMR foci. Embryonic localization of these granules is known for all except SIMR foci. ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane.

Jun Takayama, Tatsuya Tajima, Shuichi Onami, Hitoshi Nishimura,

<i>C. elegans spe-9</i> class genes encode sperm proteins with indispensable roles during fertilization. We have previously reported that <i>spe-45</i> belongs to the <i>spe-9</i> class, based on the finding that self-sperm of <i>spe-45(tm3715)</i> hermaphrodites were not consumed by fertilization. In this study, we directly observed live fertilization in the spermatheca of ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Depletion of Foxk transcription factors causes genome-wide transcriptional misregulation and developmental arrest in zebrafish embryos.

Fan-Suo Geng, Elisa de la Calle-Mustienes, José Luis Gómez-Skarmeta, Ryan Lister, Ozren Bogdanovic,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Expression of a FRET-based ATP Biosensor in the C. elegans Intestine.

Jose Soto, Madeline Rivera, Gina Broitman-Maduro, Morris F Maduro,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Loss of fuss in Drosophila melanogaster results in decreased locomotor activity due to an increased number of pauses.

Mathias Rass, Svenja Oestreich, Ardi Manaj, Stephan Schneuwly,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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The root hair defective phenotype of Arabidopsis thaliana Pol IV subunit mutant nrpd1a-3 is associated with a deletion in RHD6.

Rakesh David, R Kortschak, Iain Searle,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

Cited: 1 time

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Protease cleavage at an engineered tetra-basic motif in Drosophila PTTH accelerates developmental timing.

MaryJane Shimell, Michael O'Connor,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

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Microglia regulation of synaptic plasticity and learning and memory.

Jessica Cornell, Shelbi Salinas, Hou-Yuan Huang, Miou Zhou,

Microglia are the resident macrophages of the central nervous system. Microglia possess varied morphologies and functions. Under normal physiological conditions, microglia mainly exist in a resting state and constantly monitor their microenvironment and survey neuronal and synaptic activity. Through the C1q, C3 and CR3 "Eat Me" and CD47 and SIRPα ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(4):705-716]

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Underlying reasons why some people haven't tested for HIV - a discourse analysis of qualitative data from Cape Town, South Africa.

Kyla Meyerson, Graeme Hoddinott, Tamryn Nicholson, Sue-Ann Meehan,

Reported barriers to HIV testing over the last 15 years have remained consistent, despite improved service offerings. We aimed to probe deeper by exploring how people who have never tested construct HIV testing in their talk. We used this to suggest underlying psychosocial barriers to testing even when there is ... Read more >>

SAHARA J (SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance)
[2021, 18(1):105-112]

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Incidence of socially significant infectious diseases (HIV, TB and HIV/TB coinfection) in the Arctic regions of Russia).

Z Zagdyn, Y Zhao, V Tsvetkov, S Sleptsova, M Vinokurova, E Sokolovich, P Yablonskiy,

The study is aimed to evaluate the HIV, TB, and HIV/TB coinfection incidence per 100,000 population/year in Russian Arctic, based on official statistical data. The epidemics' incidence in Russian Arctic is uneven. The highest HIV incidence in 2019 was registered in Krasnoyarsk region (94,6), and the highest TB incidence in ... Read more >>

Int J Circumpolar Health (International journal of circumpolar health)
[2021, 80(1):1966924]

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Traditional healers as client advocates in the HIV-endemic region of Maputo, Mozambique: results from a qualitative study.

Radhika Sundararajan, Patricio V Langa, Trisha Morshed, Sandra Manuel,

Traditional healers are commonly utilised throughout sub-Saharan Africa instead of - and in concert with - biomedical facilities. Traditional healers are trusted providers and prominent community members and could be important partners in improving engagement with HIV services in endemic contexts. Our study sought to understand the roles of healers ... Read more >>

SAHARA J (SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance)
[2021, 18(1):77-85]

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Making sense of fidelity: young Africans' cross-national and longitudinal representations of fidelity and infidelity in their HIV-related creative narratives, 1997-2014.

Robyn Singleton, Manon Billaud, Haley McLeod, Georges Tiendrebeogo, Fatim Dia, Chris Obong'o, Siphiwe Nkambule-Vilakati, Benjamin Mbakwem, Gaelle Sabben, Kate Winskell,

Mutual fidelity and partner reduction have been identified as key behavioural strategies to prevent HIV transmission in sub-Saharan Africa, particularly following recognition of the role that multiple concurrent sexual partnerships play in driving generalised HIV epidemics. We analysed social representations of fidelity and infidelity in a sample of 1,343 narratives ... Read more >>

SAHARA J (SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance)
[2021, 18(1):86-97]

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HIV/AIDS treatment funding system to support the people affected by HIV/AIDS in Surakarta, Indonesia.

Argyo Demartoto, Bhisma Murti, Siti Zunariyah,

People Living with HIV/AIDS (PLWHA's) quality of life (QoL) is determined by the lifetime treatment sustainability. Republic of Indonesia Minister of Health's Decree Number 328 of 2003 stated that government subsidies the PLWHA's medication and treatment, despite not covering entire medication and treatment cost. The objective of research was to ... Read more >>

SAHARA J (SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance)
[2021, 18(1):1-16]

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Knowledge, attitudes and prevention practices regarding HIV/AIDS among barbers in Ho municipality, Ghana.

Mercy Demaris Quarm, Jacqueline Mthembu, Khangelani Zuma, Elvis Enowbeyang Tarkang,

The barbing industry poses particular public health risks if it is not conducted in a safe and hygienic manner. These risks can lead to the transmission of infectious diseases like the Human Immunodeficiency Virus (HIV) to the barbers or their clients. This study investigated the knowledge, attitudes and prevention practices ... Read more >>

SAHARA J (SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance)
[2021, 18(1):42-51]

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Discussing reproductive goals with healthcare providers among women living with HIV in Canada: the role of provider gender and patient comfort.

Lashanda Skerritt, Alexandra de Pokomandy, Nadia O'Brien, Nadia Sourial, Ann N Burchell, Gillian Bartlett, Tibor Schuster, Danielle Rouleau, Karène Proulx-Boucher, Neora Pick, Deborah Money, Rebecca Gormley, Allison Carter, Mark H Yudin, Mona Loutfy, Angela Kaida, ,

Antiretroviral therapy effectively prevents sexual and vertical transmission of HIV. Yet, some women living with HIV report having unmet needs for reproductive health care. This study measured the prevalence of women discussing reproductive goals with any current healthcare provider and assessed the effect of the current HIV care provider's gender ... Read more >>

Sex Reprod Health Matters (Sexual and reproductive health matters)
[2021, 29(1):1932702]

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'If you are found taking medicine, you will be called names and considered less of a man': young men's engagement with HIV treatment and care during ulwaluko (traditional initiation and circumcision) in the Eastern Cape Province of South Africa.

L Gittings, R Hodes, C Colvin, S Mbula, P Kom,

This paper explores how HIV-positive <i>abakhwetha</i> (young male initiates) undergoing <i>ulwaluko</i> (traditional Xhosa initiation and circumcision) engage with HIV-related biomedical care and treatment. Health-focused life history narratives (<i>n</i> = 36), semi-structured interviews (<i>n</i> = 32) and analysis of health facility files (<i>n</i> = 41) with adolescent boys and young men (ages 13-24) living with HIV, and ... Read more >>

SAHARA J (SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance)
[2021, 18(1):64-76]

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Maternal and infant antiretroviral therapy adherence among women living with HIV in rural South Africa: a cluster randomised trial of the role of male partner participation on adherence and PMTCT uptake.

Deborah L Jones, Violeta J Rodriguez, Manasi Soni Parrish, Tae Kyoung Lee, Stephen M Weiss, Shandir Ramlagan, Karl Peltzer,

'Mother-to-child transmission of HIV' can occur during the period of pregnancy, childbirth, or breastfeeding. 'Prevention of mother-to-child transmission of HIV' (PMTCT) in Mpumalanga Province, South Africa, is especially vital as the prevalence of HIV is 28.2% in women aged 15-49. PMTCT interventions resulted in a drop of MTCT rates in ... Read more >>

SAHARA J (SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance)
[2021, 18(1):17-25]

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The inference of HIV-1 transmission direction between a man who has sex with men and his heterosexual wife based on the sequences of HIV-1 quasi-species.

Zehua Zhou, Ping Ma, Yi Feng, Weidong Ou, Min Wei, Yiming Shao,

Currently, homosexual transmission has become one of the main routes of HIV-1 spread in China. Furthermore, about 80% Chinese men, who have sex with men (MSM), feel forced to enter eventually into heterosexual marriages due to the Chinese traditional marriage culture, which may cause HIV-1 infection in families. In this ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):1209-1216]

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A Matter of Fat: Body Fat Distribution and Cardiometabolic Disease in Africa.

Nasrin Goolam Mahyoodeen, Nigel J Crowther,

Africa is a complex and diverse continent that faces numerous challenges. It is a region in epidemiological transition which is currently experiencing a dual burden of communicable and non-communicable diseases. The high prevalence of cardiometabolic disease (CMD) on the continent is driven largely by the increasing prevalence of obesity in ... Read more >>

Methods Mol Biol (Methods in molecular biology (Clifton, N.J.))
[2022, 2343:37-56]

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Epidemiological profile and molecular genetic characterization of HIV-1 among female sex workers and elderly male clients in Guangxi, China.

Xiu Chen, Chunwei Qin, Rongfeng Chen, Yunxuan Huang, Yuexiang Xu, Qiao Tang, Xianjun Liang, Bing Peng, Yi Shao, Yao Yang, Jie Chen, Xinwei Wang, Liufang Wen, Bingyu Liang, Li Ye, Xionglin Qin, Junjun Jiang, Hao Liang,

The HIV/AIDS prevalence in female sex workers (FSWs) and elderly male clients is increasing in Guangxi, China, but the transmission relationship between them remains unclear. This study aims to illuminate the transmission network between FSWs and elderly male clients using molecular epidemiological analyses. Phylogenetic analysis indicated that CRF01_AE was the ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):384-395]

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Pan-coronavirus fusion inhibitors possess potent inhibitory activity against HIV-1, HIV-2, and simian immunodeficiency virus.

Danwei Yu, Yuanmei Zhu, Hongxia Yan, Tong Wu, Huihui Chong, Yuxian He,

EK1 peptide is a membrane fusion inhibitor with broad-spectrum activity against human coronaviruses (CoVs). In the outbreak of COVID-19, we generated a lipopeptide EK1V1 by modifying EK1 with cholesterol, which exhibited significantly improved antiviral activity. In this study, we surprisingly found that EK1V1 also displayed potent cross-inhibitory activities against divergent ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):810-821]

Cited: 2 times

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Project Last Mile and the development of the Girl Champ brand in eSwatini: engaging the private sector to promote uptake of health services among adolescent girls and young women.

Marie A Brault, Sarah Christie, Sasha Aquino, Abigail Rendin, Amanda Manchia, Leslie A Curry, Erika L Linnander,

In eSwatini and across sub-Saharan Africa, adolescent girls and young women (AGYW) are at significantly higher risk of HIV infection and poorer sexual and reproductive health (SRH) than their male counterparts. AGYW demonstrate low demand for SRH services, further contributing to poor outcomes. Strategic marketing approaches, including those used by ... Read more >>

SAHARA J (SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance)
[2021, 18(1):52-63]

Cited: 1 time

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Using molecular transmission networks to understand the epidemic characteristics of HIV-1 CRF08_BC across China.

Kang Li, Meiliang Liu, Huanhuan Chen, Jianjun Li, Yanling Liang, Yi Feng, Hui Xing, Yiming Shao,

HIV-1 CRF08_BC has become a major epidemic in heterosexuals and intravenous drug users (IDUs) in southern China. In order to evaluate the trends of its epidemic and facilitate targeted HIV prevention, we constructed the genetic transmission networks based on its <i>pol</i> sequences, derived from the National HIV Molecular Epidemiology Survey. ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):497-506]

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